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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
09/05/2022 |
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Data da última atualização: |
09/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AMARAL, T. F.; GRAZIA, J. G. V. de; MARTINHAO, L A. G.; DE COL, F.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; HANSEN, P. J. |
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Afiliação: |
THIAGO F. AMARAL, University of Florida; JOAO GABRIEL VIANA DE GRAZIA, FIVX Apoyar Biotech Ltda; LUANY ALVES GALVAO MARTINHAO, FIVX Apoyar Biotech Ltda; FELIPE DE COL, FIVX Apoyar Biotech Ltda; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen; PETER J. HANSEN, University of Florida. |
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Título: |
Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Scientific Reports, v. 12, 7503, 2022. |
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DOI: |
https://doi.org/10.1038/s41598-022-11447-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Procedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro while also compromising competence of embryos to survive after transfer, (2) actions of CSF2 and DKK1 depend upon other characteristics of the embryo production system, and (3) sire can have a large efect on embryonic development before and after transfer. MenosProcedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro w... Mostrar Tudo |
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Palavras-Chave: |
Fertilização; Produção in vitro. |
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Thesagro: |
Bovino; Embrião; Reprodução Animal. |
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Thesaurus Nal: |
Animal reproduction; Embryo (animal); In vitro fertilization. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142749/1/Actions-of-CSF2.pdf
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Marc: |
LEADER 02687naa a2200301 a 4500
001 2142749
005 2022-05-09
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-022-11447-7$2DOI
100 1 $aAMARAL, T. F.
245 $aActions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium.$h[electronic resource]
260 $c2022
520 $aProcedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro while also compromising competence of embryos to survive after transfer, (2) actions of CSF2 and DKK1 depend upon other characteristics of the embryo production system, and (3) sire can have a large efect on embryonic development before and after transfer.
650 $aAnimal reproduction
650 $aEmbryo (animal)
650 $aIn vitro fertilization
650 $aBovino
650 $aEmbrião
650 $aReprodução Animal
653 $aFertilização
653 $aProdução in vitro
700 1 $aGRAZIA, J. G. V. de
700 1 $aMARTINHAO, L A. G.
700 1 $aDE COL, F.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aVIANA, J. H. M.
700 1 $aHANSEN, P. J.
773 $tScientific Reports$gv. 12, 7503, 2022.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 9 | |
| 1. |  | RAGALZI, C. M.; VENTURA, E. F.; RESENDE, R. M. S. Otimização de protocolos de PCR em multiplex para análise de fragmentos por eletroforese capilar em Brachiaria. WORKSHOP MELHORAMENTO VEGETAL, 2., 2016, Campo Grande, MS. Contribuições, Avanços e perspectivas para o Cerrado brasileiro. Anais... Campo Grande, MS: SBMP, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 2. | | RAGALZI, C. M.; RESENDE, R. M. S.; VILELA, M. de M.; VENTURA, E. F.; JANK, L. Associação genômica ampla na seleção para apomixia em Panicum maximum jacq. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 11., 2015, Campo Grande, MS. Anais... Campo Grande: Embrapa Gado de Corte, 2015. 114 p. (Embrapa Gado de Corte. Documentos, 213).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 3. | | VENTURA, E. F.; RESENDE, R. M. S.; RAGALZI, C. M.; VILELA, M. de M. Distribuição da variação genética entre e dentro de sub-populações de Brachiaria ruziziensis tetraploide e sua aplicação na seleção direcional. WORKSHOP MELHORAMENTO VEGETAL, 2., 2016, Campo Grande, MS. Contribuições, Avanços e perspectivas para o Cerrado brasileiro. Anais... Campo Grande, MS: SBMP, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 5. | | PEREIRA, T. B. C.; FERNANDES, C. D.; VERZIGNASSI, J. R.; BATISTA, M. V.; QUETEZ, F. A.; VENTURA, E. F. Efeito de populações crescentes de Pratylenchus brachyurus no desempenho agronômico de Brachiaria brizantha cv. Piatã. Summa Phytopathologica, v. 43, Feb. 2017. Supplement. Resumos do Congresso Paulista de Fitopatologia, 40., 2017, Campinas.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 6. | | VENTURA, E. F.; FERNANDES, C. D.; JANK, L.; VERZIGNASSI, J. R.; SANTOS, M. F.; QUETEZ, F. A.; BATISTA, M. V. Reação de genótipos de Panicum maximum à mancha foliar. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 12., 2016, Campo Grande, MS. Anais... Brasília, DF: Embrapa Gado de Corte, 2016. 112 p. (Embrapa Gado de Corte. Documentos, 227). Comissão organizadora: Marta Pereira da Silva - Coordenadora; Mateus Figueiredo Santos - Vice-Coordenador; Rodrigo Carvalho Alva - Secretário Executivo e Editoração.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 7. | | PEREIRA, T. B. C.; FERNANDES, C. D.; VERZIGNASSI, J. R.; BATISTA, M. V.; QUETEZ, F. A.; VENTURA, E. F. Efeito do tratamento de sementes para controle de Bipolaris maydis em duas cultivares de Panicum maximum. Summa Phytopathologica, v. 43, Feb. 2017. Supplement. Resumos do Congresso Paulista de Fitopatologia, 40., 2017, Campinas.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 8. | | PEREIRA, T. B. C.; FERNANDES, C. D.; JANK, L.; VERZIGNASSI, J. R.; QUETEZ, F. A.; BATISTA, M. V.; VENTURA, E. F. Avaliação da resistência de genótipos de Panicum maximum à mancha foliari. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 50., 2017, Uberlândia. Do manejo à edição do genoma: resumos. Brasília, DF: SBF, 2017. Título em inglês: Evaliation of resistance of Panicum maximum genotypes to leaf spot.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 9. | | VENTURA, E. F.; RAGALZI, C. M.; RESENDE, R. M. S.; TORRES, F. Z. V.; VILELA, M. de M.; BARRIOS, S. C. L.; VALLE, C. B. do. Seleção genômica ampla para resistência a Notozulia entreriana e modo de reprodução em Brachiaria. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 11., 2015, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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