|
|
Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
11/09/2017 |
Data da última atualização: |
06/04/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CAMPOS, C. G.; VERAS, H. C. T.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; ARAÚJO, K. P.; RODRIGUES, C. M.; ALMEIDA, J. R. M. de; ABDELNUR, P. V. |
Afiliação: |
CHRISTIANE GONÇALVES CAMPOS, Universidade Federal de Goiás; HENRIQUE CÉSAR TEIXEIRA VERAS, Universidade de Brasília; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; PATRICIA PINTO KALIL G COSTA, CNPAE; KATIÚSCIA PEREIRA ARAÚJO; CLENILSON MARTINS RODRIGUES, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE. |
Título: |
New protocol based on UHPLC-MS/MS for quantitation of metabolites in xylose-fermenting yeasts. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Journal of the American Society for Mass Spectrometry, v. 28, n. 12, p. 2646-2657, 2017. |
Páginas: |
p. 2646-2657 |
DOI: |
10.1007/s13361-017-1786-9 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Xylose fermentation is a bottleneck in second-generation ethanol production. As such, a comprehensive understanding of xylose metabolism in naturally xylose-fermenting yeasts is essential for prospection and construction of recombinant yeast strains. The objective of the current study was to establish a reliable metabolomics protocol for quantification of key metabolites of xylose catabolism pathways in yeast, and to apply this protocol to Spathaspora arborariae. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites, and afterwards, sample preparation was optimized to examine yeast intracellular metabolites. S. arborariae was cultivated using xylose as a carbon source under aerobic and oxygen-limited conditions. Ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) were shown to efficiently quantify 14 and 5 metabolites, respectively, in a more rapid chromatographic protocol than previously described. Thirteen and eleven metabolites were quantified in S. arborariae under aerobic and oxygen-limited conditions, respectively. This targeted metabolomics protocol is shown here to quantify a total of 19 metabolites, including sugars, phosphates, coenzymes, monosaccharides, and alcohols, from xylose catabolism pathways (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle) in yeast. Furthermore, to our knowledge, this is the first time that intracellular metabolites have been quantified in S. arborariae after xylose consumption. The results indicated that fine control of oxygen levels during fermentation is necessary to optimize ethanol production by S. arborariae. The protocol presented here may be applied to other yeast species and could support yeast genetic engineering to improve second generation ethanol production. Graphical Abstract ?. MenosAbstract: Xylose fermentation is a bottleneck in second-generation ethanol production. As such, a comprehensive understanding of xylose metabolism in naturally xylose-fermenting yeasts is essential for prospection and construction of recombinant yeast strains. The objective of the current study was to establish a reliable metabolomics protocol for quantification of key metabolites of xylose catabolism pathways in yeast, and to apply this protocol to Spathaspora arborariae. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites, and afterwards, sample preparation was optimized to examine yeast intracellular metabolites. S. arborariae was cultivated using xylose as a carbon source under aerobic and oxygen-limited conditions. Ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) were shown to efficiently quantify 14 and 5 metabolites, respectively, in a more rapid chromatographic protocol than previously described. Thirteen and eleven metabolites were quantified in S. arborariae under aerobic and oxygen-limited conditions, respectively. This targeted metabolomics protocol is shown here to quantify a total of 19 metabolites, including sugars, phosphates, coenzymes, monosaccharides, and alcohols, from xylose catabolism pathways (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle) in yeast. Furthermore, to our knowledge, this is... Mostrar Tudo |
Palavras-Chave: |
Spathaspora arborariae; UHPLC-MS/MS; Xylose fermentation. |
Thesaurus Nal: |
Mass spectrometry; Metabolomics; Xylose. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163703/1/Abdelnur-ASMassSoectiometry-2017.pdf
|
Marc: |
LEADER 02860naa a2200301 a 4500 001 2075378 005 2021-04-06 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1007/s13361-017-1786-9$2DOI 100 1 $aCAMPOS, C. G. 245 $aNew protocol based on UHPLC-MS/MS for quantitation of metabolites in xylose-fermenting yeasts.$h[electronic resource] 260 $c2017 300 $ap. 2646-2657 520 $aAbstract: Xylose fermentation is a bottleneck in second-generation ethanol production. As such, a comprehensive understanding of xylose metabolism in naturally xylose-fermenting yeasts is essential for prospection and construction of recombinant yeast strains. The objective of the current study was to establish a reliable metabolomics protocol for quantification of key metabolites of xylose catabolism pathways in yeast, and to apply this protocol to Spathaspora arborariae. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites, and afterwards, sample preparation was optimized to examine yeast intracellular metabolites. S. arborariae was cultivated using xylose as a carbon source under aerobic and oxygen-limited conditions. Ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) were shown to efficiently quantify 14 and 5 metabolites, respectively, in a more rapid chromatographic protocol than previously described. Thirteen and eleven metabolites were quantified in S. arborariae under aerobic and oxygen-limited conditions, respectively. This targeted metabolomics protocol is shown here to quantify a total of 19 metabolites, including sugars, phosphates, coenzymes, monosaccharides, and alcohols, from xylose catabolism pathways (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle) in yeast. Furthermore, to our knowledge, this is the first time that intracellular metabolites have been quantified in S. arborariae after xylose consumption. The results indicated that fine control of oxygen levels during fermentation is necessary to optimize ethanol production by S. arborariae. The protocol presented here may be applied to other yeast species and could support yeast genetic engineering to improve second generation ethanol production. Graphical Abstract ?. 650 $aMass spectrometry 650 $aMetabolomics 650 $aXylose 653 $aSpathaspora arborariae 653 $aUHPLC-MS/MS 653 $aXylose fermentation 700 1 $aVERAS, H. C. T. 700 1 $aRIBEIRO, J. A. de A. 700 1 $aCOSTA, P. P. K. G. 700 1 $aARAÚJO, K. P. 700 1 $aRODRIGUES, C. M. 700 1 $aALMEIDA, J. R. M. de 700 1 $aABDELNUR, P. V. 773 $tJournal of the American Society for Mass Spectrometry$gv. 28, n. 12, p. 2646-2657, 2017.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agroenergia (CNPAE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
27/08/2012 |
Data da última atualização: |
27/08/2012 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MIRANDA, M. Z. de; CAETANO, V. da R.; SCHEEREN, P. L.; TIGGEMANN, L.; TATSCH, P. O.; ORO, T. |
Afiliação: |
MARTHA ZAVARIZ DE MIRANDA, CNPT; VANDERLEI DA ROSA CAETANO, CPACT; PEDRO LUIZ SCHEEREN, CNPT; Lídia Tiggemann, URI, Erechim-RS, Brazil.; PIHETRA OLIVEIRA TATSCH, CNPT; Tatiana Oro, UFSC, Florianópolis-SC, Brazil. |
Título: |
Relationship between wheat gluten color and wheat quality: preliminary results. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves, Brasil. Bento Gonçalves: International Crop Science Society, 2012. |
Idioma: |
Inglês |
Palavras-Chave: |
Relationship. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/65000/1/MARTHA-Abstract-gluten-x-color-2.pdf
|
Marc: |
LEADER 00602nam a2200169 a 4500 001 1932220 005 2012-08-27 008 2012 bl uuuu u00u1 u #d 100 1 $aMIRANDA, M. Z. de 245 $aRelationship between wheat gluten color and wheat quality$bpreliminary results.$h[electronic resource] 260 $aINTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves, Brasil. Bento Gonçalves: International Crop Science Society$c2012 653 $aRelationship 700 1 $aCAETANO, V. da R. 700 1 $aSCHEEREN, P. L. 700 1 $aTIGGEMANN, L. 700 1 $aTATSCH, P. O. 700 1 $aORO, T.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Clima Temperado (CPACT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|