Data/hora: 18/06/2024 13:16:50

Biblioteca(s): | Embrapa Pecuária Sudeste.
Data corrente: | 29/06/2021
Data da última atualização: | 06/12/2021
Autoria: | GIGLIOTI, R.; OKINO, C. H.; AZEVEDO, B. T.; WEDY, B. C. R.; GUTMANIS, G.; VERÍSSIMO, C. J.; KATIKI, L. M.; VERCESI FILHO, A. E.; OLIVEIRA, H. N. DE; OLIVEIRA, M. C. de S.
Afiliação: | RODRIGO GIGLIOTI, IZ; CINTIA HIROMI OKINO, CPPSE; BIANCA TAINA AZEVEDO, IZ; BRUNA COSTA RODRIGUES WEDY, IZ; GUNTA GUTMANIS, IZ; CECÍLIA JOSE VERÍSSIMO, IZ; LUCIANA MORITA KATIKI, IZ; ANIBAL EUGENIO VERCESI FILHO, IZ; HENRIQUE NUNES DE OLIVEIRA, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE.
Título: | Semi-quantitative evaluation of Babesia bovis and B. bigemina infection levels estimated by HRM analysis.
Ano de publicação: | 2021
Fonte/Imprenta: | Ticks and Tick-borne Diseases, v.12, n.5, 101753, sep. 2021.
Páginas: | 6 p.
DOI: | https://doi.org/10.1016/j.ttbdis.2021.101753
Idioma: | Inglês
Conteúdo: | Bovine babesiosis is economically the most important arthropod-borne disease of cattle worldwide. The most significant damage caused by bovine babesiosis is attributed to Babesia bovis due to its higher pathogenicity. This study aimed to develop a real-time PCR method followed by HRM (high-resolution melting) analysis for the simultaneous detection of B. bovis and B. bigemina, enabling a semi-quantitative analysis of Babesia levels using a single-tube reaction. The HRM was compared with real-time PCR using species-specific hydrolysis probes. The HRM analysis allowed to differentiate both Babesia species and was sensitive in the detection and differentiation of 10% for each Babesia species in the sample. Our results suggest the use of this method to estimate the prevalence of infections by B. bovis or B. bigemina as an alternative to the methods of absolute quantification by real-time PCR since it neither requires precise estimates of the number of DNA loads nor the construction of calibration curves. The simultaneous detection of the two Babesia species can be used to characterise the infection levels in cattle populations from different geographical regions, allowing a better control of these diseases.
Palavras-Chave: | HRM analysis; QPCR; Semi quantification.
Thesaurus Nal: | Babesiosis.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Pecuária Sudeste (CPPSE)
Exemplares: | CPPSE - (25345-1) - UPC - AP - DD - PROCI-2021.00078 - GIG - 2021.00085 - DIS