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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
31/01/2012 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, M. R.; GUIMARÃES, M. D. C.; VEIGA, V. M. de O.; MOREIRA, A. dos S.; COSTA, R. R. da; ABI-ZAID, K. C. F.; ROCHA, A. da S.; SUFFYS, P. N. |
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Afiliação: |
MARCIO ROBERTO SILVA, CNPGL; MARK DREW CROSLAND GUIMARÃES, UFMG; VANIA MARIA DE OLIVEIRA VEIGA, CNPGL; ALINE DOS SANTOS MOREIRA, Laboratório de Genômica Funcional e Bioinformática; RONALDO RODRIGUES DA COSTA, UFJF; FHEMIG; KELLY CRISTINA FERREIRA ABI-ZAID, FHEMIG; ADALGIZA DA SILVA ROCHA, FIOCRUZ; PHILIP NOEL SUFFYS, FIOCRUZ. |
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Título: |
Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Memorias do Instituto Oswaldo Cruz, v. 106, n. 1, p. 9-15, 2011. |
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DOI: |
https://doi.org/10.1590/S0074-02762011000100002 |
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Idioma: |
Inglês |
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Conteúdo: |
A cross-sectional analysis of stored Ziehl-Neelsen (ZN)-stained sputum smear slides (SSS) obtained from two public tuberculosis referral laboratories located in Juiz de Fora, Minas Gerais, was carried out to distinguish Mycobacterium bovis from other members of the Mycobacterium tuberculosis complex (MTC). A two-step approach was used to distinguish M. bovis from other members of MTC: (i) oxyR pseudogene amplification to detect MTC and, subsequently, (ii) allele-specific sequencing based on the polymorphism at position 285 of this gene. The oxyR pseudogene was successfully amplified in 100 of 177 (56.5%) SSS available from 99 individuals. No molecular profile of M. bovis was found. Multivariate analysis indicated that acid-fast bacilli (AFB) results and the source laboratory were associated (p < 0.05) with oxyR pseudogene amplification. SSS that were AFB++ SSS showed more oxyR pseudogene amplification than those with AFB0, possibly due to the amount of DNA. One of the two source laboratories presented a greater chance of oxyR pseudogene amplification, suggesting that differences in sputum conservation between laboratories could have influenced the preservation of DNA. This study provides evidence that stored ZN-SSS can be used for the molecular detection of MTC. |
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Palavras-Chave: |
Molecular sequence data; OxyR pseudogene; Sputum smear. |
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Thesagro: |
Mycobacterium Bovis. |
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Thesaurus Nal: |
Mycobacterium tuberculosis; polymerase chain reaction. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53227/1/Artigo-meta-2011-MInstOC-Marcio-2.pdf
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Marc: |
LEADER 02293naa a2200289 a 4500
001 1913965
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S0074-02762011000100002$2DOI
100 1 $aSILVA, M. R.
245 $aIdentification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil.$h[electronic resource]
260 $c2011
520 $aA cross-sectional analysis of stored Ziehl-Neelsen (ZN)-stained sputum smear slides (SSS) obtained from two public tuberculosis referral laboratories located in Juiz de Fora, Minas Gerais, was carried out to distinguish Mycobacterium bovis from other members of the Mycobacterium tuberculosis complex (MTC). A two-step approach was used to distinguish M. bovis from other members of MTC: (i) oxyR pseudogene amplification to detect MTC and, subsequently, (ii) allele-specific sequencing based on the polymorphism at position 285 of this gene. The oxyR pseudogene was successfully amplified in 100 of 177 (56.5%) SSS available from 99 individuals. No molecular profile of M. bovis was found. Multivariate analysis indicated that acid-fast bacilli (AFB) results and the source laboratory were associated (p < 0.05) with oxyR pseudogene amplification. SSS that were AFB++ SSS showed more oxyR pseudogene amplification than those with AFB0, possibly due to the amount of DNA. One of the two source laboratories presented a greater chance of oxyR pseudogene amplification, suggesting that differences in sputum conservation between laboratories could have influenced the preservation of DNA. This study provides evidence that stored ZN-SSS can be used for the molecular detection of MTC.
650 $aMycobacterium tuberculosis
650 $apolymerase chain reaction
650 $aMycobacterium Bovis
653 $aMolecular sequence data
653 $aOxyR pseudogene
653 $aSputum smear
700 1 $aGUIMARÃES, M. D. C.
700 1 $aVEIGA, V. M. de O.
700 1 $aMOREIRA, A. dos S.
700 1 $aCOSTA, R. R. da
700 1 $aABI-ZAID, K. C. F.
700 1 $aROCHA, A. da S.
700 1 $aSUFFYS, P. N.
773 $tMemorias do Instituto Oswaldo Cruz$gv. 106, n. 1, p. 9-15, 2011.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 9 | |
| 1. |  | VEIGA, V. M. de O. Manutenção da saúde da glândula mamária. In: ZOCCAL, R.; ROSA NETO, C.; MOREIRA, P.; SOUZA, V. F. de. Políticas e tecnologias para o leite em Rondônia. Juiz de Fora: Embrapa Gado de Leite, 2010. p. 99-112| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | VEIGA, V. M. de O.; CARNEIRO, A. V.; TEIXEIRA, M. da G. Cuidados com a saúde do rebanho: riscos e consequências. In: CARNEIRO, A. V.; LIMA, I. B. de; MENDES, L. C. R.; RESENDE, M. L. de; ISSA, R. P. N.; LEONEL, F. de P.; MARTINS, P. do C.; TORRES, D. (Ed.). Tecnologias de produção sustentável de bovinos de leite. São João Del Rei: UFSJ, 2010. p. 275-293.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | VEIGA, V. M. de O.; TORRES, R. DE A.; ROCHA, B. B. Enfermidade que ocorrem em vacas leiteiras após o parto por problemas nutricionais. In: Zoccal, R.; Martins, C. E.; Martins, M. C.; Oliveira, L. S. X. de; Novaes, E. A.; Pinto, A. da S. Novos caminhos para o leite em Goiás. Juiz de Fora: Embrapa Gado de Leite, 2009. 213-223| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | SILVA, M. R.; GUIMARÃES, M. D. C.; VEIGA, V. M. de O.; MOREIRA, A. dos S.; COSTA, R. R. da; ABI-ZAID, K. C. F.; ROCHA, A. da S.; SUFFYS, P. N. Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil. Memorias do Instituto Oswaldo Cruz, v. 106, n. 1, p. 9-15, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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