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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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Data corrente: |
11/04/2024 |
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Data da última atualização: |
11/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VAZ, C. S. L.; FONSECA, F. M. da; RECH, D. V.; MORES, M. A. Z.; COLDEBELLA, A.; CANTAO, M. E. |
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Afiliação: |
CLARISSA SILVEIRA LUIZ VAZ, CNPSA; FRANCISCO NOE DA FONSECA, GEM; DAIANE VOSS RECH, CNPSA; MARCOS ANTONIO ZANELLA MORES, CNPSA; ARLEI COLDEBELLA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA. |
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Título: |
Wild-type lytic bacteriophages against Salmonella Heidelberg: further characterization and effect of prophylactic therapy in broiler chickens. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Research in Veterinary Science, v. 171, n. 105247, 2024. |
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DOI: |
https://doi.org/10.1016/j.rvsc.2024.105247 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age. MenosAbstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous a... Mostrar Tudo |
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Palavras-Chave: |
Análise genômica; Bacteriófago; Foodborne pathogen; Genomic analysis; Patógeno de origem alimentar; Terapia fágica. |
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Thesagro: |
Frango de Corte; Salmonelose. |
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Thesaurus Nal: |
Bacteriophages; Phage therapy; Poultry; Salmonellosis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02834naa a2200337 a 4500
001 2163526
005 2024-04-11
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.rvsc.2024.105247$2DOI
100 1 $aVAZ, C. S. L.
245 $aWild-type lytic bacteriophages against Salmonella Heidelberg$bfurther characterization and effect of prophylactic therapy in broiler chickens.$h[electronic resource]
260 $c2024
520 $aAbstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age.
650 $aBacteriophages
650 $aPhage therapy
650 $aPoultry
650 $aSalmonellosis
650 $aFrango de Corte
650 $aSalmonelose
653 $aAnálise genômica
653 $aBacteriófago
653 $aFoodborne pathogen
653 $aGenomic analysis
653 $aPatógeno de origem alimentar
653 $aTerapia fágica
700 1 $aFONSECA, F. M. da
700 1 $aRECH, D. V.
700 1 $aMORES, M. A. Z.
700 1 $aCOLDEBELLA, A.
700 1 $aCANTAO, M. E.
773 $tResearch in Veterinary Science$gv. 171, n. 105247, 2024.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 120 | |
| 2. |  | VAZ, C. S. L. Campylobacter na segurança dos alimentos e na avicultura. Avicultura Industrial, v. 99, n.1165, p.15-19, 2008. Projeto n.16.00.30004-00. Disponível também em: Boletim Pecuário. Disponível em: http://www.boletimpecuario.com.br/notes/noticia.php?not=ancora3051.boletimpecuario| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 3. | | VAZ, C. S. L. Controle biológico de Salmonella enteritidis em aves In: REDE DE INOVAÇÃO TECNOLÓGICA PARA DEFESA AGROPECUÁRIA. Disponível em: http://inovadefesa.ning.com/group/tecnologiasecompetnciasemdefesaagropecuria/forum/topics/controle-biologico-de-1 . Acesso em: 17 Fev. 2010. Projeto/Plano de Ação: 03.08.60.400-02| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 4. | | VAZ, C. S. L. Metodologia para detecção de Campylobacter. In: REDE DE INOVAÇÃO TECNOLÓGICA PARA DEFESA AGROPECUÁRIA. Disponível em: http://inovadefesa.ning.com/group/tecnologiasecompetnciasemdefesaagropecuria/forum/topics/metodologia-para-deteccao-de . Acesso em: 17 Fev. 2010. Projeto/Plano de Ação: 03.08.60.400-02| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 6. | | VAZ, C. S. L. Manejo da cama de frango. In: CONFERÊNCIA FACTA WPSA-BRASIL, 2022, Evento híbrido. Anais: avicultura o futuro é agora. Campinas: FACTA, 2022. Edição digital. Organizado por Ariel Antonio Mendes, Rodrigo Garófallo Garcia, Ibiara C. Almeida Paz, Anselmo Micheletti, Marcelo Zuanaze, Eva Hunka, Silvio Hungaro.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 13. | | CANAL, C. W.; VAZ, C. S. L. Vacinas víricas. In: FLORES, E. F. (Org.). Virologia veterinária. Santa Maria: Ed. da UFSM, 2007. p.329-358. Projeto: 16.00.30004-00| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 14. | | CANAL, C. W.; VAZ, C. S. L. Vacinas víricas. In: FLORES, E. F. (Org.). Virologia veterinária: virologia geral e doenças víricas. 2. ed. Santa Maria: UFSM, 2012. p. 369-398 Projeto/Plano de Ação: 03.08.06.004.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 120 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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