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| Registros recuperados : 34 | |
| 9. |  | BATISTA. P. M.; ANDREOTTI, R.; CHIANG, J. O.; FERREIRA, M. S.; VASCONCELOS, P. F. da C. Seroepidemiological monitoring in sentinel animals and vectors as part of arbovirus surveillance in the State of Mato Grosso do Sul, Brazil Revista da Sociedade Brasileira de Medicina Tropical, v. 45, n. 2, p. 168-173, mar-abr, 2012.| Biblioteca(s): Embrapa Gado de Corte. |
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| 10. | | VASCONCELOS, L. M. R.; VASCONCELOS, P. C. S.; JARDIM, F. C. da S. Efetito do tamanho da clareira na mortalidade de plântula de duas espécies florestais dois anos após o plantio, no município de Moju-Pa. In: SIMPÓSIO LATINO-AMERICANO SOBRE MANEJO FLORESTAL, 2., 2001, Santa Maria. [Anais]. Santa Maria: Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Ciências Florestais, 2001. p. 335-343.| Biblioteca(s): Embrapa Florestas. |
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| 11. | | VASCONCELOS, P.; COSTA, T. A.; SILVA, J. da; BRASILEIRO, A. C.; GIMENES, M. Análise temporal da expressão de resveratrol sintase em genótipos de Arachis. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 4., 2016, Curitiba. Recursos genéticos no Brasil: a base para o desenvolvimento sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2016.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | LOPES, R. M.; SILVEIRA, D.; GIMENES, M. A.; VASCONCELOS, P. S.; COSTA, T. da S. A. Methodology adaptation for the determination of resveratrol in peanut leaves. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 88| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | FREITAS, J. da L.; MALHEIROS, M. A. de B.; VASCONCELOS, P. C. S. Processos fenológicos de Taperebá (Spondias mombin L.) e Pracaxi (Pentaclethra macrolaba (Wild) O. Kuntz) em ecossistema florestal de várzea na Ilha do Pará, Afuá, Pará. Revista de Ciências Agrárias, Belém, PA, n. 39, p.163-172, jan./jun. 2003.| Biblioteca(s): Embrapa Amapá. |
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| 18. | | RODRIGUES, R. C.; VASCONCELOS, P. C. S.; RIBEIRO, M. S.; RIBEIRO, D. B.; BRIEZA JUNIOR, S.; YARED, J. A. G. Classificação de espécies quanto ao hábito utilizadas em trabalhos publicados com sistemas agroflorestais na Amazônia. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 5., 2004, Curitiba. SAFs: desenvolvimento com proteção ambiental: anais. Colombo: Embrapa Florestas, 2004. p. 392-394. (Embrapa Florestas. Documentos, 98).| Biblioteca(s): Embrapa Florestas. |
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| 19. | | RODRIGUES, R. C.; VASCONCELOS, P. C. S.; RIBEIRO, M. S.; RIBEIRO, D. B.; BRIEZA JUNIOR, S.; YARED, J. A. G. Classificação de espécies quanto ao hábito utilizadas em trabalhos publicados com sistemas agroflorestais na Amazônia. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 5., 2004, Curitiba. SAFs: desenvolvimento com proteção ambiental: anais. Colombo: Embrapa Florestas, 2004. p. 392-394. il. (Embrapa Florestas. Documentos, 98). Autoria: BRIEZA [i.e. BRIENZA].| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 20. | | VASCONCELOS, P. C. S.; RODRIGUES, R. C.; RIBEIRO, M. S.; RIBEIRO, D. B.; BRIEZA JUNIOR, S.; YARED, J. A. G. Classificação de trabalhos publicados por tema em sistemas agroflorestais na Amazônia. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 5., 2004, Curitiba. SAFs: desenvolvimento com proteção ambiental: anais. Colombo: Embrapa Florestas, 2004. p. 47-54. (Embrapa Florestas. Documentos, 98).| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 34 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
19/01/2009 |
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Data da última atualização: |
24/07/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KOBAYASHI, A. K.; DIAZ TRUJILLO, C.; LEE, T. van der; ZWIERS, L.-H.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. |
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Afiliação: |
ADILSON KENJI KOBAYASHI, CPAMN; CAUCASELLA DIAZ TRUJILLO, PLANT RESEARCH INTERNATIONAL; THEO VAN DER LEE; LUTE-HARM ZWIERS; LUCIANO V. PAIVA, FEDERAL UNIVERSITY OF LAVRAS; MANOEL TEIXEIRA SOUZA JUNIOR, EMBRAPA LABEX EUROPE; GERT H. J. KEMA, PLANT RESEARCH INTERNATIONAL. |
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Título: |
Generation of transgenic Mycosphaerella fijiensis expressing reporter genes: tools for pathogenicity and mating studies. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Mycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana. MenosMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducte... Mostrar Tudo |
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Palavras-Chave: |
CIRAD139A; CIRAD86; DsRed; GFP. |
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Thesagro: |
Doença; Fungo. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/69938/1/GenerationTransgenic-22676.pdf
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Marc: |
LEADER 02408nam a2200253 a 4500
001 1069938
005 2023-07-24
008 2008 bl uuuu u00u1 u #d
100 1 $aKOBAYASHI, A. K.
245 $aGeneration of transgenic Mycosphaerella fijiensis expressing reporter genes$btools for pathogenicity and mating studies.$h[electronic resource]
260 $aIn: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland$c2008
520 $aMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana.
650 $aDoença
650 $aFungo
653 $aCIRAD139A
653 $aCIRAD86
653 $aDsRed
653 $aGFP
700 1 $aDIAZ TRUJILLO, C.
700 1 $aLEE, T. van der
700 1 $aZWIERS, L.-H.
700 1 $aPAIVA, L. V.
700 1 $aSOUZA JUNIOR, M. T.
700 1 $aKEMA, G. H. J.
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