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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Suínos e Aves. |
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Data corrente: |
23/05/2023 |
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Data da última atualização: |
12/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, T. C. F.; DODE, M. A. N.; BRAGA, T. F.; MARQUES, M. G.; VARGAS, L. N.; FARIA, O. A. C. de; SOUZA, A. P. de; ALBRING, D.; CAETANO, A. R.; FRANCO, M. M. |
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Afiliação: |
THAINARA C. F. SILVA, Universidade Federal de Uberlândia; MARGOT ALVES NUNES DODE, Cenargen; THIAGO F. BRAGA, Centro Universitário do Cerrado; MARIANA GROKE MARQUES, CNPSA; LUNA NASCIMENTO VARGAS, Universidade Federal de Uberlândia; OTÁVIO AUGUSTO COSTA DE FARIA, Universidade de Brasília; ANDRESSA P. DE SOUZA, Universidade do Estado de Santa Catarina; DANIELA ALBRING, Universidade do Contestado; ALEXANDRE RODRIGUES CAETANO, Cenargen; MAURICIO MACHAIM FRANCO, Cenargen. |
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Título: |
Cumulus-oocyte complexes from sows show differences in lipid metabolism compared to cumulus-oocyte complexes from prepubertal gilts during in vitro maturation. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Molecular Reproduction & Development, v. 90, n. 5, p. 323-335. 2023. |
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DOI: |
https://doi.org/10.1002/mrd.23685 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study aimed to evaluate the effects of donor age on lipid metabolism during in
vitro maturation (IVM) of pigs cumulus?oocyte complexes (COCs). We evaluated
transcript levels of genes, the percentage of ooplasm occupied by lipid droplets (LD)
and evaluated DNA methylation in COCs from sows and prepubertal gilts. Transcript
levels of six genes (ACACA, ACSS2, FASN, FABP3, SLC27A4, PLIN2), which were
analyzed in cumulus cells (CCs), increased after 44 h of IVM in the sow group. In the
gilt group, only FASN expression increased, while NR3C1 expression decreased after
IVM. The measurement of LD in oocytes showed an accumulation of lipids in sow
oocytes during IVM, while gilt oocytes showed a decrease in LD. FABP3 and NR3C1
methylation patterns exhibited a demethylation pattern in CCs and oocytes from
gilts and sows and showed statistical differences between groups. CCs from sows
had a better capacity to change transcription levels of the major genes involved in
lipid metabolism during IVM than CCs from gilts. This difference may be involved
in accumulation of lipids, acquisition of competence, and maturation of enclosed
oocytes. Our results contribute to a better understanding of mechanisms involved in
lipid metabolism and acquisition of competence in porcine COCs. |
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Palavras-Chave: |
Donor age; Embryo production; Expressão gênica; Fatty acid; Idade do doador; Pig; Produção de embriões. |
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Thesagro: |
Ácido Graxo; Embrião Animal; Reprodução Animal; Suinocultura. |
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Thesaurus Nal: |
Gene expression. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02474naa a2200385 a 4500
001 2153952
005 2023-06-12
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/mrd.23685$2DOI
100 1 $aSILVA, T. C. F.
245 $aCumulus-oocyte complexes from sows show differences in lipid metabolism compared to cumulus-oocyte complexes from prepubertal gilts during in vitro maturation.$h[electronic resource]
260 $c2023
520 $aAbstract: This study aimed to evaluate the effects of donor age on lipid metabolism during in vitro maturation (IVM) of pigs cumulus?oocyte complexes (COCs). We evaluated transcript levels of genes, the percentage of ooplasm occupied by lipid droplets (LD) and evaluated DNA methylation in COCs from sows and prepubertal gilts. Transcript levels of six genes (ACACA, ACSS2, FASN, FABP3, SLC27A4, PLIN2), which were analyzed in cumulus cells (CCs), increased after 44 h of IVM in the sow group. In the gilt group, only FASN expression increased, while NR3C1 expression decreased after IVM. The measurement of LD in oocytes showed an accumulation of lipids in sow oocytes during IVM, while gilt oocytes showed a decrease in LD. FABP3 and NR3C1 methylation patterns exhibited a demethylation pattern in CCs and oocytes from gilts and sows and showed statistical differences between groups. CCs from sows had a better capacity to change transcription levels of the major genes involved in lipid metabolism during IVM than CCs from gilts. This difference may be involved in accumulation of lipids, acquisition of competence, and maturation of enclosed oocytes. Our results contribute to a better understanding of mechanisms involved in lipid metabolism and acquisition of competence in porcine COCs.
650 $aGene expression
650 $aÁcido Graxo
650 $aEmbrião Animal
650 $aReprodução Animal
650 $aSuinocultura
653 $aDonor age
653 $aEmbryo production
653 $aExpressão gênica
653 $aFatty acid
653 $aIdade do doador
653 $aPig
653 $aProdução de embriões
700 1 $aDODE, M. A. N.
700 1 $aBRAGA, T. F.
700 1 $aMARQUES, M. G.
700 1 $aVARGAS, L. N.
700 1 $aFARIA, O. A. C. de
700 1 $aSOUZA, A. P. de
700 1 $aALBRING, D.
700 1 $aCAETANO, A. R.
700 1 $aFRANCO, M. M.
773 $tMolecular Reproduction & Development$gv. 90, n. 5, p. 323-335. 2023.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
03/02/2017 |
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Data da última atualização: |
03/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
KUWANO, B. H.; NOGUEIRA, M. A.; SANTOS, C. A.; FAGOTTI, D. S. L.; SANTOS, M. B.; LESCANO, L. E. A. M.; ANDRADE, D. S.; BARBOSA, G. M. C.; TAVARES-FILHO, J. |
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Afiliação: |
MARCO ANTONIO NOGUEIRA, CNPSO. |
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Título: |
Application of Landfill Leachate Improves Wheat Nutrition and Yield but Has Minor Effects on Soil Properties. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Environmental Quality, Madison, v. 46, n. 1, p. 153-159, Jan./Feb. 2017. |
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ISSN: |
1537-2537 |
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DOI: |
10.2134/jeq2016.02.0041 |
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Idioma: |
Inglês |
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Conteúdo: |
Landfill leachates, which are potential pollutants, may also carry significant amounts of nutrients that can be recycled by plants. We assessed the nutritional status and yield of wheat (Triticum aestivum L.) and properties of a Rhodic Kandiudult soil (depths of 0–10, 10–20, 20–40, and 40–60 cm) after 11 applications of landfill leachate over 4 yr. In the last application, wheat received 0, 32.7, 65.4, 98.1, or 130.8 m3 ha?1 (875 mg L?1 of nitrogen, N) of leachate and a positive control (90 kg ha?1 of N as urea) 15 d after sowing. Urea increased nitrate (>160 mg kg?1) in the topsoil (down to 40 cm), whereas landfill leachate increased nitrate (>60 mg kg?1) only at 40 to 60 cm with the highest dose, suggesting leaching. Urea-treated soil had less negative ?pH, which might have led to greater retention of nitrate in the topsoil. Sodium (0.02–0.26 cmolc Na kg?1), potassium (0.18–0.82 cmolc K kg?1), and electrical conductivity (0.05–0.14 dS m?1) all increased with leachate dosage. Treatments did not affect resistance to penetration and clay dispersion. Basal respiration increased with leachate dosage, whereas dehydrogenase activity decreased, suggesting effects on soil microbial metabolism. Microbial biomass and soil enzyme activities were not affected by addition of leachate. Nitrogen nutrition (15.1–22.7 g N kg?1 in flag leaves) and grain yield (1381–2378 kg grain ha?1) increased with leachate dosage so that the highest dose gave results similar to those for urea-treated plants (2563 kg grain ha?1). Landfill leachate showed strong potential as source of N for wheat but caused none, or transient, effects on soil properties. However, nitrate from leachate was more leachable than nitrate from urea. MenosLandfill leachates, which are potential pollutants, may also carry significant amounts of nutrients that can be recycled by plants. We assessed the nutritional status and yield of wheat (Triticum aestivum L.) and properties of a Rhodic Kandiudult soil (depths of 0–10, 10–20, 20–40, and 40–60 cm) after 11 applications of landfill leachate over 4 yr. In the last application, wheat received 0, 32.7, 65.4, 98.1, or 130.8 m3 ha?1 (875 mg L?1 of nitrogen, N) of leachate and a positive control (90 kg ha?1 of N as urea) 15 d after sowing. Urea increased nitrate (>160 mg kg?1) in the topsoil (down to 40 cm), whereas landfill leachate increased nitrate (>60 mg kg?1) only at 40 to 60 cm with the highest dose, suggesting leaching. Urea-treated soil had less negative ?pH, which might have led to greater retention of nitrate in the topsoil. Sodium (0.02–0.26 cmolc Na kg?1), potassium (0.18–0.82 cmolc K kg?1), and electrical conductivity (0.05–0.14 dS m?1) all increased with leachate dosage. Treatments did not affect resistance to penetration and clay dispersion. Basal respiration increased with leachate dosage, whereas dehydrogenase activity decreased, suggesting effects on soil microbial metabolism. Microbial biomass and soil enzyme activities were not affected by addition of leachate. Nitrogen nutrition (15.1–22.7 g N kg?1 in flag leaves) and grain yield (1381–2378 kg grain ha?1) increased with leachate dosage so that the highest dose gave results similar to those for urea-treated plant... Mostrar Tudo |
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Thesagro: |
Pedologia; Trigo. |
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Thesaurus NAL: |
Soil properties; Wheat. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02618naa a2200289 a 4500
001 2062630
005 2017-11-03
008 2017 bl uuuu u00u1 u #d
022 $a1537-2537
024 7 $a10.2134/jeq2016.02.0041$2DOI
100 1 $aKUWANO, B. H.
245 $aApplication of Landfill Leachate Improves Wheat Nutrition and Yield but Has Minor Effects on Soil Properties.$h[electronic resource]
260 $c2017
520 $aLandfill leachates, which are potential pollutants, may also carry significant amounts of nutrients that can be recycled by plants. We assessed the nutritional status and yield of wheat (Triticum aestivum L.) and properties of a Rhodic Kandiudult soil (depths of 0–10, 10–20, 20–40, and 40–60 cm) after 11 applications of landfill leachate over 4 yr. In the last application, wheat received 0, 32.7, 65.4, 98.1, or 130.8 m3 ha?1 (875 mg L?1 of nitrogen, N) of leachate and a positive control (90 kg ha?1 of N as urea) 15 d after sowing. Urea increased nitrate (>160 mg kg?1) in the topsoil (down to 40 cm), whereas landfill leachate increased nitrate (>60 mg kg?1) only at 40 to 60 cm with the highest dose, suggesting leaching. Urea-treated soil had less negative ?pH, which might have led to greater retention of nitrate in the topsoil. Sodium (0.02–0.26 cmolc Na kg?1), potassium (0.18–0.82 cmolc K kg?1), and electrical conductivity (0.05–0.14 dS m?1) all increased with leachate dosage. Treatments did not affect resistance to penetration and clay dispersion. Basal respiration increased with leachate dosage, whereas dehydrogenase activity decreased, suggesting effects on soil microbial metabolism. Microbial biomass and soil enzyme activities were not affected by addition of leachate. Nitrogen nutrition (15.1–22.7 g N kg?1 in flag leaves) and grain yield (1381–2378 kg grain ha?1) increased with leachate dosage so that the highest dose gave results similar to those for urea-treated plants (2563 kg grain ha?1). Landfill leachate showed strong potential as source of N for wheat but caused none, or transient, effects on soil properties. However, nitrate from leachate was more leachable than nitrate from urea.
650 $aSoil properties
650 $aWheat
650 $aPedologia
650 $aTrigo
700 1 $aNOGUEIRA, M. A.
700 1 $aSANTOS, C. A.
700 1 $aFAGOTTI, D. S. L.
700 1 $aSANTOS, M. B.
700 1 $aLESCANO, L. E. A. M.
700 1 $aANDRADE, D. S.
700 1 $aBARBOSA, G. M. C.
700 1 $aTAVARES-FILHO, J.
773 $tJournal of Environmental Quality, Madison$gv. 46, n. 1, p. 153-159, Jan./Feb. 2017.
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