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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/11/2010 |
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Data da última atualização: |
22/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, K. A.; ALMEIDA, T. C.; GESTEIRA, A. da S.; LÔBO, I. P.; GUIMARÃES, A. C. R.; MIRANDA, A. B. de; VAN SLUYS, M. A.; CRUZ, R. S. da; CASCARDO, J. C. M.; CARELS, N. |
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Afiliação: |
Kleber A. Gomes, UESC; Tiago C. Almeida, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; Ivon Pinheiro Lobo, UESC; Ana Carolina R. Guimarães, FIOCRUZ; Antonio B. de Miranda, FIOCRUZ; Marie-Anne Van Sluys, USP; Rosenira Serpa da Cruz, UESC; Júlio Cézar Mattos Cascardo, UESC; Nicolas Carels, UESC. |
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Título: |
ESTs from seeds to assist the selective breeding of Jatropha curcas L. for oil and active compounds. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Genomics Insights, v. 3, p. 29?56, 2010. |
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ISSN: |
1178-6310 |
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DOI: |
10.4137/GEI.S4340 |
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Idioma: |
Inglês |
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Notas: |
Diponível em:.Acesso em: 03 nov. 2010. |
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Conteúdo: |
We report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality $10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes (palmitoyl-acyl carrier protein thioesterase, 3-ketoacyl-CoA thiolase B, lysophosphatidic acid acyltransferase and geranyl pyrophosphate synthase) measured by real- time PCR proved to be significantly different between leaves and fruits. Since the nucleotide polymorphism of these tags is associated to higher level of gene expression in fruits compared to leaves, we propose this approach to speed up the search for quantitative traits in selective breeding of J. curcas. We also discuss its potential utility for the selective breeding of economically important traits in J. curcas. |
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Palavras-Chave: |
Biofuel; Melhoramento genetico vegatal; Pinhão manso; Terpenes. |
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Thesagro: |
Biocombustível; Jatropha Curcas. |
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Thesaurus Nal: |
alkaloids; fatty acids; genomics. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/23536/1/f-2328-GEI-ESTs-from-Seeds-to-Assist-the-Selective-Breeding-of-Jatropha-curcas-L.pdf-31711.pdf
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Marc: |
LEADER 02518naa a2200373 a 4500
001 1865928
005 2022-08-22
008 2010 bl uuuu u00u1 u #d
022 $a1178-6310
024 7 $a10.4137/GEI.S4340$2DOI
100 1 $aGOMES, K. A.
245 $aESTs from seeds to assist the selective breeding of Jatropha curcas L. for oil and active compounds.$h[electronic resource]
260 $c2010
500 $aDiponível em:<http://www.la-press.com/ests-from-seeds-to-assist-the-selective-breeding-of-jatropha-curcas-l--article-a2328>.Acesso em: 03 nov. 2010.
520 $aWe report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality $10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes (palmitoyl-acyl carrier protein thioesterase, 3-ketoacyl-CoA thiolase B, lysophosphatidic acid acyltransferase and geranyl pyrophosphate synthase) measured by real- time PCR proved to be significantly different between leaves and fruits. Since the nucleotide polymorphism of these tags is associated to higher level of gene expression in fruits compared to leaves, we propose this approach to speed up the search for quantitative traits in selective breeding of J. curcas. We also discuss its potential utility for the selective breeding of economically important traits in J. curcas.
650 $aalkaloids
650 $afatty acids
650 $agenomics
650 $aBiocombustível
650 $aJatropha Curcas
653 $aBiofuel
653 $aMelhoramento genetico vegatal
653 $aPinhão manso
653 $aTerpenes
700 1 $aALMEIDA, T. C.
700 1 $aGESTEIRA, A. da S.
700 1 $aLÔBO, I. P.
700 1 $aGUIMARÃES, A. C. R.
700 1 $aMIRANDA, A. B. de
700 1 $aVAN SLUYS, M. A.
700 1 $aCRUZ, R. S. da
700 1 $aCASCARDO, J. C. M.
700 1 $aCARELS, N.
773 $tGenomics Insights$gv. 3, p. 29?56, 2010.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
09/08/2010 |
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Data da última atualização: |
09/08/2010 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
QUIRINO, B. F.; CUNHA, I. S.; SOUTO, B. de M.; CARVALHO, L. S.; RAMOS, N. S. P. L.; COSTA, O. Y. A.; BARRETO, C. C.; KRUGER, R. H. |
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Afiliação: |
BETANIA FERRAZ QUIRINO, CNPAE; ISABEL S. CUNHA, UCB; BETULIA DE MORAIS SOUTO, CNPAE; LUCAS S. CARVALHO; NIDIA S. P. L. RAMOS; OHANA Y. A. COSTA, UCB; CRISTINE C. BARRETO, UCB; RICARDO H. KRUGER, UnB. |
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Título: |
Characterization of the bacterial diversity in the goat rumen and identification of enzymes with potential use in the biofuels industry. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: ANNUAL MEETING AND EXHIBITION, 60., 2010, San Francisco, CA. Industrial microbiology and biotechnology. [S.l]: Society for Industrial Microbiology, 2010. |
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Páginas: |
p. 107 |
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Idioma: |
Português |
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Notas: |
Poster abstract P9. |
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Palavras-Chave: |
Rúmen de caprino. |
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Thesagro: |
Biocombustível; Enzima. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00870naa a2200253 a 4500
001 1859637
005 2010-08-09
008 2010 bl --- 0-- u #d
100 1 $aQUIRINO, B. F.
245 $aCharacterization of the bacterial diversity in the goat rumen and identification of enzymes with potential use in the biofuels industry.
260 $c2010
300 $ap. 107
500 $aPoster abstract P9.
650 $aBiocombustível
650 $aEnzima
653 $aRúmen de caprino
700 1 $aCUNHA, I. S.
700 1 $aSOUTO, B. de M.
700 1 $aCARVALHO, L. S.
700 1 $aRAMOS, N. S. P. L.
700 1 $aCOSTA, O. Y. A.
700 1 $aBARRETO, C. C.
700 1 $aKRUGER, R. H.
773 $tIn: ANNUAL MEETING AND EXHIBITION, 60., 2010, San Francisco, CA. Industrial microbiology and biotechnology. [S.l]: Society for Industrial Microbiology, 2010.
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Embrapa Agroenergia (CNPAE) |
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