Data/hora: 13/06/2024 00:36:02

Biblioteca(s): 	Embrapa Milho e Sorgo.	
Data corrente: 	05/03/2024	
Data da última atualização: 	10/05/2024	
Autoria: 	NASCIMENTO, T. A.; CARVALHO, K. S. de; MARTINS, D. C.; OLIVEIRA, C. R. de; QUEIROZ, M. M. de C.; VALICENTE, F. H.	
Afiliação: 	TATIANE APARECIDA NASCIMENTO, UNIVERSIDADE FEDERAL DE LAVRAS; KARINE SILVA DE CARVALHO, UNIVERSIDADE FEDERAL DE LAVRAS; DENIZE CARVALHO MARTINS; CLEIDIANE RODRIGUES DE OLIVEIRA; MARGARETH MARIA DE CARVALHO QUEIROZ, FUNDAÇÃO OSWALDO CRUZ; FERNANDO HERCOS VALICENTE, CNPMS.	
Título: 	Bacillus thuringiensis strains to control Noctuidae pests.	
Ano de publicação: 	2024	
Fonte/Imprenta: 	Journal of Applied Entomology, v. 148, p. 371-381, 2024.	
DOI: 	http://doi.org/10.1111/jen.13230	
Idioma: 	Inglês	
Conteúdo: 	This study aimed to select and characterize Bacillus thuringiensis strains with toxic activity on Helicoverpa armigera, Spodoptera cosmioides and Spodoptera eridania. Fifty B. thuringiensis (Bt) strains were used in selective bioassays for high mortality on H. armigera, and the four most efficient strains were tested on S. cosmioides and S. eridania. Twelve Bt strains were characterized by PCR to detect some cry, cyt and vip genes. Bioassays were also performed to detect the production of beta-exotoxins. Strain 237 caused a mortality rate of 95.6% due to beta-exotoxins. Strains 1368, 1521, 1814 and 3299 showed toxic activity on S. eridania. However, no strain was effective on S. cosmioides. The presence of beta-exotoxin was detected only in strain 237. The molecular characterization of the strains using specific cry, cyt and vip primers showed a great genetic diversity among the 12 strains that showed some mortality on H. armigera and S. eridania. Our results showed that detecting beta-exotoxin in Bt strains is important as it is toxic to insects and vertebrates and persistent in the environment. Strains 1368, 1521, 1814 and 3299 did not show toxicity to H. armigera, while they were toxic to S. eridania and did not produce beta-exotoxin. These strains may be useful for new formulations of biopesticides as well as for cloning and expression of plants' genes.	
Palavras-Chave: 	Bactéria entomopatogênica; Bioensaio; Gene inseticida; Insecticidal gene; Microbial control.	
Thesagro: 	Bactéria; Controle Microbiano.	
Thesaurus Nal: 	Bioassays; Entomopathogenic bacteria; Lepidoptera.	
Categoria do assunto: 	S Ciências Biológicas	
Registro original: 	Embrapa Milho e Sorgo (CNPMS)	
Exemplares: 	CNPMS - (30288-1) - UPC - AP - DD -  -  -  - DIS