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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
09/12/2019 |
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Data da última atualização: |
13/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROJAS-LORZ, L.; ARRIETA-ESPINOZA, G.; VALDEZ-MELARA, M.; PEREIRA, L. F. P.; GATICA-ARIAS, A. |
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Afiliação: |
Laura Rojas-Lorz, University of Costa Rica/School of Biology/Laboratory of Plant Biotechnology; Griselda Arrieta-Espinoza, University of Costa Rica/Research Center in Cellular and Molecular Biology - CIBCM; Marta Valdez-Melara, University of Costa Rica/School of Biology/Laboratory of Plant Biotechnology; LUIZ FILIPE PROTASIO PEREIRA, CNPCa; Andrés Gatica-Arias, University of Costa Rica/School of Biology/Laboratory of Plant Biotechnology. |
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Título: |
Influence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L.). |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Brazilian Archives of Biology and Technology, v. 62, e19180228, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Plant somatic embryogenesis (SE) has been defined as the formation of embryos from a single or group of haploid or somatic cells [1, 2]. Low frequency (LFSE) and high frequency somatic embryogenesis (HFSE) have been described. In the first type, somatic embryos are induced directly from pro-embryogenic cells of explants, while in the second, they originate from embryogenic callus [1]. It has been suggested that in LFSE the origin of somatic embryos is unicellular, whereas in HFSE has been described as unicellular or multicellular [3]. SE is a powerful biotechnological tool used to propagate elite plants or to conserve important genotypes [4]. Moreover, SE offers an efficient in vitro regeneration approach as a fundamental step in plant genetic improvement for studying basic aspects of ontogenesis of somatic embryos [5]. In Coffea spp., the first studies of SE have been reported at the beginning of 1970 [6]. Since then, a large quantity of LFSE and HFSE protocols have been optimized demonstrating that coffee is not a recalcitrant species for SE [4]. In the LFSE the somatic embryos are obtained faster (approximately 70 days) using only one medium meanwhile in HFSE several media are used and somatic embryo formation takes 9-10 months [4]. Although, in LFSE small number of somatic embryos are obtained (around 10 per explant) compared to hundreds of somatic embryos obtained per gram of embryogenic calli [4], the unicellular origin of somatic embryos in LFSE represents an advantage for the chemical and physical mutagenesis, genetic transformation and genetic editing, since prevents or reduces the appearance of chimeras [7]. In C. arabica and C. canephora many factors (such as genotype, explant type, the physiological state, age and growth conditions of the donor plants, the season of collection, nutrient composition of the medium, the volume of dissolved CO2 or O2 in the culture flask, and plant growth regulators) that affect LFSE induction have been studied [3, 8, 9, 10, 11, 12, 13]. However, few studies reported the effect of silver nitrate on LFSE using leaf explants of C. arabica L. and to the best of our knowledge it has not been analyzed using Caturra and Catuaí, which are two economic important producer cultivars in Costa Rica. Since SE is genotype dependent, the culture medium need to be modified for the different genotypes [7].Therefore, the objective of this study was to determine the influence of the benzyladenine (BAP), indole-3-acetic acid (IAA), and silver nitrate (AgNO3) on low frequency somatic embryogenesis using leaf explants of Coffea arabica L. cultivars Caturra and Catuaí. MenosPlant somatic embryogenesis (SE) has been defined as the formation of embryos from a single or group of haploid or somatic cells [1, 2]. Low frequency (LFSE) and high frequency somatic embryogenesis (HFSE) have been described. In the first type, somatic embryos are induced directly from pro-embryogenic cells of explants, while in the second, they originate from embryogenic callus [1]. It has been suggested that in LFSE the origin of somatic embryos is unicellular, whereas in HFSE has been described as unicellular or multicellular [3]. SE is a powerful biotechnological tool used to propagate elite plants or to conserve important genotypes [4]. Moreover, SE offers an efficient in vitro regeneration approach as a fundamental step in plant genetic improvement for studying basic aspects of ontogenesis of somatic embryos [5]. In Coffea spp., the first studies of SE have been reported at the beginning of 1970 [6]. Since then, a large quantity of LFSE and HFSE protocols have been optimized demonstrating that coffee is not a recalcitrant species for SE [4]. In the LFSE the somatic embryos are obtained faster (approximately 70 days) using only one medium meanwhile in HFSE several media are used and somatic embryo formation takes 9-10 months [4]. Although, in LFSE small number of somatic embryos are obtained (around 10 per explant) compared to hundreds of somatic embryos obtained per gram of embryogenic calli [4], the unicellular origin of somatic embryos in LFSE represents an advantag... Mostrar Tudo |
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Palavras-Chave: |
Plant growth regulators. |
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Thesaurus Nal: |
Silver nitrate; Somatic embryos; Tissue culture. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/206428/1/Influence-of-silver-nitrate-on-somatic.pdf
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Marc: |
LEADER 03326naa a2200217 a 4500
001 2116284
005 2019-12-13
008 2019 bl uuuu u00u1 u #d
100 1 $aROJAS-LORZ, L.
245 $aInfluence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L.).$h[electronic resource]
260 $c2019
520 $aPlant somatic embryogenesis (SE) has been defined as the formation of embryos from a single or group of haploid or somatic cells [1, 2]. Low frequency (LFSE) and high frequency somatic embryogenesis (HFSE) have been described. In the first type, somatic embryos are induced directly from pro-embryogenic cells of explants, while in the second, they originate from embryogenic callus [1]. It has been suggested that in LFSE the origin of somatic embryos is unicellular, whereas in HFSE has been described as unicellular or multicellular [3]. SE is a powerful biotechnological tool used to propagate elite plants or to conserve important genotypes [4]. Moreover, SE offers an efficient in vitro regeneration approach as a fundamental step in plant genetic improvement for studying basic aspects of ontogenesis of somatic embryos [5]. In Coffea spp., the first studies of SE have been reported at the beginning of 1970 [6]. Since then, a large quantity of LFSE and HFSE protocols have been optimized demonstrating that coffee is not a recalcitrant species for SE [4]. In the LFSE the somatic embryos are obtained faster (approximately 70 days) using only one medium meanwhile in HFSE several media are used and somatic embryo formation takes 9-10 months [4]. Although, in LFSE small number of somatic embryos are obtained (around 10 per explant) compared to hundreds of somatic embryos obtained per gram of embryogenic calli [4], the unicellular origin of somatic embryos in LFSE represents an advantage for the chemical and physical mutagenesis, genetic transformation and genetic editing, since prevents or reduces the appearance of chimeras [7]. In C. arabica and C. canephora many factors (such as genotype, explant type, the physiological state, age and growth conditions of the donor plants, the season of collection, nutrient composition of the medium, the volume of dissolved CO2 or O2 in the culture flask, and plant growth regulators) that affect LFSE induction have been studied [3, 8, 9, 10, 11, 12, 13]. However, few studies reported the effect of silver nitrate on LFSE using leaf explants of C. arabica L. and to the best of our knowledge it has not been analyzed using Caturra and Catuaí, which are two economic important producer cultivars in Costa Rica. Since SE is genotype dependent, the culture medium need to be modified for the different genotypes [7].Therefore, the objective of this study was to determine the influence of the benzyladenine (BAP), indole-3-acetic acid (IAA), and silver nitrate (AgNO3) on low frequency somatic embryogenesis using leaf explants of Coffea arabica L. cultivars Caturra and Catuaí.
650 $aSilver nitrate
650 $aSomatic embryos
650 $aTissue culture
653 $aPlant growth regulators
700 1 $aARRIETA-ESPINOZA, G.
700 1 $aVALDEZ-MELARA, M.
700 1 $aPEREIRA, L. F. P.
700 1 $aGATICA-ARIAS, A.
773 $tBrazilian Archives of Biology and Technology$gv. 62, e19180228, 2019.
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Registro original: |
Embrapa Café (CNPCa) |
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| 2. |  | PEREIRA, L. F. P.; VILLALTA-VILLALOBOS, J.; ROJAS-LORZ, L.; VALDEZ-MELARA, M. F.; ARRIETA-ESPINOZA, G.; GATICA-ARIAS, A. Coffea arabica L. biotechnological improvement against coffee berry borer (CBB). In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 40| Tipo: Resumo em Anais de Congresso |
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