Data/hora: 02/06/2024 12:55:00

Biblioteca(s): 	Embrapa Agrobiologia.	
Data corrente: 	30/07/2001	
Data da última atualização: 	30/07/2001	
Autoria: 	TRUJILLO, L. E.; ARRIETA, J. G.; DAFHNIS, F.; GARCIA, J.; VALDES, J.; TAMBARA, Y.; PEREZ, M.; HERNANDEZ, L.	
Título: 	Fructo-oligosaccharides production by the Gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast Pichia pastoris.	
Ano de publicação: 	2001	
Fonte/Imprenta: 	Enzyme and Microbial Technology, v.28, p.139-144, 2001.	
Idioma: 	Inglês	
Conteúdo: 	Levansucrase (LsdA) (EC 2.4.1.10) from Gluconacetobacter diazotrophicus (formerly Acetobacter diazotrophicus) yields high levels of fructo-oligosaccharides (FOS) from sucrose. A DNA fragment encoding the precursor LsdA lacking the first 57 amino acids was fused to the phol signal sequence under the control of the Pichia pastoris-alcohol oxidase I (AOXI) promoter. Methanol induction of a P. pastoris strain harboring a single copy of the lsdA expression cassette integrated in the genome resulted in the production of active levansucrase. After fermentation of the recombinant yeast, LsdA activity was detected in the periplasmic fraction (81%) and in the culture supernatant (18%) with an overall yield of 1% of total protein. The recombinant LsdA was glycosylated and displayed optimal pH and temperature for enzyme activity similar to those of the native enzyme, but thermal stability was increased. Neither  	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agrobiologia (CNPAB)	
Exemplares: 	CNPAB - (24629-1) - ADD - SP - -- -  -  - 22438 - DIS