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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/02/1996 |
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Data da última atualização: |
26/02/1996 |
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Autoria: |
GIRAUDO CONESA, L. C.; VANNELLI, S. A.; UZAL, F. A. |
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Título: |
Detection of clostridium chauvooei in formalin-fixed, paraffin-embedded tissues of sheep by the peroxidase-antiperoxidase (PAP) tecnique. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Veterinary Research Communications, v.19, n.6, p.451-456, 1995. |
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Idioma: |
Inglês |
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Conteúdo: |
A peroxidase-antiperoxidase (PAP) technique was used to detect Clostridium chauvoei in tissue sections from sheep inoculated intramuscularly with a pure culture of this microorganism. Samples of various tissues were taken for bacteriology, histopathology and immunohistochemistry. A primary antiserum against C. chauvoei for use in the PAP technique was produced in rabbits. Formalin-fixed, paraffin-embedded sections of muscle samples were positively and specifically stained by the PAP technique. The results were consistent with those obtained by bacteriology, but the PAP test was simple, quicker and less expensive than the bacteriological procedures. The use of the PAP technique would be appropriate for detecting clostridial infections without the constraints of conventional identification methods, especially where laboratory conditions for anaerobic procedures are not readily available.
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Palavras-Chave: |
Clostridlose; Hicrobiologia; PAP. |
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Thesagro: |
Diagnostico. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01497naa a2200193 a 4500
001 1516572
005 1996-02-26
008 1995 bl uuuu u00u1 u #d
100 1 $aGIRAUDO CONESA, L. C.
245 $aDetection of clostridium chauvooei in formalin-fixed, paraffin-embedded tissues of sheep by the peroxidase-antiperoxidase (PAP) tecnique.
260 $c1995
520 $aA peroxidase-antiperoxidase (PAP) technique was used to detect Clostridium chauvoei in tissue sections from sheep inoculated intramuscularly with a pure culture of this microorganism. Samples of various tissues were taken for bacteriology, histopathology and immunohistochemistry. A primary antiserum against C. chauvoei for use in the PAP technique was produced in rabbits. Formalin-fixed, paraffin-embedded sections of muscle samples were positively and specifically stained by the PAP technique. The results were consistent with those obtained by bacteriology, but the PAP test was simple, quicker and less expensive than the bacteriological procedures. The use of the PAP technique would be appropriate for detecting clostridial infections without the constraints of conventional identification methods, especially where laboratory conditions for anaerobic procedures are not readily available.
650 $aDiagnostico
653 $aClostridlose
653 $aHicrobiologia
653 $aPAP
700 1 $aVANNELLI, S. A.
700 1 $aUZAL, F. A.
773 $tVeterinary Research Communications$gv.19, n.6, p.451-456, 1995.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 4 | |
| 1. |  | ASSIS, R. A. de; LOBATO, F. C. F.; MIYAKAWA, M. F.; UZAL, F. A. Reação em cadeia da polimerase para detecção de Clostridium chauvoei em tecidos de Cavia porcellus. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 12, p. 1247-1250, dez. 2005 Notas Científicas.
Título em inglês: PCR detection of Clostridium chauvoei in tissues of Cavia porcellus.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | SCHILD, A. L.; FISS, L.; DAME, M. C.; UZAL, F. A.; SOARES, M. P.; SCHUCH, L. F.; FLORES, E. F.; RIET-CORREA, F. Congenital hydranencephaly and cerebellar hypoplasia in water buffalo in southern Brazil. Journal of Veterinary Diagnostic Investigation, v. 23, n. 3, p. 603-609, May 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 3. | | VESCHI, J. L. A.; DUTRA, I. S.; ALVES, M. A. B.; FERNANDEZ-MIYAKAWA, M. E.; PERRI, S. H. V.; UZAL, F. A. Sorological evaluation of commercial vaccines against enterotoxemia in goats. In: INTERNATIONAL CONFERENCE ON GOATS, 9.; REUNIÓN NACIONAL SOBRE CAPRINOCULTURA, 23, 2008, Querétaro, México. Sustainable goat production: challenges an opportunities of small and large enterprises; proceedings. Querétaro: International Goat Association, 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Semiárido. |
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| 4. | | FRADE, M. T. S.; MELO, L. F. de; PESSOA, C. R. M.; ARAÚJO, J. L. de; FIGHERA, R. A.; SOUZA, A. P.; UZAL, F.; DANTAS, A. F. M. Systemic acanthamoebiasis associated with canine distemper in dogs in the semiarid region of Paraíba, Brazil. Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 2, p. 160-164, fev. 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 4 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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