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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
17/07/2003 |
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Data da última atualização: |
28/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRITO, L. F. C.; SILVA, A. E. D. F.; BARBOSA, R. T.; UNANIAN, M. M.; KASTELIC, J. P. |
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Afiliação: |
LEONARDO F. C. BRITO; ANTONIO EMIDIO DIAS FELICIANO SILVA, CPPSE, SÃO CARLOS, SP.; ROGERIO TAVEIRA BARBOSA, CPPSE; MARIA MARINA UNANIAN, CPPSE, SÃO CARLOS, SP.; JOHN P. KASTELIC. |
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Título: |
Effects of scrotal insulation on sperm production semen quality and testicular echotexture in bos indicus and Bos indicus x Bos taurus bulls. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 79, n. 1, p. 1-15, 2003. |
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DOI: |
10.1016/S0378-4320(03)00082-4 |
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Idioma: |
Inglês |
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Conteúdo: |
The objectives of the present study were to evaluate the effects of scrotal insulation on sperm production, semen quality, and testicular echotexture in Bos indicus and Bos indicus × Bos taurus crossbred bulls. In one experiment, B. indicus bulls (n=12) were allocated to control and whole-scrotum insulation groups, while in a second experiment, crossbred bulls (n=21) were allocated into control, whole-scrotum, and scrotal-neck insulation groups. Insulation was applied for 4 days (start of insulation = Day 0) and semen collection and testicular ultrasonographic examinations were performed twice weekly until Day 35. Sperm concentration and total sperm output during the post-insulation period were greater in control groups, but significant differences were observed only in B. indicus bulls. Overall, sperm motility in scrotal-insulated B. indicus bulls was lower (P<0.05) than in the control group. After whole-scrotum insulation in crossbred bulls, sperm motility was lower (P<0.05) than pre-insulation levels between Days 21 and 31, and lower than control levels on Day 24. The proportion of normal sperm after whole-scrotum insulation was lower than pre-insulation and control values from Day 11 to the end of the experiment in B. indicus bulls (P<0.05 from Days 14 to 21 and on Day 27), and from Days 14 to 25 in crossbred bulls (P<0.05 on Days 14 and 18). Insulation of the scrotal neck in crossbred bulls did not significantly affect semen quality. Loose sperm heads (Day 11), midpiece defects (Days 11 and 14), and acrosome defects (Days 27 and 31) increased (P<0.05) in insulated B. indicus bulls, while proximal cytoplasmic droplets (Days 14, 18 and 27 in B. indicus; Days 24 and 27 in crossbred bulls) and sperm vacuoles (Days 18 and 21 in B. indicus; Day 18 in crossbred bulls) increased (P<0.05) in whole-scrotum insulation groups in both experiments. There was considerable variation among bulls in the incidence of specific sperm defects. The timing of appearance of sperm defects after insulation provided insights into the pathogenesis of specific abnormalities. Neither whole-scrotum nor scrotal-neck insulation affected testicular echotexture in either experiment. In conclusion, whole-scrotum insulation resulted in decreased sperm production and semen quality in B. indicus and B. indicus × B. taurus bulls, but those changes were not associated with changes in testicular echotexture. MenosThe objectives of the present study were to evaluate the effects of scrotal insulation on sperm production, semen quality, and testicular echotexture in Bos indicus and Bos indicus × Bos taurus crossbred bulls. In one experiment, B. indicus bulls (n=12) were allocated to control and whole-scrotum insulation groups, while in a second experiment, crossbred bulls (n=21) were allocated into control, whole-scrotum, and scrotal-neck insulation groups. Insulation was applied for 4 days (start of insulation = Day 0) and semen collection and testicular ultrasonographic examinations were performed twice weekly until Day 35. Sperm concentration and total sperm output during the post-insulation period were greater in control groups, but significant differences were observed only in B. indicus bulls. Overall, sperm motility in scrotal-insulated B. indicus bulls was lower (P<0.05) than in the control group. After whole-scrotum insulation in crossbred bulls, sperm motility was lower (P<0.05) than pre-insulation levels between Days 21 and 31, and lower than control levels on Day 24. The proportion of normal sperm after whole-scrotum insulation was lower than pre-insulation and control values from Day 11 to the end of the experiment in B. indicus bulls (P<0.05 from Days 14 to 21 and on Day 27), and from Days 14 to 25 in crossbred bulls (P<0.05 on Days 14 and 18). Insulation of the scrotal neck in crossbred bulls did not significantly affect semen quality. Loose sperm heads (Day 11), midpiece... Mostrar Tudo |
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Palavras-Chave: |
Bull; Scrotal insulation; Sperm; Ultrasound. |
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Thesagro: |
Sêmen. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03181naa a2200241 a 4500
001 1046442
005 2023-06-28
008 2003 bl uuuu u00u1 u #d
024 7 $a10.1016/S0378-4320(03)00082-4$2DOI
100 1 $aBRITO, L. F. C.
245 $aEffects of scrotal insulation on sperm production semen quality and testicular echotexture in bos indicus and Bos indicus x Bos taurus bulls.$h[electronic resource]
260 $c2003
520 $aThe objectives of the present study were to evaluate the effects of scrotal insulation on sperm production, semen quality, and testicular echotexture in Bos indicus and Bos indicus × Bos taurus crossbred bulls. In one experiment, B. indicus bulls (n=12) were allocated to control and whole-scrotum insulation groups, while in a second experiment, crossbred bulls (n=21) were allocated into control, whole-scrotum, and scrotal-neck insulation groups. Insulation was applied for 4 days (start of insulation = Day 0) and semen collection and testicular ultrasonographic examinations were performed twice weekly until Day 35. Sperm concentration and total sperm output during the post-insulation period were greater in control groups, but significant differences were observed only in B. indicus bulls. Overall, sperm motility in scrotal-insulated B. indicus bulls was lower (P<0.05) than in the control group. After whole-scrotum insulation in crossbred bulls, sperm motility was lower (P<0.05) than pre-insulation levels between Days 21 and 31, and lower than control levels on Day 24. The proportion of normal sperm after whole-scrotum insulation was lower than pre-insulation and control values from Day 11 to the end of the experiment in B. indicus bulls (P<0.05 from Days 14 to 21 and on Day 27), and from Days 14 to 25 in crossbred bulls (P<0.05 on Days 14 and 18). Insulation of the scrotal neck in crossbred bulls did not significantly affect semen quality. Loose sperm heads (Day 11), midpiece defects (Days 11 and 14), and acrosome defects (Days 27 and 31) increased (P<0.05) in insulated B. indicus bulls, while proximal cytoplasmic droplets (Days 14, 18 and 27 in B. indicus; Days 24 and 27 in crossbred bulls) and sperm vacuoles (Days 18 and 21 in B. indicus; Day 18 in crossbred bulls) increased (P<0.05) in whole-scrotum insulation groups in both experiments. There was considerable variation among bulls in the incidence of specific sperm defects. The timing of appearance of sperm defects after insulation provided insights into the pathogenesis of specific abnormalities. Neither whole-scrotum nor scrotal-neck insulation affected testicular echotexture in either experiment. In conclusion, whole-scrotum insulation resulted in decreased sperm production and semen quality in B. indicus and B. indicus × B. taurus bulls, but those changes were not associated with changes in testicular echotexture.
650 $aSêmen
653 $aBull
653 $aScrotal insulation
653 $aSperm
653 $aUltrasound
700 1 $aSILVA, A. E. D. F.
700 1 $aBARBOSA, R. T.
700 1 $aUNANIAN, M. M.
700 1 $aKASTELIC, J. P.
773 $tAnimal Reproduction Science$gv. 79, n. 1, p. 1-15, 2003.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
12/03/2008 |
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Data da última atualização: |
05/04/2023 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
GOUVEIA, J. J. de S.; REGITANO, L. C. de A. |
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Afiliação: |
João José de Simoni, UFSCar/SÃO CARLOS, SP.; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
Extração de DNA. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: REGITANO, L. C. de A.; NICIURA, S. C. M.; IBELLI, A. M. G.; GOUVEIA, J. J. de S. Protocolos em biologia molecular aplicada à produção animal. São Carlos: Embrapa Pecuária Sudeste, 2007. |
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Páginas: |
p. 3-8. |
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Idioma: |
Português |
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Conteúdo: |
A extração dos ácidos nucléicos (DNA e RNA) é o primeiro passo para a realização da maioria das metodologias de biologia molecular. |
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Thesagro: |
Biologia Molecular; DNA; Extração. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPPSE/17536/1/PROCILCAR2007.00415.pdf
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Marc: |
LEADER 00748naa a2200181 a 4500
001 1048302
005 2023-04-05
008 2007 bl uuuu u00u1 u #d
100 1 $aGOUVEIA, J. J. de S.
245 $aExtração de DNA.$h[electronic resource]
260 $c2007
300 $ap. 3-8.
520 $aA extração dos ácidos nucléicos (DNA e RNA) é o primeiro passo para a realização da maioria das metodologias de biologia molecular.
650 $aBiologia Molecular
650 $aDNA
650 $aExtração
700 1 $aREGITANO, L. C. de A.
773 $tIn: REGITANO, L. C. de A.; NICIURA, S. C. M.; IBELLI, A. M. G.; GOUVEIA, J. J. de S. Protocolos em biologia molecular aplicada à produção animal. São Carlos: Embrapa Pecuária Sudeste, 2007.
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