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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/08/1999 |
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Data da última atualização: |
12/08/1999 |
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Autoria: |
CARLSON, J. E.; TULSIERAM, L. K.; GLAUBITZ, J. C.; LUK, V. W. K.; KAUFFELDT, C.; RUTLEDGE, R. |
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Afiliação: |
The Biotechnology Laboratory and the Faculty of Forestry at the University of British Columbia; Forestry Canada, Petawawa National Forestry Institute, Chalk River, Ontario, Canada KOJ 1JO. |
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Título: |
Segregation of random amplified DNA markers in F1 progny of conifers |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Theor Appl Genet, v.83, p.194-200, 1991 |
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Idioma: |
Inglês |
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Conteúdo: |
The recently developed approach to deriving genetic markers via amplification of random DNA segments with single primers of arbitrary nucleotide sequence was tested for its utility in genetic linkage mapping studies with conifers. Reaction conditions were optimized to reproducibly yield clean and specific amplification products. Template DNA from several genotypes of Douglas-fir (Pseudotsuga menziesii) and white spruce (Picea glauca) were tested against eight ten-base oligonucleotide primers. Most of the tested primer/parent tree combinations yielded polymorphic PCR products ("RAPD" markers). Selected primers were then used in PCR reactions with template DNA isolated from offspring in Douglas-fir and black spruce diallel crosses among the same parental lines. |
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Palavras-Chave: |
Conifers-Genetic-Markers; Linkage mapping; Random amplified DNA markers. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01373naa a2200217 a 4500
001 1648244
005 1999-08-12
008 1991 bl --- 0-- u #d
100 1 $aCARLSON, J. E.
245 $aSegregation of random amplified DNA markers in F1 progny of conifers
260 $c1991
520 $aThe recently developed approach to deriving genetic markers via amplification of random DNA segments with single primers of arbitrary nucleotide sequence was tested for its utility in genetic linkage mapping studies with conifers. Reaction conditions were optimized to reproducibly yield clean and specific amplification products. Template DNA from several genotypes of Douglas-fir (Pseudotsuga menziesii) and white spruce (Picea glauca) were tested against eight ten-base oligonucleotide primers. Most of the tested primer/parent tree combinations yielded polymorphic PCR products ("RAPD" markers). Selected primers were then used in PCR reactions with template DNA isolated from offspring in Douglas-fir and black spruce diallel crosses among the same parental lines.
653 $aConifers-Genetic-Markers
653 $aLinkage mapping
653 $aRandom amplified DNA markers
700 1 $aTULSIERAM, L. K.
700 1 $aGLAUBITZ, J. C.
700 1 $aLUK, V. W. K.
700 1 $aKAUFFELDT, C.
700 1 $aRUTLEDGE, R.
773 $tTheor Appl Genet$gv.83, p.194-200, 1991
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
