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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
16/10/2002 |
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Data da última atualização: |
16/10/2002 |
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Autoria: |
TSAI, S. J.; ANDERSON, L. E.; JUENGEL, J.; NISWENDER, G. D.; WILTBANK, M. C. |
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Título: |
Regulation of prostaglandin F2alpha and E receptor mRNA by prostaglandin F2alpha in ovine corpora lutea. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Journal of Reproduction and Fertility, v. 114, n. 1, p. 69-75, 1998. |
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Idioma: |
Inglês |
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Conteúdo: |
Prostaglandins regulate many physiological functions, including reproduction, by binding to specific plasma membrane receptors. In this study we evaluated the regulation of PGF 2alpha (FP) and PGE (EP 3 subtype) receptors in ovine corpora lutea. In the first study, tissue distribution of FP and EP 3 receptors was evaluated in 13 ovine tissues. FP receptor rnRNA was present in 100-fold higher concentration in corpora lutea than in other tissues. Similarly, [3H]PGF2alpha binding was much greater in luteal plasma membranes than in membranes from other tissues. In contrast, EP 3 receptor rnRNA was more uniformly distributed, with high concentrations in adrenal medulla, inner myometrium, kidney medulla and heart. The distribution of [3H]PGE1 binding was generally similar to EP 3 rnRNA, with the exception that ovarian stroma, endometrium and outer myometrium had high [3H]PGE1 binding but low concentrations of EP 3 receptor mRNA. The second study evaluated the action of PGF2" on luteal rnRNA encoding FP and EP 3 receptors. Ewes had PGF 2alpha or saline infused into the ovarian artery and corpora lutea were removed at O, 1,4, 12 and 24 h. FP receptor mRNA decreased by 50% at 12 and 24 h after infusion with PGF 2alpha whereas EP 3 rnRNA was unchanged. Treatment of large luteal cells with PGF2alpha' phorbol didecanoate (protein kinase C activator), or ionomycin (calcium ionophore) decreased FP receptor rnRNA after 24 h (P < 0.05). Glyceraldehyde 3-phosphate dehydrogenase rnRNA was not changed by any treatment. These results show that EP3 receptors are expressed in many tissues and expression is not regulated by PGF 2alpha. In contrast, FP receptors are primarily expressed in corpora lutea and expression is inhibited by PGF 2alpha. MenosProstaglandins regulate many physiological functions, including reproduction, by binding to specific plasma membrane receptors. In this study we evaluated the regulation of PGF 2alpha (FP) and PGE (EP 3 subtype) receptors in ovine corpora lutea. In the first study, tissue distribution of FP and EP 3 receptors was evaluated in 13 ovine tissues. FP receptor rnRNA was present in 100-fold higher concentration in corpora lutea than in other tissues. Similarly, [3H]PGF2alpha binding was much greater in luteal plasma membranes than in membranes from other tissues. In contrast, EP 3 receptor rnRNA was more uniformly distributed, with high concentrations in adrenal medulla, inner myometrium, kidney medulla and heart. The distribution of [3H]PGE1 binding was generally similar to EP 3 rnRNA, with the exception that ovarian stroma, endometrium and outer myometrium had high [3H]PGE1 binding but low concentrations of EP 3 receptor mRNA. The second study evaluated the action of PGF2" on luteal rnRNA encoding FP and EP 3 receptors. Ewes had PGF 2alpha or saline infused into the ovarian artery and corpora lutea were removed at O, 1,4, 12 and 24 h. FP receptor mRNA decreased by 50% at 12 and 24 h after infusion with PGF 2alpha whereas EP 3 rnRNA was unchanged. Treatment of large luteal cells with PGF2alpha' phorbol didecanoate (protein kinase C activator), or ionomycin (calcium ionophore) decreased FP receptor rnRNA after 24 h (P < 0.05). Glyceraldehyde 3-phosphate dehydrogenase rnRNA was n... Mostrar Tudo |
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Thesagro: |
Corpo Lúteo; Ovino; Prostaglandina. |
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Categoria do assunto: |
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Marc: |
LEADER 02356naa a2200205 a 4500
001 1528632
005 2002-10-16
008 1998 bl uuuu u00u1 u #d
100 1 $aTSAI, S. J.
245 $aRegulation of prostaglandin F2alpha and E receptor mRNA by prostaglandin F2alpha in ovine corpora lutea.
260 $c1998
520 $aProstaglandins regulate many physiological functions, including reproduction, by binding to specific plasma membrane receptors. In this study we evaluated the regulation of PGF 2alpha (FP) and PGE (EP 3 subtype) receptors in ovine corpora lutea. In the first study, tissue distribution of FP and EP 3 receptors was evaluated in 13 ovine tissues. FP receptor rnRNA was present in 100-fold higher concentration in corpora lutea than in other tissues. Similarly, [3H]PGF2alpha binding was much greater in luteal plasma membranes than in membranes from other tissues. In contrast, EP 3 receptor rnRNA was more uniformly distributed, with high concentrations in adrenal medulla, inner myometrium, kidney medulla and heart. The distribution of [3H]PGE1 binding was generally similar to EP 3 rnRNA, with the exception that ovarian stroma, endometrium and outer myometrium had high [3H]PGE1 binding but low concentrations of EP 3 receptor mRNA. The second study evaluated the action of PGF2" on luteal rnRNA encoding FP and EP 3 receptors. Ewes had PGF 2alpha or saline infused into the ovarian artery and corpora lutea were removed at O, 1,4, 12 and 24 h. FP receptor mRNA decreased by 50% at 12 and 24 h after infusion with PGF 2alpha whereas EP 3 rnRNA was unchanged. Treatment of large luteal cells with PGF2alpha' phorbol didecanoate (protein kinase C activator), or ionomycin (calcium ionophore) decreased FP receptor rnRNA after 24 h (P < 0.05). Glyceraldehyde 3-phosphate dehydrogenase rnRNA was not changed by any treatment. These results show that EP3 receptors are expressed in many tissues and expression is not regulated by PGF 2alpha. In contrast, FP receptors are primarily expressed in corpora lutea and expression is inhibited by PGF 2alpha.
650 $aCorpo Lúteo
650 $aOvino
650 $aProstaglandina
700 1 $aANDERSON, L. E.
700 1 $aJUENGEL, J.
700 1 $aNISWENDER, G. D.
700 1 $aWILTBANK, M. C.
773 $tJournal of Reproduction and Fertility$gv. 114, n. 1, p. 69-75, 1998.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
22/05/2007 |
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Data da última atualização: |
02/04/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TEIXEIRA, L. O. A.; PAIVA, J. R. de; GONÇALVES, P. de S. |
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Afiliação: |
EMBRAPA-CNPSD. |
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Título: |
Conservação e avaliação de recursos genéticos de seringueira. |
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Ano de publicação: |
1984 |
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Fonte/Imprenta: |
In: SEMINÁRIO NACIONAL DA SERINGUEIRA, 4., 1984, Salvador. Resumo dos trabalhos. [S.l.]: SUDHEVEA/EMBRAPA/EMBRATER/Banco Econômico, [1984]. p. 50-51. |
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Idioma: |
Português |
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Conteúdo: |
A grande variabilidade genética da seringueira é uma das principais riquezas de que dispõem os melhoristas para ampliar as potencialidades de cultivo dessa espécie vegetal. |
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Thesagro: |
Recurso Genético; Seringueira. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/121755/1/p.-50-51.pdf
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Marc: |
LEADER 00738nam a2200157 a 4500
001 1680484
005 2015-04-02
008 1984 bl uuuu u00u1 u #d
100 1 $aTEIXEIRA, L. O. A.
245 $aConservação e avaliação de recursos genéticos de seringueira.
260 $aIn: SEMINÁRIO NACIONAL DA SERINGUEIRA, 4., 1984, Salvador. Resumo dos trabalhos. [S.l.]: SUDHEVEA/EMBRAPA/EMBRATER/Banco Econômico, [1984]. p. 50-51.$c1984
520 $aA grande variabilidade genética da seringueira é uma das principais riquezas de que dispõem os melhoristas para ampliar as potencialidades de cultivo dessa espécie vegetal.
650 $aRecurso Genético
650 $aSeringueira
700 1 $aPAIVA, J. R. de
700 1 $aGONÇALVES, P. de S.
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Embrapa Amazônia Ocidental (CPAA) |
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