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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
26/12/2019 |
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Data da última atualização: |
13/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BLECHA, I. M. Z.; SOUSA, I. I.; FERREIRA, A. B. R.; FEIJO, G. L. D.; TORRES JUNIOR, R. A. de A.; SIQUEIRA, F. |
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Afiliação: |
Isabella Maiumi Zaidan Blecha, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal; Isadora Inácio Sousa, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal; ANNA BEATRIZ ROBOTTOM FERREIRA, CTAA; GELSON LUIS DIAS FEIJO, CNPGC; ROBERTO AUGUSTO DE A TORRES JUNIOR, CNPGC; FABIANE SIQUEIRA, CNPGC. |
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Título: |
Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 48, e20180218, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels. MenosThe objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform i... Mostrar Tudo |
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Thesaurus Nal: |
Animal breeding; Marker-assisted selection; Meat tenderness. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207815/1/Alternative-methodologies-for-genotyping.pdf
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Marc: |
LEADER 02589naa a2200217 a 4500
001 2117678
005 2020-01-13
008 2019 bl uuuu u00u1 u #d
100 1 $aBLECHA, I. M. Z.
245 $aAlternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle.$h[electronic resource]
260 $c2019
520 $aThe objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels.
650 $aAnimal breeding
650 $aMarker-assisted selection
650 $aMeat tenderness
700 1 $aSOUSA, I. I.
700 1 $aFERREIRA, A. B. R.
700 1 $aFEIJO, G. L. D.
700 1 $aTORRES JUNIOR, R. A. de A.
700 1 $aSIQUEIRA, F.
773 $tRevista Brasileira de Zootecnia$gv. 48, e20180218, 2019.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
21/02/2013 |
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Data da última atualização: |
30/08/2013 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
OLIVEIRA, L. C. de; ISHIDA, A. K. N.; BONFIM, K.; BOARI, A. de J. |
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Afiliação: |
LUANA CARDOSO DE OLIVEIRA, UFPA; ALESSANDRA KEIKO NAKASONE ISHIDA, CPATU; KENNY BONFIM DE ARRUDA CARVALHO, CPATU; ALESSANDRA DE JESUS BOARI, CPATU. |
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Título: |
Especificidade dos primers PAS-R/PAS3-D e PAS-R/PAS-D na detecção de isolados de Xanthomonas axonopodis pv. passiflorae do estado do Pará. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
A mancha bacteriana (Xanthomonas axonopodis pv. passiflorae) destaca-se entre as doenças da cultura do maracujazeiro. Ocorre sob condições de umidade e temperaturas altas e se encontra disseminada no Estado do Pará. O objetivo deste trabalho foi avaliar a especificidade dos pares de primers Pas-R/Pas3-D e Pas-R/Pas-D na detecção de 29 isolados de X. axonopodis pv. passiflorae provenientes de diferentes regiões produtoras de maracujá do Estado. Verificou-se que os primers Pas-R e Pas3-D amplificaram 2 fragmentos de aproximadamente 750pb e 250pb nos isolados de X. axonopodis pv. passiflorae. Enquanto que os primers Pas-R e Pas-D amplificaram 1 fragmento de aproximadamente 1000pb. |
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Palavras-Chave: |
Mancha bacteriana do maracujazeiro; Primers; Variabilidade genética. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/76898/1/121.pdf
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Marc: |
LEADER 01420nam a2200193 a 4500
001 1950381
005 2013-08-30
008 2012 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, L. C. de
245 $aEspecificidade dos primers PAS-R/PAS3-D e PAS-R/PAS-D na detecção de isolados de Xanthomonas axonopodis pv. passiflorae do estado do Pará.
260 $aIn: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos$c2012
300 $c1 CD-ROM.
520 $aA mancha bacteriana (Xanthomonas axonopodis pv. passiflorae) destaca-se entre as doenças da cultura do maracujazeiro. Ocorre sob condições de umidade e temperaturas altas e se encontra disseminada no Estado do Pará. O objetivo deste trabalho foi avaliar a especificidade dos pares de primers Pas-R/Pas3-D e Pas-R/Pas-D na detecção de 29 isolados de X. axonopodis pv. passiflorae provenientes de diferentes regiões produtoras de maracujá do Estado. Verificou-se que os primers Pas-R e Pas3-D amplificaram 2 fragmentos de aproximadamente 750pb e 250pb nos isolados de X. axonopodis pv. passiflorae. Enquanto que os primers Pas-R e Pas-D amplificaram 1 fragmento de aproximadamente 1000pb.
653 $aMancha bacteriana do maracujazeiro
653 $aPrimers
653 $aVariabilidade genética
700 1 $aISHIDA, A. K. N.
700 1 $aBONFIM, K.
700 1 $aBOARI, A. de J.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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