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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
04/07/2013 |
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Data da última atualização: |
25/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TORRES, A. R.; ARAÚJO, W. L.; CURSINO, L.; ROSSETTO, P. de B.; MONDIN, M.; HUNGRIA, M.; AZEVEDO, J. L. |
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Afiliação: |
ADALGISA RIBEIRO TORRES, ESALQ; WELINGTON LUIZ ARAÚJO, USP; LUCIANA CURSINO, ESALQ; PRISCILLA DE BARROS ROSSETTO, UFRJ; MATEUS MONDIN, ESALQ; MARIANGELA HUNGRIA DA CUNHA, CNPSO; JOÃO LÚCIO AZEVEDO, ESALQ. |
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Título: |
Colonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Archives of Microbiology, Berlin, v. 195, n. 7, p. 483-489, Jul. 2013. |
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Idioma: |
Português |
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Conteúdo: |
This study reports the introduction of gfp marker in two endophytic bacterial strains (Pantoea agglomerans C33.1, isolated from cocoa, and Enterobacter cloacae PR2/7, isolated from citrus) to monitor the colonization in Madagascar perinwinkle (Catharanthus roseus). Stability of the plasmid encoding gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under non-selective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated perinwinkle plants that grew for 10 and 20 days. Gfp-expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the P. agglomerans C33:1gfp in plants 20 days after inoculation, even in the absence of selective pressure, suggests that is good colonizer. These results indicated that both gfptagged strains, especially P. agglomerans C33.1, may be useful tools to deliver enzymes or other proteins in plant. |
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Palavras-Chave: |
Bactéria endofítica. |
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Categoria do assunto: |
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Marc: |
LEADER 01666naa a2200205 a 4500
001 1961415
005 2017-07-25
008 2013 bl uuuu u00u1 u #d
100 1 $aTORRES, A. R.
245 $aColonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker.$h[electronic resource]
260 $c2013
520 $aThis study reports the introduction of gfp marker in two endophytic bacterial strains (Pantoea agglomerans C33.1, isolated from cocoa, and Enterobacter cloacae PR2/7, isolated from citrus) to monitor the colonization in Madagascar perinwinkle (Catharanthus roseus). Stability of the plasmid encoding gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under non-selective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated perinwinkle plants that grew for 10 and 20 days. Gfp-expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the P. agglomerans C33:1gfp in plants 20 days after inoculation, even in the absence of selective pressure, suggests that is good colonizer. These results indicated that both gfptagged strains, especially P. agglomerans C33.1, may be useful tools to deliver enzymes or other proteins in plant.
653 $aBactéria endofítica
700 1 $aARAÚJO, W. L.
700 1 $aCURSINO, L.
700 1 $aROSSETTO, P. de B.
700 1 $aMONDIN, M.
700 1 $aHUNGRIA, M.
700 1 $aAZEVEDO, J. L.
773 $tArchives of Microbiology, Berlin$gv. 195, n. 7, p. 483-489, Jul. 2013.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
30/03/1998 |
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Data da última atualização: |
28/12/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MACEDO, J. L. V. de; MORAES, C. R. de A.; MOTA, A. M. |
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Afiliação: |
JEFERSON LUIS VASCONCELOS DE MACEDO, CPAA. |
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Título: |
Production of cupuaçu (Theobroma grandiflorum) in different polyculture systems. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
In: SHIFT-WORKSHOP, 3., 1998, Manaus. Program, abstracts of presentations and posters... Hamburg: GKSS, 1998. |
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Páginas: |
p. C4. |
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Idioma: |
Inglês |
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Conteúdo: |
A summary about evaluation the cupuacu plants in three agroforestry systems. |
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Palavras-Chave: |
Agrofloresta; Amazonas; Brasil. |
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Thesagro: |
Cultivo Multiplo; Cupuaçu; Theobroma Grandiflorum. |
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Thesaurus NAL: |
agroforestry; multiple cropping. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/124249/1/C4.pdf
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Marc: |
LEADER 00798nam a2200241 a 4500
001 1667697
005 2018-12-28
008 1998 bl uuuu u00u1 u #d
100 1 $aMACEDO, J. L. V. de
245 $aProduction of cupuaçu (Theobroma grandiflorum) in different polyculture systems.
260 $aIn: SHIFT-WORKSHOP, 3., 1998, Manaus. Program, abstracts of presentations and posters... Hamburg: GKSS$c1998
300 $ap. C4.
520 $aA summary about evaluation the cupuacu plants in three agroforestry systems.
650 $aagroforestry
650 $amultiple cropping
650 $aCultivo Multiplo
650 $aCupuaçu
650 $aTheobroma Grandiflorum
653 $aAgrofloresta
653 $aAmazonas
653 $aBrasil
700 1 $aMORAES, C. R. de A.
700 1 $aMOTA, A. M.
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