|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
19/09/2018 |
|
Data da última atualização: |
19/09/2018 |
|
Autoria: |
THOMPSON, J. G.; SIMPSON, A. C.; PUGH, P. A.; WRIGHT JUNIOR, R. W.; TERVIT, H. R. |
|
Título: |
Glucose utilization by sheep embryos derived in vivo and in vitro. |
|
Ano de publicação: |
1991 |
|
Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 3, n. 5, p. 571-576, 1991. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Embryos were collected from superovulated donors at various intervals from onset of oestrus, ranging from Day 1.5 to Day 6. In addition, blastocysts obtained from the culture of 1-cell embryos collected in vivo or of oocytes matured and fertilized in vitro were used to assess the effects of in vitro manipulation and culture on glucose utilization. Glycolytic activity was determined by the conversion of [5-3H]glucose to 3H2O, and oxidation of glucose was determined by the conversion of [U-14C]glucose to 14CO2. Glucose utilization increases significantly from the 8-cell stage and during compaction and blastulation. Glucose oxidation was at a relatively low level (5-12% of total utilization) compared with glycolysis. No difference was observed between the glycolytic activity of blastocysts derived from in vivo or in vitro sources. However, glucose oxidation was lower (P less than 0.05) in blastocysts derived from the culture of 1-cell embryos or from oocytes matured and fertilized in vitro. Exogenous tricarboxylic acid cycle substrates (i.e. pyruvate and lactate supplied in the medium) affected the level of glucose oxidation. |
|
Palavras-Chave: |
Cleavage Stage Ovum; Dinitrophenols; Drug effects; Pyruvates. |
|
Thesaurus Nal: |
2,4-dinitrophenol; Blastocyst; Drug interactions; Fetus; Glucose; Glycolysis; In vitro fertilization; Lactates; Lactic acid; Metabolism; Morula; Pharmacology; Pyruvic acid; Sheep. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02150naa a2200385 a 4500
001 2096010
005 2018-09-19
008 1991 bl uuuu u00u1 u #d
100 1 $aTHOMPSON, J. G.
245 $aGlucose utilization by sheep embryos derived in vivo and in vitro.
260 $c1991
520 $aAbstract: Embryos were collected from superovulated donors at various intervals from onset of oestrus, ranging from Day 1.5 to Day 6. In addition, blastocysts obtained from the culture of 1-cell embryos collected in vivo or of oocytes matured and fertilized in vitro were used to assess the effects of in vitro manipulation and culture on glucose utilization. Glycolytic activity was determined by the conversion of [5-3H]glucose to 3H2O, and oxidation of glucose was determined by the conversion of [U-14C]glucose to 14CO2. Glucose utilization increases significantly from the 8-cell stage and during compaction and blastulation. Glucose oxidation was at a relatively low level (5-12% of total utilization) compared with glycolysis. No difference was observed between the glycolytic activity of blastocysts derived from in vivo or in vitro sources. However, glucose oxidation was lower (P less than 0.05) in blastocysts derived from the culture of 1-cell embryos or from oocytes matured and fertilized in vitro. Exogenous tricarboxylic acid cycle substrates (i.e. pyruvate and lactate supplied in the medium) affected the level of glucose oxidation.
650 $a2,4-dinitrophenol
650 $aBlastocyst
650 $aDrug interactions
650 $aFetus
650 $aGlucose
650 $aGlycolysis
650 $aIn vitro fertilization
650 $aLactates
650 $aLactic acid
650 $aMetabolism
650 $aMorula
650 $aPharmacology
650 $aPyruvic acid
650 $aSheep
653 $aCleavage Stage Ovum
653 $aDinitrophenols
653 $aDrug effects
653 $aPyruvates
700 1 $aSIMPSON, A. C.
700 1 $aPUGH, P. A.
700 1 $aWRIGHT JUNIOR, R. W.
700 1 $aTERVIT, H. R.
773 $tReproduction, Fertility and Development$gv. 3, n. 5, p. 571-576, 1991.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
