Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
09/05/1996 |
Data da última atualização: |
31/05/2023 |
Autoria: |
GIBB, K. S; PERSLEY, SCHNEIDER, B; THOMAS, J. E. |
Afiliação: |
Faculty of Science, Northern Territory University, Darwin, Norther Territory, 0909, Australia, D.M. |
Título: |
Phytoplasmas Associated With Papaya Diseases in australia |
Ano de publicação: |
1996 |
Fonte/Imprenta: |
Plant disease, n. 2, v. 80, 174-178, 1996. |
Idioma: |
Inglês |
Conteúdo: |
leaf, petiole, and crown samples were taken from papaya with dieback or mosaic diseases, which have an unknown etiology, and from yellow crinkle disease, which has been associated with a phytoplasma. Using the polymerase chian reaction (PCR) and phytoplasma-specific primers, an amplification product of the expected size (1,076 bp) was observed in samples from papaya with yellow crinkle, mosaic, and dieback, and in none of the samples from asymptomatic plants. A second set of phytoplasma-specific primers was used with samples from each of three diseases to produce a PCR product of 1,800 bp for restriction fragment length polymorphism (RFLP) analysis of phytoplasma relatedness. The phytoplasma associated with yellow crinkle and mosaic were identical by RFLP analysis using three restriction enzymes, but the phytoplasma from dieback disease differented from the other two. The phytoplasma associated with dieback is related to the Stolbur phytoplasmas that form part of the aster yellows phytoplasma strain cluster. The phytoplasma associated with yellow crinkle and mosaic, along with sweet potato little leaf, appears to be related to a phytoplasma, crotalaria witches-broom from Thailand, which in turn is related to the western X-phytoplasma. |
Palavras-Chave: |
Fitoplasma; Mosaic. |
Thesagro: |
Carica Papaya; Mamão; Mosaico; Vírus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01792naa a2200217 a 4500 001 1634308 005 2023-05-31 008 1996 bl uuuu u00u1 u #d 100 1 $aGIBB, K. S 245 $aPhytoplasmas Associated With Papaya Diseases in australia$h[electronic resource] 260 $c1996 520 $aleaf, petiole, and crown samples were taken from papaya with dieback or mosaic diseases, which have an unknown etiology, and from yellow crinkle disease, which has been associated with a phytoplasma. Using the polymerase chian reaction (PCR) and phytoplasma-specific primers, an amplification product of the expected size (1,076 bp) was observed in samples from papaya with yellow crinkle, mosaic, and dieback, and in none of the samples from asymptomatic plants. A second set of phytoplasma-specific primers was used with samples from each of three diseases to produce a PCR product of 1,800 bp for restriction fragment length polymorphism (RFLP) analysis of phytoplasma relatedness. The phytoplasma associated with yellow crinkle and mosaic were identical by RFLP analysis using three restriction enzymes, but the phytoplasma from dieback disease differented from the other two. The phytoplasma associated with dieback is related to the Stolbur phytoplasmas that form part of the aster yellows phytoplasma strain cluster. The phytoplasma associated with yellow crinkle and mosaic, along with sweet potato little leaf, appears to be related to a phytoplasma, crotalaria witches-broom from Thailand, which in turn is related to the western X-phytoplasma. 650 $aCarica Papaya 650 $aMamão 650 $aMosaico 650 $aVírus 653 $aFitoplasma 653 $aMosaic 700 1 $aPERSLEY, SCHNEIDER, B 700 1 $aTHOMAS, J. E. 773 $tPlant disease$gn. 2
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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