Data/hora: 19/06/2024 05:17:04

Biblioteca(s): | Embrapa Agroindústria Tropical.
Data corrente: | 24/06/1993
Data da última atualização: | 24/06/1993
Autoria: | CASTELFRANCO, P. A.; THAYER, S. S.; WILKINSON, J. Q.; BONNER, B. A.
Título: | Labeling of porphobilinogen deaminase by radioactive 5 - Aminolevulinic Acid in isolated developing pea chloroplasts.
Ano de publicação: | 1988
Fonte/Imprenta: | The SASEB Journal, v.2, p.3003-3009, Nov. 1988.Archives of Biochemistry and Biophysics, v.266, n.1, p.219-226, 1988.
Idioma: | Inglês
Conteúdo: | A protein had been previously described, which was labeled by radioactive 5-aminolevulinic acid in isolated developing chloroplasts. In the present study we have shown that this protein (Mr=~43,000) probably exists as a monomer in the chloroplast stroma. The labeling is blocked if known inhibitors of 5 - aminolevulinic acid dehydratase are added to the incubation mixture, and is markedly decreased in intensity if nonradioactive 5 - aminolevulinate or porphobilinogen are added to the incubation mixture; other intermediates in the porphyrin biosynthetic pathway, uroporphyrinigen II, uroporphyrin III, and protoporphyrin IX, do not decrease the labeling of the 43-kDa protein appreciably. Nondenaturing gels of the proteins isolated from the incubation with coincided with the radioactive bands visualized by autoradiography. It is concluded that the soluble chloroplast protein that is labeled in organello by radioactive 5 - aminolevulinic acid is porphobilinogen deaminase.
Palavras-Chave: | Ervilha - cloroplasto - desenvolvimento; Ervilha - eletroforese; Ervilha - incubacao.
Categoria do assunto: | --
Registro original: | Embrapa Agroindústria Tropical (CNPAT)
Exemplares: | CNPAT - (550-1) - EMB - SP - -- - SP 00668 -  - 90.00668 - DIS