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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
23/08/2012 |
Data da última atualização: |
22/02/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; MELO, D. S. de; NICIURA, S. C. M.; GARCIA, J. M. |
Afiliação: |
NAIARA ZOCCAL SARAIVA, Universidade Estadual Paulista; CLARA SLADE OLIVEIRA, UNESP; TATIANE ALMEIDA DRUMMOND TETZNER, UNESP; MARINA RAGAGNIN DE LIMA, UNESP/JABOTICABAL; DANILAS SALINET DE MELO, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
Título: |
Chemically assisted enucleation results in higher G6PD expression in early bovine female embryos obtained by somatic cell nuclear transfer. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Cellular Reprogramming, v. 14, n. 5, p. 1-11, 2012. |
DOI: |
10.1089/cell.2011.0077 |
Idioma: |
Inglês |
Conteúdo: |
Despite extensive efforts, low efficiency is still an issue in bovine somatic cell nuclear transfer (SCNT). The hypothesis of our study was that the use of cytoplasts produced by chemically assisted enucleation (EN) would improve nuclear reprogramming in nuclear transfer (NT)?derived embryos because it results in lower damage and higher cytoplasm content than conventional EN. For that purpose, we investigated the expression of two X-linked genes: X inactive-specific transcript (XIST) and glucose 6-phosphate dehydrogenase (G6PD). In the first experiment, gene expression was assessed in day-7 female blastocysts from embryonic cell NT (ECNT) groups [conventional, ECNT conv; chemically assisted, ECNT deme (demecolcine)]. Where as in the ECNT conv group, only one embryo (25%; n = 4) expressed XIST transcripts, most embryos showed XIST expression (75%; n = 4) in the ECNT deme group. However, no significant differences in transcript abundance of XIST and G6PD were found when comparing the embryos from all groups. In a second experiment using somatic cells as nuclear donors, we evaluated gene expression profiles in female SCNT-derived embryos. No significant differences in relative abundance (RA) of XIST transcripts were observed among the groups. Nonetheless, higher ( p < 0.05) levels of G6PD were observed in SCNT deme and in vitro?derived groups in comparison to SCNT conv. To know whether higher G6PD expression in embryos derived from SCNT chemically assisted EN indicates higher metabolism in embryos considered of superior quality or if the presence of higher reactive oxygen species (ROS) levels generated by the increased oxygen consumption triggers G6PD activation, the expression of genes related to stress response should be investigated in embryos produced by that technique. MenosDespite extensive efforts, low efficiency is still an issue in bovine somatic cell nuclear transfer (SCNT). The hypothesis of our study was that the use of cytoplasts produced by chemically assisted enucleation (EN) would improve nuclear reprogramming in nuclear transfer (NT)?derived embryos because it results in lower damage and higher cytoplasm content than conventional EN. For that purpose, we investigated the expression of two X-linked genes: X inactive-specific transcript (XIST) and glucose 6-phosphate dehydrogenase (G6PD). In the first experiment, gene expression was assessed in day-7 female blastocysts from embryonic cell NT (ECNT) groups [conventional, ECNT conv; chemically assisted, ECNT deme (demecolcine)]. Where as in the ECNT conv group, only one embryo (25%; n = 4) expressed XIST transcripts, most embryos showed XIST expression (75%; n = 4) in the ECNT deme group. However, no significant differences in transcript abundance of XIST and G6PD were found when comparing the embryos from all groups. In a second experiment using somatic cells as nuclear donors, we evaluated gene expression profiles in female SCNT-derived embryos. No significant differences in relative abundance (RA) of XIST transcripts were observed among the groups. Nonetheless, higher ( p < 0.05) levels of G6PD were observed in SCNT deme and in vitro?derived groups in comparison to SCNT conv. To know whether higher G6PD expression in embryos derived from SCNT chemically assisted EN indicates higher m... Mostrar Tudo |
Palavras-Chave: |
Higher G6PD; Nuclear transfer; Somatic cell. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02567naa a2200241 a 4500 001 1932049 005 2023-02-22 008 2012 bl uuuu u00u1 u #d 024 7 $a10.1089/cell.2011.0077$2DOI 100 1 $aSARAIVA, N. Z. 245 $aChemically assisted enucleation results in higher G6PD expression in early bovine female embryos obtained by somatic cell nuclear transfer.$h[electronic resource] 260 $c2012 520 $aDespite extensive efforts, low efficiency is still an issue in bovine somatic cell nuclear transfer (SCNT). The hypothesis of our study was that the use of cytoplasts produced by chemically assisted enucleation (EN) would improve nuclear reprogramming in nuclear transfer (NT)?derived embryos because it results in lower damage and higher cytoplasm content than conventional EN. For that purpose, we investigated the expression of two X-linked genes: X inactive-specific transcript (XIST) and glucose 6-phosphate dehydrogenase (G6PD). In the first experiment, gene expression was assessed in day-7 female blastocysts from embryonic cell NT (ECNT) groups [conventional, ECNT conv; chemically assisted, ECNT deme (demecolcine)]. Where as in the ECNT conv group, only one embryo (25%; n = 4) expressed XIST transcripts, most embryos showed XIST expression (75%; n = 4) in the ECNT deme group. However, no significant differences in transcript abundance of XIST and G6PD were found when comparing the embryos from all groups. In a second experiment using somatic cells as nuclear donors, we evaluated gene expression profiles in female SCNT-derived embryos. No significant differences in relative abundance (RA) of XIST transcripts were observed among the groups. Nonetheless, higher ( p < 0.05) levels of G6PD were observed in SCNT deme and in vitro?derived groups in comparison to SCNT conv. To know whether higher G6PD expression in embryos derived from SCNT chemically assisted EN indicates higher metabolism in embryos considered of superior quality or if the presence of higher reactive oxygen species (ROS) levels generated by the increased oxygen consumption triggers G6PD activation, the expression of genes related to stress response should be investigated in embryos produced by that technique. 653 $aHigher G6PD 653 $aNuclear transfer 653 $aSomatic cell 700 1 $aOLIVEIRA, C. S. 700 1 $aTETZNER, T. A. D. 700 1 $aLIMA, M. R. de 700 1 $aMELO, D. S. de 700 1 $aNICIURA, S. C. M. 700 1 $aGARCIA, J. M. 773 $tCellular Reprogramming$gv. 14, n. 5, p. 1-11, 2012.
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Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
12/01/2018 |
Data da última atualização: |
23/09/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ORIENTE, V. N.; MONTEIRO, J. P.; TEIXEIRA, M. |
Afiliação: |
Valcledes Nascimento Oriente; JOMAR PATRICIO MONTEIRO, CNPC; MARCEL TEIXEIRA, CNPC. |
Título: |
Acurácia do método FAMACHA no controle seletivo das helmintoses gastrintestinas de caprinos e ovinos. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 6., 2017, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2017. p. 58-59. |
Idioma: |
Português |
Conteúdo: |
O método FAMACHA© é uma ferramenta sujeita a erros de interpretação principalmente em caprinos. De acordo com a acurácia encontrada a técnica continua sendo recomendada no controle seletivo das helmintoses gastrintestinais de pequenos ruminantes. |
Palavras-Chave: |
Controle; Helminthoses; Helmintose. |
Thesagro: |
Anemia Infecciosa; Caprino; Haemonchus Contortus; Helminto gastrintestinal; Nematóide; Ovino; Verminose. |
Thesaurus NAL: |
Goats; Sheep. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171931/1/CNPC-2017-Acuracia.pdf
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Marc: |
LEADER 01133nam a2200277 a 4500 001 2085239 005 2019-09-23 008 2017 bl uuuu u00u1 u #d 100 1 $aORIENTE, V. N. 245 $aAcurácia do método FAMACHA no controle seletivo das helmintoses gastrintestinas de caprinos e ovinos.$h[electronic resource] 260 $aIn: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 6., 2017, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2017. p. 58-59.$c2017 520 $aO método FAMACHA© é uma ferramenta sujeita a erros de interpretação principalmente em caprinos. De acordo com a acurácia encontrada a técnica continua sendo recomendada no controle seletivo das helmintoses gastrintestinais de pequenos ruminantes. 650 $aGoats 650 $aSheep 650 $aAnemia Infecciosa 650 $aCaprino 650 $aHaemonchus Contortus 650 $aHelminto gastrintestinal 650 $aNematóide 650 $aOvino 650 $aVerminose 653 $aControle 653 $aHelminthoses 653 $aHelmintose 700 1 $aMONTEIRO, J. P. 700 1 $aTEIXEIRA, M.
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