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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/09/1995 |
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Data da última atualização: |
06/09/1995 |
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Autoria: |
SANTOS, C. D.; TERRA, W. R. |
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Afiliação: |
Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo. c.p. 20780, Sao paulo, Brasil. |
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Título: |
Intracelular Distribution of Digestive Enzymes in the Midgut of the Cassava Hornworm, Erinnyis Ello. |
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Ano de publicação: |
1984 |
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Fonte/Imprenta: |
Insect. Biochem, v.14, n.5, p.587-594, 1984. |
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Idioma: |
Inglês |
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Conteúdo: |
Differential centrifugation of homogenates of midgut cells prepared in isotonic solutions has been carried out and hydrolase and enzyme marker activities have been determined in the isolated fractions a-and B-Glucosidase and trehalase seem to occur loosely associated with large structures, from which they are set free by homogenizing in water. They are also found in the cytosolic fraction. Aminopeptidase in microvili purified from midgut cells is 4 and for amylase 1.5. Amilase and trypsin are only partly released from a membrance fraction after several washings in different media, including ultracentrifugation in a discontinuous glycerol gradient. About 50% of the amylase and trypsin are solubilized from the membranes by treatment with Triton X-100. The results support the proposal that intermediante and final disgestion in Erinnyis ello occur under the action of glicocalyx-associated hydrolases (a-and B-glucosidase) and of plasma membrane-bound enzymes 9aminopeptidase, and perhaps also amylase and trypsin. |
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Palavras-Chave: |
Final digestion; glycocalyx-associated enzymes; Subcellular distribution of enzymes. |
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Thesagro: |
Erinnyis Ello. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01579naa a2200181 a 4500
001 1647000
005 1995-09-06
008 1984 bl --- 0-- u #d
100 1 $aSANTOS, C. D.
245 $aIntracelular Distribution of Digestive Enzymes in the Midgut of the Cassava Hornworm, Erinnyis Ello.
260 $c1984
520 $aDifferential centrifugation of homogenates of midgut cells prepared in isotonic solutions has been carried out and hydrolase and enzyme marker activities have been determined in the isolated fractions a-and B-Glucosidase and trehalase seem to occur loosely associated with large structures, from which they are set free by homogenizing in water. They are also found in the cytosolic fraction. Aminopeptidase in microvili purified from midgut cells is 4 and for amylase 1.5. Amilase and trypsin are only partly released from a membrance fraction after several washings in different media, including ultracentrifugation in a discontinuous glycerol gradient. About 50% of the amylase and trypsin are solubilized from the membranes by treatment with Triton X-100. The results support the proposal that intermediante and final disgestion in Erinnyis ello occur under the action of glicocalyx-associated hydrolases (a-and B-glucosidase) and of plasma membrane-bound enzymes 9aminopeptidase, and perhaps also amylase and trypsin.
650 $aErinnyis Ello
653 $aFinal digestion
653 $aglycocalyx-associated enzymes
653 $aSubcellular distribution of enzymes
700 1 $aTERRA, W. R.
773 $tInsect. Biochem$gv.14, n.5, p.587-594, 1984.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 5 | |
| 1. |  | SILVA, C. P.; TERRA, W. R.; XAVIER FILHO, J.; SA, M. F. G. de; BIFANO, T. D. Induction of [Alfa] Amylases in larvae of the bruchid beetle zabrotes Subfasciatus in response to different hosts. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 28., 1999, Caxambu, MG. Programa e resumos. Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1999. C 35. Producao cientifica Embrapa Recursos Geneticos e Biotecnologia.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | SILVA, C. P.; TERRA, W. R.; XAVIER FILHO, J.; SA, M. F. G. de; BIFANO, T. D. Induction of x-amylases in larvae of the bruchid beetle zabrotes subfasciatus in response to different hosts. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 28., 1999, Caxambu, MG. Programa e resumos. Caxambu: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1999.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | MACEDO, L. L. P.; SOUZA JUNIOR, J. D. D. de; COELHO, R. R.; FONSECA, F. C. A.; FIRMINO, A. A. P.; SILVA, M. C. M.; FRAGOSO, R. R.; ALBUQUERQUE, E. V. S.; SILVA, M. S.; ENGLER, J. de A.; TERRA, W. R.; GROSSI-DE-SA, M. F. Knocking down chitin synthase 2 by RNAi is lethal to the cotton boll weevil. Biotechnology Research and Innovation, v. 1, p. 72-86, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | FIRMINO, A. A. P.; PINHEIRO, D. H.; MOREIRA-PINTO, C. E.; ANTONINO, J. D.; MACEDO, L. L. P.; MARTINS-DE-SA, D.; ARRAES, F. B. M.; COELHO, R. R.; FONSECA, F. C. de A.; SILVA, M. C. M.; ENGLER, J. de A.; SILVA, M. S.; LOURENÇO-TESSUTTI, I. T.; TERRA, W. R.; GROSSI-DE-SA, M. F. RNAi-mediated suppression of Laccase2 impairs cuticle tanning and molting in the cotton boll weevil (Anthonomus grandis). Frontiers in Physiology, v. 11, article 591569, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | COELHO, R. R.; SOUZA JUNIOR, J. D. A. de; FIRMINO, A. A. P.; MACEDO, L. L. P. de; FONSECA, F. C. A.; TERRA, W. R.; ENGLER, G.; ENGLER, J. de A.; SILVA, M. C. M. da; GROSSI-DE-SA, M. F. Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females. Meta Gene, v. 9, p. 173-180, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 5 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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