Data/hora: 07/06/2024 17:05:14

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 06/09/1995
Data da última atualização: | 06/09/1995
Autoria: | SANTOS, C. D.; TERRA, W. R.
Afiliação: | Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo. c.p. 20780, Sao paulo, Brasil.
Título: | Intracelular Distribution of Digestive Enzymes in the Midgut of the Cassava Hornworm, Erinnyis Ello.
Ano de publicação: | 1984
Fonte/Imprenta: | Insect. Biochem, v.14, n.5, p.587-594, 1984.
Idioma: | Inglês
Conteúdo: | Differential centrifugation of homogenates of midgut cells prepared in isotonic solutions has been carried out and hydrolase and enzyme marker activities have been determined in the isolated fractions a-and B-Glucosidase and trehalase seem to occur loosely associated with large structures, from which they are set free by homogenizing in water. They are also found in the cytosolic fraction. Aminopeptidase in microvili purified from midgut cells is 4 and for amylase 1.5. Amilase and trypsin are only partly released from a membrance fraction after several washings in different media, including ultracentrifugation in a discontinuous glycerol gradient. About 50% of the amylase and trypsin are solubilized from the membranes by treatment with Triton X-100. The results support the proposal that intermediante and final disgestion in Erinnyis ello occur under the action of glicocalyx-associated hydrolases (a-and B-glucosidase) and of plasma membrane-bound enzymes 9aminopeptidase, and perhaps also amylase and trypsin.
Palavras-Chave: | Final digestion; glycocalyx-associated enzymes; Subcellular distribution of enzymes.
Thesagro: | Erinnyis Ello.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (16036-1) - ADC - SP - -- -  -  - S10044 - DIS