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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
07/03/2006 |
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Data da última atualização: |
20/03/2006 |
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Autoria: |
BERED, F.; TERRA, T. de F.; SPELLMEIER, M.; BARBOSA NETO, J. F. |
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Título: |
Genetic variation among and within sweet corn populations detected by RAPD and SSR markers. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 5, n. 4, p. 418-425, Dec. 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
The main objective of the present study was to characterize the genetic structure of three Brazilian sweet corn populations. In addition, we aimed to investigate the molecular genetic diversity within and among the populations and evaluate the ability of RAPD and SSR markers to detect inter and intra-population variation in these populations. PCR were performed using 11 RAPD and six microsatellite primer pairs. Genetic similarity was estimated within and among populations considering the markers independently and jointly. Variability for each locus was measured using the Polymorphism Information Content and the correlation between pairwise distance matrices was obtained. The genetic similarity and analysis of the molecular variance results indicated a higher similarity among than within populations, and RAPD showed lower PIC values than SSR. Knowledge on the genetic diversity distribution in this germplasm is important to determine strategies for future conservation and selection for breeding programs.. |
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Palavras-Chave: |
Diversidade genética; Genetic diversity; Germoplama; Marcadores moleculares; Molecular markers. |
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Thesagro: |
Milho; Zea Mays. |
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Thesaurus Nal: |
germplasm. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01761naa a2200253 a 4500
001 1213888
005 2006-03-20
008 2005 bl uuuu u00u1 u #d
100 1 $aBERED, F.
245 $aGenetic variation among and within sweet corn populations detected by RAPD and SSR markers.
260 $c2005
520 $aThe main objective of the present study was to characterize the genetic structure of three Brazilian sweet corn populations. In addition, we aimed to investigate the molecular genetic diversity within and among the populations and evaluate the ability of RAPD and SSR markers to detect inter and intra-population variation in these populations. PCR were performed using 11 RAPD and six microsatellite primer pairs. Genetic similarity was estimated within and among populations considering the markers independently and jointly. Variability for each locus was measured using the Polymorphism Information Content and the correlation between pairwise distance matrices was obtained. The genetic similarity and analysis of the molecular variance results indicated a higher similarity among than within populations, and RAPD showed lower PIC values than SSR. Knowledge on the genetic diversity distribution in this germplasm is important to determine strategies for future conservation and selection for breeding programs..
650 $agermplasm
650 $aMilho
650 $aZea Mays
653 $aDiversidade genética
653 $aGenetic diversity
653 $aGermoplama
653 $aMarcadores moleculares
653 $aMolecular markers
700 1 $aTERRA, T. de F.
700 1 $aSPELLMEIER, M.
700 1 $aBARBOSA NETO, J. F.
773 $tCrop Breeding and Applied Biotechnology, Viçosa, MG$gv. 5, n. 4, p. 418-425, Dec. 2005.
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Embrapa Arroz e Feijão (CNPAF) |
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