Data/hora: 01/06/2024 09:51:47

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 03/05/2013
Data da última atualização: | 17/01/2024
Autoria: | AVELAR, S. R. G.; MOURA, R. R.; SOUSA, F. C.; PEREIRA, A. F.; ALMEIDA, K. C.; MELO, C. H. S.; TELES-FILHO, A. C. A.; BARIL, G.; MELO, L. M.; TEIXEIRA, D. I. A.; FREITAS, V. J. F.
Título: | Oocyte production and in vitro maturation in Canindé goats following hormonal ovarian stimulation.
Ano de publicação: | 2012
Fonte/Imprenta: | Animal Reproduction, v. 9, n. 1, p. 27-32, Jan./Mar. 2012.
Idioma: | Inglês
Conteúdo: | Abstract: This study evaluated the effect of hormonal ovarian stimulation regimes on the quantity and quality of the cumulus-oocyte complexes (COCs) recovered by laparoscopy and their subsequent in vitro maturation (IVM). Eighteen cyclic Canindé goats received a vaginal sponge with 60 mg medroxyprogesterone acetate for 11 days, together with an injection of 50 ?g d-cloprostenol on the 8th day, along with additional treatment regimens, as follows: i) five doses (5D), 120 mg of NIH-FSH-P1 in five injections at 12 h intervals; ii) three doses (3D), 120 mg of NIH-FSH-P1 in three injections at 24 h intervals; iii) single dose (1D), 70 mg of NIH-FSH-P1 and 200 IU of eCG at 36 h prior to sponge removal. Three sessions of hormonal treatment/oocyte recovery were performed and goats (n = 6/group) were allocated to different groups. The oocytes were collected by laparoscopy at the time of sponge removal and the IVM of the oocytes was monitored. A total of 14.8 ± 0.8 follicles were aspirated per animal with 11.1 ± 0.6 COCs being recovered, resulting in a recovery rate of 74.7% (577/772). The mean number of COCs/goat did not differ (P > 0.05) between treatments (11.7 ± 1.1, 10.7 ± 0.9 and 10.8 ± 1.0 for the 5D, 3D and 1D groups, respectively). Recovery rate was higher (P < 0.05) in the 5D group (84.1%; 211/251) compared to the 3D (68.2%; 182/267) and 1D groups (72.4%; 184/254). The lowest (P < 0.05) maturation rate was recorded in the 3D group (32.1%; 27/84), while the rate for the 5D and 1D groups was 49.1% (53/108) and 46.2% (42/91), respectively. Finally, taking into account the main performance results of the three treatments, it is advisable to use the 5D regime in future Canindé breed preservation programs based on laparoscopy technology as a means to recover oocytes.
Palavras-Chave: | In vitro; Oócito; Raça Canindé.
Thesagro: | Caprino; Gonadotrofina; Hormônio; Maturação; Ovário; Reprodução animal.
Categoria do assunto: | --
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (27179-1) - ADD - AP - DD -  -  -  - DIS