Data/hora: 15/06/2024 07:43:48

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Data corrente: 	27/11/2023	
Data da última atualização: 	14/12/2023	
Autoria: 	SOUZA, S. C. R.; PINHEIRO, R. R.; PEIXOTO, R. M.; SOUSA, A. L. M. de; ANDRIOLI, A.; LIMA, A. M. C.; MENDES, B. K. M.; MAGALHÃES, N. M. de A.; AMARAL, G. P.; TEIXEIRA, M. F. da S.	
Afiliação: 	SAMARA CRISTINA ROCHA SOUZA, STATE UNIVERSITY OF CEARÁ, VIROLOGY LABORATORY (LABOVIR); RAYMUNDO RIZALDO PINHEIRO, CNPC; RENATO MESQUITA PEIXOTO, VALE DO SALGADO UNIVERSITY CENTER (UNIVS); ANA LÍDIA MADEIRA DE SOUSA, FACULDADE EDUCAR DA IBIAPABA; ALICE ANDRIOLI PINHEIRO, CNPC; ANA MILENA CÉSAR LIMA; BÁRBARA KAREN MARQUES MENDES, FEDERAL UNIVERSITY OF PIAUÍ; NATHÁLIA MARIA DE ANDRADE MAGALHÃES, FEDERAL UNIVERSITY OF CAMPINA GRANDE; GABRIEL PAULA AMARAL, VALE DO ACARAÚ STATE UNIVERSITY; MARIA FÁTIMA DA SILVA TEIXEIRA, STATE UNIVERSITY OF CEARÁ, VIROLOGY LABORATORY (LABOVIR).	
Título: 	In vivo evaluation of the antiretroviral activity of Melia azedarach against small ruminant lentiviruses in goat colostrum and milk.	
Ano de publicação: 	2023	
Fonte/Imprenta: 	Brazilian Journal of Microbiology, v. 54, n. 3, Sept. 2023.	
DOI: 	| https://doi.org/10.1038/s41598-023-31455-5	
Idioma: 	Inglês	
Notas: 	On-line first.	
Conteúdo: 	Abstract: This study aimed to evaluate in vivo the use of the extract from the leaves of Melia azedarach in the ethyl acetate fraction at a concentration of 150 µg/mL as an antiretroviral treatment against small ruminant lentiviruses (SRLV) in goat colostrum, and milk with a 90-min action. Two groups of six kids were treated with the extract. One group received three supplies of colostrum from does naturally positive for SRLV, treated with the ethyl acetate fraction of M. azedarach (EAF-MA) for three days, while the other group consumed milk from does also carrying the virus with the respective extract twice a day for five days. After undergoing treatment, all animals began to receive thermized milk until weaning (60 days) and were monitored for six months using nested polymerase chain reaction (nPCR) and western blot (WB) tests. The study revealed cumulative percentages of positive animals in WB or nPCR in the milk group of 66.66% on the seventh day, 83.33% in the following week, and 100% at 120 days, while the colostrum group showed values of 66.66% at 14 days, 83.33% at 90 days, and 100% at 120 days. Variation and intermittency were observed in viral detection, but all animals tested positive in WB or nPCR at some point. A potential delay in infection was observed, which was more significant in the colostrum group. The need for the combination of serological and molecular tests for a more efficient detection of the disease is also emphasized.	
Palavras-Chave: 	Antimicrobial properties; Antiviral action; Antiviral plants; Lactogenic transmission; Natural products; Phytochemistry.	
Thesagro: 	Meliaceae.	
Thesaurus Nal: 	Goat diseases; Goats; Lentivirus; Medicinal plants.	
Categoria do assunto: 	H Saúde e Patologia	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (40688-1) - UPC - AP - DD -  -  -  - DIS