| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
03/05/2013 |
|
Data da última atualização: |
17/01/2024 |
|
Autoria: |
AVELAR, S. R. G.; MOURA, R. R.; SOUSA, F. C.; PEREIRA, A. F.; ALMEIDA, K. C.; MELO, C. H. S.; TELES-FILHO, A. C. A.; BARIL, G.; MELO, L. M.; TEIXEIRA, D. I. A.; FREITAS, V. J. F. |
|
Título: |
Oocyte production and in vitro maturation in Canindé goats following hormonal ovarian stimulation. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
Animal Reproduction, v. 9, n. 1, p. 27-32, Jan./Mar. 2012. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: This study evaluated the effect of hormonal ovarian stimulation regimes on the quantity and quality of the cumulus-oocyte complexes (COCs) recovered by laparoscopy and their subsequent in vitro maturation (IVM). Eighteen cyclic Canindé goats received a vaginal sponge with 60 mg medroxyprogesterone acetate for 11 days, together with an injection of 50 ?g d-cloprostenol on the 8th day, along with additional treatment regimens, as follows: i) five doses (5D), 120 mg of NIH-FSH-P1 in five injections at 12 h intervals; ii) three doses (3D), 120 mg of NIH-FSH-P1 in three injections at 24 h intervals; iii) single dose (1D), 70 mg of NIH-FSH-P1 and 200 IU of eCG at 36 h prior to sponge removal. Three sessions of hormonal treatment/oocyte recovery were performed and goats (n = 6/group) were allocated to different groups. The oocytes were collected by laparoscopy at the time of sponge removal and the IVM of the oocytes was monitored. A total of 14.8 ± 0.8 follicles were aspirated per animal with 11.1 ± 0.6 COCs being recovered, resulting in a recovery rate of 74.7% (577/772). The mean number of COCs/goat did not differ (P > 0.05) between treatments (11.7 ± 1.1, 10.7 ± 0.9 and 10.8 ± 1.0 for the 5D, 3D and 1D groups, respectively). Recovery rate was higher (P < 0.05) in the 5D group (84.1%; 211/251) compared to the 3D (68.2%; 182/267) and 1D groups (72.4%; 184/254). The lowest (P < 0.05) maturation rate was recorded in the 3D group (32.1%; 27/84), while the rate for the 5D and 1D groups was 49.1% (53/108) and 46.2% (42/91), respectively. Finally, taking into account the main performance results of the three treatments, it is advisable to use the 5D regime in future Canindé breed preservation programs based on laparoscopy technology as a means to recover oocytes. MenosAbstract: This study evaluated the effect of hormonal ovarian stimulation regimes on the quantity and quality of the cumulus-oocyte complexes (COCs) recovered by laparoscopy and their subsequent in vitro maturation (IVM). Eighteen cyclic Canindé goats received a vaginal sponge with 60 mg medroxyprogesterone acetate for 11 days, together with an injection of 50 ?g d-cloprostenol on the 8th day, along with additional treatment regimens, as follows: i) five doses (5D), 120 mg of NIH-FSH-P1 in five injections at 12 h intervals; ii) three doses (3D), 120 mg of NIH-FSH-P1 in three injections at 24 h intervals; iii) single dose (1D), 70 mg of NIH-FSH-P1 and 200 IU of eCG at 36 h prior to sponge removal. Three sessions of hormonal treatment/oocyte recovery were performed and goats (n = 6/group) were allocated to different groups. The oocytes were collected by laparoscopy at the time of sponge removal and the IVM of the oocytes was monitored. A total of 14.8 ± 0.8 follicles were aspirated per animal with 11.1 ± 0.6 COCs being recovered, resulting in a recovery rate of 74.7% (577/772). The mean number of COCs/goat did not differ (P > 0.05) between treatments (11.7 ± 1.1, 10.7 ± 0.9 and 10.8 ± 1.0 for the 5D, 3D and 1D groups, respectively). Recovery rate was higher (P < 0.05) in the 5D group (84.1%; 211/251) compared to the 3D (68.2%; 182/267) and 1D groups (72.4%; 184/254). The lowest (P < 0.05) maturation rate was recorded in the 3D group (32.1%; 27/84), while the rate for the 5D an... Mostrar Tudo |
|
Palavras-Chave: |
In vitro; Oócito; Raça Canindé. |
|
Thesagro: |
Caprino; Gonadotrofina; Hormônio; Maturação; Ovário; Reprodução animal. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02771naa a2200349 a 4500
001 1957082
005 2024-01-17
008 2012 bl uuuu u00u1 u #d
100 1 $aAVELAR, S. R. G.
245 $aOocyte production and in vitro maturation in Canindé goats following hormonal ovarian stimulation.$h[electronic resource]
260 $c2012
520 $aAbstract: This study evaluated the effect of hormonal ovarian stimulation regimes on the quantity and quality of the cumulus-oocyte complexes (COCs) recovered by laparoscopy and their subsequent in vitro maturation (IVM). Eighteen cyclic Canindé goats received a vaginal sponge with 60 mg medroxyprogesterone acetate for 11 days, together with an injection of 50 ?g d-cloprostenol on the 8th day, along with additional treatment regimens, as follows: i) five doses (5D), 120 mg of NIH-FSH-P1 in five injections at 12 h intervals; ii) three doses (3D), 120 mg of NIH-FSH-P1 in three injections at 24 h intervals; iii) single dose (1D), 70 mg of NIH-FSH-P1 and 200 IU of eCG at 36 h prior to sponge removal. Three sessions of hormonal treatment/oocyte recovery were performed and goats (n = 6/group) were allocated to different groups. The oocytes were collected by laparoscopy at the time of sponge removal and the IVM of the oocytes was monitored. A total of 14.8 ± 0.8 follicles were aspirated per animal with 11.1 ± 0.6 COCs being recovered, resulting in a recovery rate of 74.7% (577/772). The mean number of COCs/goat did not differ (P > 0.05) between treatments (11.7 ± 1.1, 10.7 ± 0.9 and 10.8 ± 1.0 for the 5D, 3D and 1D groups, respectively). Recovery rate was higher (P < 0.05) in the 5D group (84.1%; 211/251) compared to the 3D (68.2%; 182/267) and 1D groups (72.4%; 184/254). The lowest (P < 0.05) maturation rate was recorded in the 3D group (32.1%; 27/84), while the rate for the 5D and 1D groups was 49.1% (53/108) and 46.2% (42/91), respectively. Finally, taking into account the main performance results of the three treatments, it is advisable to use the 5D regime in future Canindé breed preservation programs based on laparoscopy technology as a means to recover oocytes.
650 $aCaprino
650 $aGonadotrofina
650 $aHormônio
650 $aMaturação
650 $aOvário
650 $aReprodução animal
653 $aIn vitro
653 $aOócito
653 $aRaça Canindé
700 1 $aMOURA, R. R.
700 1 $aSOUSA, F. C.
700 1 $aPEREIRA, A. F.
700 1 $aALMEIDA, K. C.
700 1 $aMELO, C. H. S.
700 1 $aTELES-FILHO, A. C. A.
700 1 $aBARIL, G.
700 1 $aMELO, L. M.
700 1 $aTEIXEIRA, D. I. A.
700 1 $aFREITAS, V. J. F.
773 $tAnimal Reproduction$gv. 9, n. 1, p. 27-32, Jan./Mar. 2012.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
09/01/2018 |
|
Data da última atualização: |
04/04/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
SILVA, T. L. da; GOMES, H. T.; SCHERWINSKI-PEREIRA, J. E. |
|
Afiliação: |
TATIANE LOUREIRO DA SILVA, IFAC; HUGO TEIXEIRA GOMES, UNB; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
|
Título: |
Designing ex-situ conservation strategies for seeds storage of Piper aduncum and P. hispidinervum through cryopreservation and low-temperature techniques. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Journal of Forest Research, v. 22, n. 6, p. 380-385, 2017. |
|
Idioma: |
Português |
|
Palavras-Chave: |
Desiccation. |
|
Thesagro: |
Piperaceae. |
|
Thesaurus NAL: |
Cryopreservation; Germplasm conservation; Vitrification. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/170598/1/Designing-ex-situ-conservation-strategies-for-seeds-storage-of-Piper-aduncum-and-P-hispidinervum-through-cryopreservation-and-low-temperature.pdf
|
|
Marc: |
LEADER 00673naa a2200193 a 4500
001 2084611
005 2023-04-04
008 2017 bl uuuu u00u1 u #d
100 1 $aSILVA, T. L. da
245 $aDesigning ex-situ conservation strategies for seeds storage of Piper aduncum and P. hispidinervum through cryopreservation and low-temperature techniques.$h[electronic resource]
260 $c2017
650 $aCryopreservation
650 $aGermplasm conservation
650 $aVitrification
650 $aPiperaceae
653 $aDesiccation
700 1 $aGOMES, H. T.
700 1 $aSCHERWINSKI-PEREIRA, J. E.
773 $tJournal of Forest Research$gv. 22, n. 6, p. 380-385, 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
