|
|
Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
18/06/2013 |
Data da última atualização: |
08/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PASSOS, M. A. N.; CRUZ, V. O. de; EMEDIATO, F. L.; TEIXEIRA, C. C. de; AZEVEDO, V. C. R.; BRASILEIRO, A. C. M.; AMORIM, E. P.; FERREIRA, C. F.; MARTINS, N. F.; TOGAWA, R. C.; PAPPAS JÚNIOR, G. J.; SILVA JUNIOR, O. B. da; MILLER, R. T N. G. |
Afiliação: |
MARCO A N PASSOS, UNB; VIVIANE OLIVEIRA DE CRUZ, UNB; FLAVIA L EMEDIATO, UNB; CRISTIANE CAMARGO DE TEIXEIRA, UCB; VANIA CRISTINA RENNO AZEVEDO, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; EDSON PERITO AMORIM, CNPMF; CLAUDIA FORTES FERREIRA, CNPMF; NATALIA FLORENCIO MARTINS, CENARGEN; ROBERTO COITI TOGAWA, CENARGEN; GEORGIOS J PAPPAS JÚNIOR, UNB; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; ROBERT N. G. MILLER, UNB. |
Título: |
Analysis of the leaf transcriptome of Musa acuminata during interaction with Mycosphaerella musicola: gene assembly, annotation and marker development. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
BMC Genomics, v.14, n.78, 2013. |
Idioma: |
Inglês |
Conteúdo: |
Background: Although banana (Musa sp.) is an important edible crop, contributing towards poverty alleviation and food security, limited transcriptome datasets are available for use in accelerated molecular-based breeding in this genus. 454 GS-FLX Titanium technology was employed to determine the sequence of gene transcripts in genotypes of Musa acuminata ssp. burmannicoides Calcutta 4 and M. acuminata subgroup Cavendish cv. Grande Naine, contrasting in resistance to the fungal pathogen Mycosphaerella musicola, causal organism of Sigatoka leaf spot disease. To enrich for transcripts under biotic stress responses, full length-enriched cDNA libraries were prepared from whole plant leaf materials, both uninfected and artificially challenged with pathogen conidiospores. Results: The study generated 846,762 high quality sequence reads, with an average length of 334 bp and totaling 283 Mbp. De novo assembly generated 36,384 and 35,269 unigene sequences for M. acuminata Calcutta 4 and Cavendish Grande Naine, respectively. A total of 64.4% of the unigenes were annotated through Basic Local Alignment Search Tool (BLAST) similarity analyses against public databases. Assembled sequences were functionally mapped to Gene Ontology (GO) terms, with unigene functions covering a diverse range of molecular functions, biological processes and cellular components. Genes from a number of defense-related pathways were observed in transcripts from each cDNA library. Over 99% of contig unigenes mapped to exon regions in the reference M. acuminata DH Pahang whole genome sequence. A total of 4068 genic-SSR loci were identified in Calcutta 4 and 4095 in Cavendish Grande Naine. A subset of 95 potential defense-related gene-derived simple sequence repeat (SSR) loci were validated for specific amplification and polymorphism across M. acuminata accessions. Fourteen loci were polymorphic, with alleles per polymorphic locus ranging from 3 to 8 and polymorphism information content ranging from 0.34 to 0.82. Conclusions: A large set of unigenes were characterized in this study for both M. acuminata Calcutta 4 and Cavendish Grande Naine, increasing the number of public domain Musa ESTs. This transcriptome is an invaluable resource for furthering our understanding of biological processes elicited during biotic stresses in Musa. Gene-based markers will facilitate molecular breeding strategies, forming the basis of genetic linkage mapping and analysis of quantitative trait loci. MenosBackground: Although banana (Musa sp.) is an important edible crop, contributing towards poverty alleviation and food security, limited transcriptome datasets are available for use in accelerated molecular-based breeding in this genus. 454 GS-FLX Titanium technology was employed to determine the sequence of gene transcripts in genotypes of Musa acuminata ssp. burmannicoides Calcutta 4 and M. acuminata subgroup Cavendish cv. Grande Naine, contrasting in resistance to the fungal pathogen Mycosphaerella musicola, causal organism of Sigatoka leaf spot disease. To enrich for transcripts under biotic stress responses, full length-enriched cDNA libraries were prepared from whole plant leaf materials, both uninfected and artificially challenged with pathogen conidiospores. Results: The study generated 846,762 high quality sequence reads, with an average length of 334 bp and totaling 283 Mbp. De novo assembly generated 36,384 and 35,269 unigene sequences for M. acuminata Calcutta 4 and Cavendish Grande Naine, respectively. A total of 64.4% of the unigenes were annotated through Basic Local Alignment Search Tool (BLAST) similarity analyses against public databases. Assembled sequences were functionally mapped to Gene Ontology (GO) terms, with unigene functions covering a diverse range of molecular functions, biological processes and cellular components. Genes from a number of defense-related pathways were observed in transcripts from each cDNA library. Over 99% of contig unigenes mapp... Mostrar Tudo |
Thesagro: |
Banana; Fungo; Musa Acuminata; Mycosphaerella Musicola. |
Thesaurus Nal: |
Microsatellite repeats; Transcriptome. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156239/1/1471-2164-14-78.pdf
|
Marc: |
LEADER 03504naa a2200337 a 4500 001 1964212 005 2023-03-08 008 2013 bl uuuu u00u1 u #d 100 1 $aPASSOS, M. A. N. 245 $aAnalysis of the leaf transcriptome of Musa acuminata during interaction with Mycosphaerella musicola$bgene assembly, annotation and marker development.$h[electronic resource] 260 $c2013 520 $aBackground: Although banana (Musa sp.) is an important edible crop, contributing towards poverty alleviation and food security, limited transcriptome datasets are available for use in accelerated molecular-based breeding in this genus. 454 GS-FLX Titanium technology was employed to determine the sequence of gene transcripts in genotypes of Musa acuminata ssp. burmannicoides Calcutta 4 and M. acuminata subgroup Cavendish cv. Grande Naine, contrasting in resistance to the fungal pathogen Mycosphaerella musicola, causal organism of Sigatoka leaf spot disease. To enrich for transcripts under biotic stress responses, full length-enriched cDNA libraries were prepared from whole plant leaf materials, both uninfected and artificially challenged with pathogen conidiospores. Results: The study generated 846,762 high quality sequence reads, with an average length of 334 bp and totaling 283 Mbp. De novo assembly generated 36,384 and 35,269 unigene sequences for M. acuminata Calcutta 4 and Cavendish Grande Naine, respectively. A total of 64.4% of the unigenes were annotated through Basic Local Alignment Search Tool (BLAST) similarity analyses against public databases. Assembled sequences were functionally mapped to Gene Ontology (GO) terms, with unigene functions covering a diverse range of molecular functions, biological processes and cellular components. Genes from a number of defense-related pathways were observed in transcripts from each cDNA library. Over 99% of contig unigenes mapped to exon regions in the reference M. acuminata DH Pahang whole genome sequence. A total of 4068 genic-SSR loci were identified in Calcutta 4 and 4095 in Cavendish Grande Naine. A subset of 95 potential defense-related gene-derived simple sequence repeat (SSR) loci were validated for specific amplification and polymorphism across M. acuminata accessions. Fourteen loci were polymorphic, with alleles per polymorphic locus ranging from 3 to 8 and polymorphism information content ranging from 0.34 to 0.82. Conclusions: A large set of unigenes were characterized in this study for both M. acuminata Calcutta 4 and Cavendish Grande Naine, increasing the number of public domain Musa ESTs. This transcriptome is an invaluable resource for furthering our understanding of biological processes elicited during biotic stresses in Musa. Gene-based markers will facilitate molecular breeding strategies, forming the basis of genetic linkage mapping and analysis of quantitative trait loci. 650 $aMicrosatellite repeats 650 $aTranscriptome 650 $aBanana 650 $aFungo 650 $aMusa Acuminata 650 $aMycosphaerella Musicola 700 1 $aCRUZ, V. O. de 700 1 $aEMEDIATO, F. L. 700 1 $aTEIXEIRA, C. C. de 700 1 $aAZEVEDO, V. C. R. 700 1 $aBRASILEIRO, A. C. M. 700 1 $aAMORIM, E. P. 700 1 $aFERREIRA, C. F. 700 1 $aMARTINS, N. F. 700 1 $aTOGAWA, R. C. 700 1 $aPAPPAS JÚNIOR, G. J. 700 1 $aSILVA JUNIOR, O. B. da 700 1 $aMILLER, R. T N. G. 773 $tBMC Genomics$gv.14, n.78, 2013.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
08/04/2016 |
Data da última atualização: |
08/04/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
RESENDE, M. D. V. de; RAMALHO, M. A. P.; CARNEIRO, P. C. S.; CARNEIRO, J. E. S.; BATISTA, L. G.; GOIS, I. B. |
Afiliação: |
MARCOS DEON VILELA DE RESENDE, CNPF; Magno A. P. Ramalho, UFLA; Pedro C. S. Carneiro, UFV; José E. S. Carneiro, UFV; Lorena G. Batista, UFV; Itamara B. Gois, UFV. |
Título: |
Selection index with parents, populations, progenies, and generations effects in Autogamous plant breeding. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Crop Science, v. 56, n. 2, p. 530-546, Mar./Apr. 2016. |
DOI: |
10.2135/cropsci2015.05.0303 |
Idioma: |
Inglês |
Conteúdo: |
Autogamous plant breeders obtain numerous populations annually, and in the progeny selection process, the merit of the population is not considered. Thus, it would be important to have a progeny selection index that includes not only the effects of progenies in the different generations but also the effects of populations in all the generations and the data from parents and the F1 and F2 generations simultaneously. The main objective of this paper was to develop a selection method that encompasses the entire structure of an autogamous plant breeding program including all the data as of the parents, the F1 and F2 generations, and also both the progeny and population effects in the F3 to F6 generations. To do so, a selection index (called selection index with parents, populations, progenies, and generations [SIPPPG]), which includes all these effects, was proposed. Estimators were also derived for computation of the contribution (through indirect heritabilities and selective accuracies) of each source of information for predicting the additive genetic value of the lines to be obtained at the end of the selection process. The new approach was assessed through numerical evaluation and a total index was also obtained using field data derived from a breeding program with the common bean crop. This new index yields gains in selection efficiency ranging from 5 to 28% depending on the relative magnitude of the genetic variation among populations. |
Palavras-Chave: |
Autogamous plants; Best linear unbiased prediction; BLUP; Generations; Populations; Progenies; Selection index with parents; SIPPPG. |
Thesaurus NAL: |
Plant breeding. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02372naa a2200301 a 4500 001 2042954 005 2016-04-08 008 2016 bl uuuu u00u1 u #d 024 7 $a10.2135/cropsci2015.05.0303$2DOI 100 1 $aRESENDE, M. D. V. de 245 $aSelection index with parents, populations, progenies, and generations effects in Autogamous plant breeding.$h[electronic resource] 260 $c2016 520 $aAutogamous plant breeders obtain numerous populations annually, and in the progeny selection process, the merit of the population is not considered. Thus, it would be important to have a progeny selection index that includes not only the effects of progenies in the different generations but also the effects of populations in all the generations and the data from parents and the F1 and F2 generations simultaneously. The main objective of this paper was to develop a selection method that encompasses the entire structure of an autogamous plant breeding program including all the data as of the parents, the F1 and F2 generations, and also both the progeny and population effects in the F3 to F6 generations. To do so, a selection index (called selection index with parents, populations, progenies, and generations [SIPPPG]), which includes all these effects, was proposed. Estimators were also derived for computation of the contribution (through indirect heritabilities and selective accuracies) of each source of information for predicting the additive genetic value of the lines to be obtained at the end of the selection process. The new approach was assessed through numerical evaluation and a total index was also obtained using field data derived from a breeding program with the common bean crop. This new index yields gains in selection efficiency ranging from 5 to 28% depending on the relative magnitude of the genetic variation among populations. 650 $aPlant breeding 653 $aAutogamous plants 653 $aBest linear unbiased prediction 653 $aBLUP 653 $aGenerations 653 $aPopulations 653 $aProgenies 653 $aSelection index with parents 653 $aSIPPPG 700 1 $aRAMALHO, M. A. P. 700 1 $aCARNEIRO, P. C. S. 700 1 $aCARNEIRO, J. E. S. 700 1 $aBATISTA, L. G. 700 1 $aGOIS, I. B. 773 $tCrop Science$gv. 56, n. 2, p. 530-546, Mar./Apr. 2016.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Florestas (CNPF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|