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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Norte / UEP-Parnaíba. |
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Data corrente: |
18/07/1996 |
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Data da última atualização: |
18/07/1996 |
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Autoria: |
SANTANA, J. M.; TEIXEIRA, A. R. L. |
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Afiliação: |
University of Brasilia. |
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Título: |
Effect of immunotoxins against Trypanosoma cruzi. |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
American Journal of Tropical Medicine and Hygiene, v.41, n.2, p.177-182, 1989. |
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Idioma: |
Inglês |
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Conteúdo: |
Immunotoxins were constructed with IgG antibodies against Trupanosoma cruzi surface antigens by hybridization with abrin (ITA) and ricin (ITR) A chains. The biological activity of the hybrid macromolecules wastested on the parasite forms. Mortility of parasite forms was lost in vitro after incubation with ITR. In general, killing of the parasite with ITR was more efficient than with ITA. Inhibition of protein synthesis after incubation with either ITR or ITA, measured by 3H-leucine incorporation, confirmed the parasite immobilization experiments. The lethal effect was potentiated when the immunotoxins were used in the presence of 2.5 mM ammonium chloride. T. cruzi antibodies specific to cell surface antigens are excellent drug carriers that can be deliveredto the target cell. However, ITR and ITA did not reduce parasitemia orincrease survival of mice infected with T.cruzi. |
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Thesagro: |
Trypanosoma Cruzi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01309naa a2200145 a 4500
001 1077899
005 1996-07-18
008 1989 bl --- 0-- u #d
100 1 $aSANTANA, J. M.
245 $aEffect of immunotoxins against Trypanosoma cruzi.
260 $c1989
520 $aImmunotoxins were constructed with IgG antibodies against Trupanosoma cruzi surface antigens by hybridization with abrin (ITA) and ricin (ITR) A chains. The biological activity of the hybrid macromolecules wastested on the parasite forms. Mortility of parasite forms was lost in vitro after incubation with ITR. In general, killing of the parasite with ITR was more efficient than with ITA. Inhibition of protein synthesis after incubation with either ITR or ITA, measured by 3H-leucine incorporation, confirmed the parasite immobilization experiments. The lethal effect was potentiated when the immunotoxins were used in the presence of 2.5 mM ammonium chloride. T. cruzi antibodies specific to cell surface antigens are excellent drug carriers that can be deliveredto the target cell. However, ITR and ITA did not reduce parasitemia orincrease survival of mice infected with T.cruzi.
650 $aTrypanosoma Cruzi
700 1 $aTEIXEIRA, A. R. L.
773 $tAmerican Journal of Tropical Medicine and Hygiene$gv.41, n.2, p.177-182, 1989.
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Registro original: |
Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP) |
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| Registros recuperados : 2 | |
| 1. |  | BARBOSA, A. S.; ARGAÑARAZ, E. R.; BARROS, A. M.; ROSA, A. de C.; ALVES, N. P.; LOUVANDINI, P.; SOUZA-ALT, M. R. d'; NITZ, N.; STURM, N. R.; NASCIMENTO, R. J.; TEIXEIRA, A. R. L. Hitchhiking Trypanasoma cruzi minicircle DNA affects gene expression in human host cells via LINE-1 retrotransponson. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 8, p. 833-843, Dec. 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Cerrados. |
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| 2. | | BASTOS, I. M. D.; GRELLIER, P.; MARTINS, N. F.; CADAVID-RESTREPO, G.; SOUZA-AULT, M. R. de; AUGUSTYNS, K.; TEIXEIRA, A. R. L.; SCHRÉVEL, J.; MAIGRET, B.; SILVEIRA, J. F. da; SANTANA. J. M. Molecular, functional and structural properties of the prolyl oligopeptidase of Trypanosoma cruzi (POP Tc80), which is required for parasite entry into mammalian cells. Biochemical Journal, v. 388, pt. 1, p. 29-38, may, 2005.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 2 | |
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