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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
06/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROCHA, L. C.; MITTELMANN, A.; HOUBEN, A.; TECHIO, V. H. |
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Afiliação: |
Laiane Corsini Rocha, UFLA; ANDREA MITTELMANN, CNPGL; Andreas Houben, Leibniz Institute of Plant Genetics and Crop Plant Research, Germany; Vânia Helena Techio, UFLA. |
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Título: |
Fragile sites of 45S rDNA of Lolium multiflorum are not hotspots for chromosomal breakages induced by X-ray. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 43, n. 7, p. 659-665, 2016. |
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Idioma: |
Português |
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Conteúdo: |
Abstract Sites of 45S rDNA of Lolium are regions denominated fragile sites (FSs), constituting regions slightly stained with DAPI due to increased DNA unpacking in metaphasic chromosomes. Considered to be fragile regions in the genome, the FSs might be more responsive to induced breaks and result in chromosomal fragments and rearrangements, unless repairing mechanisms such as recombination or de novo telomere formation play a role at the break site of the DNA. Thus, this study aimed at investigating if SFs from Lolium are hotspots for the occurrence of breakages induced by X-ray and if they are regions favorable to synthesize new telomeres, using Hordeum vulgare as a comparative model. Lolium multiflorum and H. vulgare seedlings were irradiated with 20 and 50 Gy X-ray and evaluated one day following the irradiation and at 7-days intervals for a 28-days period, using FISH technique with 45S rDNA and Arabidopsistype telomere probes in order to investigate the presence of chromosomal breakages and new telomere formation. H. vulgare did not survive after a few days of irradiation due to the increased rate of abnormalities. L. multiflorum also exhibited chromosomal abnormalities following the exposure, yet over the 28-days trial it had a decrease in the chromosomal damage rate and formation of de novo telomere has not been detected along this time. Despite being considered to be fragile regions in the genome, the 45S rDNA sites of Lolium are not hotspots to chromosomal breakages after the induction of breakages. MenosAbstract Sites of 45S rDNA of Lolium are regions denominated fragile sites (FSs), constituting regions slightly stained with DAPI due to increased DNA unpacking in metaphasic chromosomes. Considered to be fragile regions in the genome, the FSs might be more responsive to induced breaks and result in chromosomal fragments and rearrangements, unless repairing mechanisms such as recombination or de novo telomere formation play a role at the break site of the DNA. Thus, this study aimed at investigating if SFs from Lolium are hotspots for the occurrence of breakages induced by X-ray and if they are regions favorable to synthesize new telomeres, using Hordeum vulgare as a comparative model. Lolium multiflorum and H. vulgare seedlings were irradiated with 20 and 50 Gy X-ray and evaluated one day following the irradiation and at 7-days intervals for a 28-days period, using FISH technique with 45S rDNA and Arabidopsistype telomere probes in order to investigate the presence of chromosomal breakages and new telomere formation. H. vulgare did not survive after a few days of irradiation due to the increased rate of abnormalities. L. multiflorum also exhibited chromosomal abnormalities following the exposure, yet over the 28-days trial it had a decrease in the chromosomal damage rate and formation of de novo telomere has not been detected along this time. Despite being considered to be fragile regions in the genome, the 45S rDNA sites of Lolium are not hotspots to chromosomal breakages ... Mostrar Tudo |
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Palavras-Chave: |
Chromosomal abnormalities; De novo telomere formation; Ryegrass. |
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Thesaurus Nal: |
barley; DNA repair; ionizing radiation. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02226naa a2200229 a 4500
001 2062871
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aROCHA, L. C.
245 $aFragile sites of 45S rDNA of Lolium multiflorum are not hotspots for chromosomal breakages induced by X-ray.$h[electronic resource]
260 $c2016
520 $aAbstract Sites of 45S rDNA of Lolium are regions denominated fragile sites (FSs), constituting regions slightly stained with DAPI due to increased DNA unpacking in metaphasic chromosomes. Considered to be fragile regions in the genome, the FSs might be more responsive to induced breaks and result in chromosomal fragments and rearrangements, unless repairing mechanisms such as recombination or de novo telomere formation play a role at the break site of the DNA. Thus, this study aimed at investigating if SFs from Lolium are hotspots for the occurrence of breakages induced by X-ray and if they are regions favorable to synthesize new telomeres, using Hordeum vulgare as a comparative model. Lolium multiflorum and H. vulgare seedlings were irradiated with 20 and 50 Gy X-ray and evaluated one day following the irradiation and at 7-days intervals for a 28-days period, using FISH technique with 45S rDNA and Arabidopsistype telomere probes in order to investigate the presence of chromosomal breakages and new telomere formation. H. vulgare did not survive after a few days of irradiation due to the increased rate of abnormalities. L. multiflorum also exhibited chromosomal abnormalities following the exposure, yet over the 28-days trial it had a decrease in the chromosomal damage rate and formation of de novo telomere has not been detected along this time. Despite being considered to be fragile regions in the genome, the 45S rDNA sites of Lolium are not hotspots to chromosomal breakages after the induction of breakages.
650 $abarley
650 $aDNA repair
650 $aionizing radiation
653 $aChromosomal abnormalities
653 $aDe novo telomere formation
653 $aRyegrass
700 1 $aMITTELMANN, A.
700 1 $aHOUBEN, A.
700 1 $aTECHIO, V. H.
773 $tMolecular Biology Reports$gv. 43, n. 7, p. 659-665, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
19/12/2017 |
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Data da última atualização: |
21/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
REIS, A. N. dos; MIRANDA, M. dos S.; SILVA, L. K. X.; SILVA, A. O. A. da; SOUSA, J. S. de; MORAIS, E. de; GARCIA, A. R.; DOMINGUES, S. F. S.; SILVA, J. K. do R. da; RIBEIRO, H. F. L. |
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Afiliação: |
Adriana Novaes dos Reis, UFPA; Moysés dos Santos Miranda, UFPA; Lílian Kátia Ximenes Silva, UNAMA; Aluizio Otavio Almeida da Silva, UFPA; José Silva de Sousa, UFPA; Eziquiel de Morais, IFPA; ALEXANDRE ROSSETTO GARCIA, CPPSE; Sheyla Farhayldes Souza Domingues, UFPA; Joyce Kelly do Rosário da Silva, UFPA; Haroldo Francisco Lobato Ribeiro, UFRA. |
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Título: |
Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 38, n. 6, p. 3613-3628, 2017. |
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DOI: |
10.5433/1679-0359.2017v38n6p3613 |
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Idioma: |
Inglês |
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Conteúdo: |
For artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil. MenosFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evalu... Mostrar Tudo |
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Palavras-Chave: |
Powdered Coconut Water. |
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Thesaurus NAL: |
cryopreservation; Lippia. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169304/1/REIS-et-al-2016-Comparative-Evaluation-Between-TES-TRIS-and-ACP-112.pdf
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Marc: |
LEADER 03425naa a2200277 a 4500
001 2082949
005 2019-05-21
008 2017 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2017v38n6p3613$2DOI
100 1 $aREIS, A. N. dos
245 $aComparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm.$h[electronic resource]
260 $c2017
520 $aFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil.
650 $acryopreservation
650 $aLippia
653 $aPowdered Coconut Water
700 1 $aMIRANDA, M. dos S.
700 1 $aSILVA, L. K. X.
700 1 $aSILVA, A. O. A. da
700 1 $aSOUSA, J. S. de
700 1 $aMORAIS, E. de
700 1 $aGARCIA, A. R.
700 1 $aDOMINGUES, S. F. S.
700 1 $aSILVA, J. K. do R. da
700 1 $aRIBEIRO, H. F. L.
773 $tSemina: Ciências Agrárias, Londrina$gv. 38, n. 6, p. 3613-3628, 2017.
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Embrapa Pecuária Sudeste (CPPSE) |
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