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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
02/12/2011 |
Data da última atualização: |
02/12/2011 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DIAS, A. C. F.; DINI-ANDREOTE, F.; TAKETANI, R. G.; TSAI, S. M.; AZEVEDO, J. L.; MELO, I. S. de; ANDREOTE, F. D. |
Afiliação: |
ARMANDO CAVALCANTE FRANCO DIAS, CENA-USP; FRANCISCO DINI-ANDREOTE, ESALQ-USP; RODIRGO GOUVÊA TAKETANI, CENA-USP; SIU MUI TSAI, CENA-USP; JOÃO LÚCIO AZEVEDO, CENA-USP; ITAMAR SOARES DE MELO, CNPMA; FERNANDO DINI ANDREOTE, ESALQ-USP. |
Título: |
Archaeal communities in the sediments of three contrasting mangroves. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Journal of Soils and Sediments, Berlin, v. 11, n. 8, p. 1466-1476, 2011. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study evaluates the presence and diversity of 16S rRNA (rrs) and amoA genes from archaea in three mangrove sediments under different stages of preservation(one pristine mangrove, one affected by anthropogenic activity, and another contaminated by an oil spill) in the state of São Paulo (Brazil). Materials and methods A combination of DGGE, coupled with ordination analysis, and clone libraries of both targeted genes (rrs and amoA) was used to infer the diversity and phylogeny of archaeal communities in the mangrove analyzed samples. Results and discussion The DGGE combined with multivariate analysis revealed, based on the ribosomal gene, differences in archaeal communities according to environmental parameters such as mangrove location, anthropogenic activity, and oil contamination. The profiles based on the amoA gene were more similar than those obtained for the gene rrs, with the only difference statistically observed for the community found in the mangrove under anthropogenic pressure. Furthermore, phylogenetic analysis revealed most archaeal groups affiliated to sequences belonging to the Thaumarchaeota (53.1%, 24 OTUs) and Euryarchaeota (29.6%, 14 OTUs) phyla, in addition to 14 sequences affiliated to an unclassified Archaea (16.1%, 8 OTUs). Concerning the analysis of the amoA gene, mangroves harbored sequences affiliated with those previously described in water column and soil/sediment samples, besides two possible clusters specifically found in mangroves. Conclusions The findings are that the mangroves act as a reservoir for archaeal diversity, are possibly involved in nitrogen transformation in this ecosystem, and are affected by distinct pressures caused by anthropogenic activities. MenosAbstract: This study evaluates the presence and diversity of 16S rRNA (rrs) and amoA genes from archaea in three mangrove sediments under different stages of preservation(one pristine mangrove, one affected by anthropogenic activity, and another contaminated by an oil spill) in the state of São Paulo (Brazil). Materials and methods A combination of DGGE, coupled with ordination analysis, and clone libraries of both targeted genes (rrs and amoA) was used to infer the diversity and phylogeny of archaeal communities in the mangrove analyzed samples. Results and discussion The DGGE combined with multivariate analysis revealed, based on the ribosomal gene, differences in archaeal communities according to environmental parameters such as mangrove location, anthropogenic activity, and oil contamination. The profiles based on the amoA gene were more similar than those obtained for the gene rrs, with the only difference statistically observed for the community found in the mangrove under anthropogenic pressure. Furthermore, phylogenetic analysis revealed most archaeal groups affiliated to sequences belonging to the Thaumarchaeota (53.1%, 24 OTUs) and Euryarchaeota (29.6%, 14 OTUs) phyla, in addition to 14 sequences affiliated to an unclassified Archaea (16.1%, 8 OTUs). Concerning the analysis of the amoA gene, mangroves harbored sequences affiliated with those previously described in water column and soil/sediment samples, besides two possible clusters specifically found in mangroves... Mostrar Tudo |
Palavras-Chave: |
16S rRNA; Ammonium processing; amoA; Thaumarchaeota. |
Thesagro: |
Mangue; Microrganismo; Sedimento. |
Thesaurus Nal: |
Archaea; Euryarchaeota; Mangrove swamps. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02590naa a2200313 a 4500 001 1908298 005 2011-12-02 008 2011 bl uuuu u00u1 u #d 100 1 $aDIAS, A. C. F. 245 $aArchaeal communities in the sediments of three contrasting mangroves.$h[electronic resource] 260 $c2011 520 $aAbstract: This study evaluates the presence and diversity of 16S rRNA (rrs) and amoA genes from archaea in three mangrove sediments under different stages of preservation(one pristine mangrove, one affected by anthropogenic activity, and another contaminated by an oil spill) in the state of São Paulo (Brazil). Materials and methods A combination of DGGE, coupled with ordination analysis, and clone libraries of both targeted genes (rrs and amoA) was used to infer the diversity and phylogeny of archaeal communities in the mangrove analyzed samples. Results and discussion The DGGE combined with multivariate analysis revealed, based on the ribosomal gene, differences in archaeal communities according to environmental parameters such as mangrove location, anthropogenic activity, and oil contamination. The profiles based on the amoA gene were more similar than those obtained for the gene rrs, with the only difference statistically observed for the community found in the mangrove under anthropogenic pressure. Furthermore, phylogenetic analysis revealed most archaeal groups affiliated to sequences belonging to the Thaumarchaeota (53.1%, 24 OTUs) and Euryarchaeota (29.6%, 14 OTUs) phyla, in addition to 14 sequences affiliated to an unclassified Archaea (16.1%, 8 OTUs). Concerning the analysis of the amoA gene, mangroves harbored sequences affiliated with those previously described in water column and soil/sediment samples, besides two possible clusters specifically found in mangroves. Conclusions The findings are that the mangroves act as a reservoir for archaeal diversity, are possibly involved in nitrogen transformation in this ecosystem, and are affected by distinct pressures caused by anthropogenic activities. 650 $aArchaea 650 $aEuryarchaeota 650 $aMangrove swamps 650 $aMangue 650 $aMicrorganismo 650 $aSedimento 653 $a16S rRNA 653 $aAmmonium processing 653 $aamoA 653 $aThaumarchaeota 700 1 $aDINI-ANDREOTE, F. 700 1 $aTAKETANI, R. G. 700 1 $aTSAI, S. M. 700 1 $aAZEVEDO, J. L. 700 1 $aMELO, I. S. de 700 1 $aANDREOTE, F. D. 773 $tJournal of Soils and Sediments, Berlin$gv. 11, n. 8, p. 1466-1476, 2011.
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Embrapa Meio Ambiente (CNPMA) |
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Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
28/01/2009 |
Data da última atualização: |
28/01/2009 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
CASTRO, A. M. G. de; LIMA, S. M. V.; KOURI, J.; SILVA, M. de F. M. M. da; CARTAXO, W. V. |
Afiliação: |
Antônio Maria Gomes de Castro, Embrapa DPD; Suzana Maria Valle Lima, Embrapa DPD; Joffre Kouri, Embrapa Algodão; Maria de Fátima Marchezan Menezes da Silva, Embrapa DPD; Waltemilton Vieira Cartaxo, Embrapa Algodão. |
Título: |
Viabilidade, competitividade e sustentabilidade da cadeia produtiva de mamona na obtenção de biocombustíveis. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MAMONA, 3., 2008, Salvador. Energia e ricinoquímica: anais. Salvador: SEAGRI: Embrapa Algodão, 2008. |
Descrição Física: |
1 CD-ROM |
Idioma: |
Português |
Palavras-Chave: |
Processo produtivo; Segmentação. |
Thesagro: |
Cadeia Produtiva; Mamona. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPA-2009-09/21934/1/E-02.pdf
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Marc: |
LEADER 00708nam a2200205 a 4500 001 1276984 005 2009-01-28 008 2008 bl uuuu u00u1 u #d 100 1 $aCASTRO, A. M. G. de 245 $aViabilidade, competitividade e sustentabilidade da cadeia produtiva de mamona na obtenção de biocombustíveis. 260 $aIn: CONGRESSO BRASILEIRO DE MAMONA, 3., 2008, Salvador. Energia e ricinoquímica: anais. Salvador: SEAGRI: Embrapa Algodão$c2008 300 $c1 CD-ROM 650 $aCadeia Produtiva 650 $aMamona 653 $aProcesso produtivo 653 $aSegmentação 700 1 $aLIMA, S. M. V. 700 1 $aKOURI, J. 700 1 $aSILVA, M. de F. M. M. da 700 1 $aCARTAXO, W. V.
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