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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
19/02/2016 |
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Data da última atualização: |
19/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TAKETANI, R. G.; MOITINHO, M. A.; MELO, I. S. de. |
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Afiliação: |
R. G. TAKETANI, Bolsista FAPESP; M. A. MOITINHO, Bolsista CNPq; ITAMAR SOARES DE MELO, CNPMA. |
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Título: |
Metatranscriptomic and Metagenomic insights into the litter decomposition in mangroves. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1749-1. |
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Idioma: |
Português |
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Conteúdo: |
Mangroves are costal ecosystems known for its important role as sink and source of organic matter. This ecosystem covers most of the tropical and subtropical shores. A major portion of the organic matter that is produced by the mangrove vegetation is exported to nearby marine ecosystems. Therefore, this study was designed to shed light over the process of litter decomposition by using high throughput sequencing of the mRNA and DNA present in decaying mangrove leaves. With this purpose, litter bags contatining leaves of Laguncularia racemosa, Avicennia schaueriana and Rhizophora mangle were left on the mangrove sediment for 60 days. This experiment was conducted in three different mangroves in different locations. These material was had its total DNA and mRNA extracted and used to construct libraries that were sequenced in an ion torrent PGM. Sequences were annotated by MG-RAST, CAZY and Crass. The community patterns were very similar between plants; however, there were significant differences between locations. There were some important differences between the groups observed for in the DNA and mRNA libraries. The major phylogenetic groups observed were Gamma and Deltaproteobacteria while the functions were clustering-based subsystems, carbohydrate, amino acid and derivatives, protein, miscellaneous metabolism. The search for carbohydrate metabolism genes using CAZY found genes related to plant organic matter decomposition (GH26, GH17, AA3 and GH43). The major difference was a switch between Gamma and Deltaproteobacteria as dominant classes between mRNA and DNA (respectively). Another striking difference was that the DNA libraries were much more similar, despite the geographical distance than the mRNA. Hits findings highlight the importance of environmental signals to the expression of genes. MenosMangroves are costal ecosystems known for its important role as sink and source of organic matter. This ecosystem covers most of the tropical and subtropical shores. A major portion of the organic matter that is produced by the mangrove vegetation is exported to nearby marine ecosystems. Therefore, this study was designed to shed light over the process of litter decomposition by using high throughput sequencing of the mRNA and DNA present in decaying mangrove leaves. With this purpose, litter bags contatining leaves of Laguncularia racemosa, Avicennia schaueriana and Rhizophora mangle were left on the mangrove sediment for 60 days. This experiment was conducted in three different mangroves in different locations. These material was had its total DNA and mRNA extracted and used to construct libraries that were sequenced in an ion torrent PGM. Sequences were annotated by MG-RAST, CAZY and Crass. The community patterns were very similar between plants; however, there were significant differences between locations. There were some important differences between the groups observed for in the DNA and mRNA libraries. The major phylogenetic groups observed were Gamma and Deltaproteobacteria while the functions were clustering-based subsystems, carbohydrate, amino acid and derivatives, protein, miscellaneous metabolism. The search for carbohydrate metabolism genes using CAZY found genes related to plant organic matter decomposition (GH26, GH17, AA3 and GH43). The major difference was... Mostrar Tudo |
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Palavras-Chave: |
Mangrove; Metagenomic; Metatranscriptomic. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139532/1/2015RA-014.pdf
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Marc: |
LEADER 02441nam a2200169 a 4500
001 2037826
005 2016-02-19
008 2015 bl uuuu u00u1 u #d
100 1 $aTAKETANI, R. G.
245 $aMetatranscriptomic and Metagenomic insights into the litter decomposition in mangroves.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1749-1.$c1749
520 $aMangroves are costal ecosystems known for its important role as sink and source of organic matter. This ecosystem covers most of the tropical and subtropical shores. A major portion of the organic matter that is produced by the mangrove vegetation is exported to nearby marine ecosystems. Therefore, this study was designed to shed light over the process of litter decomposition by using high throughput sequencing of the mRNA and DNA present in decaying mangrove leaves. With this purpose, litter bags contatining leaves of Laguncularia racemosa, Avicennia schaueriana and Rhizophora mangle were left on the mangrove sediment for 60 days. This experiment was conducted in three different mangroves in different locations. These material was had its total DNA and mRNA extracted and used to construct libraries that were sequenced in an ion torrent PGM. Sequences were annotated by MG-RAST, CAZY and Crass. The community patterns were very similar between plants; however, there were significant differences between locations. There were some important differences between the groups observed for in the DNA and mRNA libraries. The major phylogenetic groups observed were Gamma and Deltaproteobacteria while the functions were clustering-based subsystems, carbohydrate, amino acid and derivatives, protein, miscellaneous metabolism. The search for carbohydrate metabolism genes using CAZY found genes related to plant organic matter decomposition (GH26, GH17, AA3 and GH43). The major difference was a switch between Gamma and Deltaproteobacteria as dominant classes between mRNA and DNA (respectively). Another striking difference was that the DNA libraries were much more similar, despite the geographical distance than the mRNA. Hits findings highlight the importance of environmental signals to the expression of genes.
653 $aMangrove
653 $aMetagenomic
653 $aMetatranscriptomic
700 1 $aMOITINHO, M. A.
700 1 $aMELO, I. S. de
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
19/04/2010 |
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Data da última atualização: |
19/04/2010 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
SICILIANO, I.; SILVA, E. M. M.; SILVA, J. B. C.; RAMOS, S. R. R.; DELIZA, R.; CARVALHO, J. L. V.; NUTTI, M. |
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Afiliação: |
ISABEL SICILIANO, UNIRIO; E. M. M. SILVA, UFRRJ; JOÃO BOSCO CARVALHO DA SILVA, CNPH; SEMIRAMIS RABELO RAMALHO RAMOS, CPATC; ROSIRES DELIZA, Labex Europa; JOSE LUIZ VIANA DE CARVALHO, CTAA; MARILIA REGINI NUTTI, CTAA. |
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Título: |
Preferência de biscoitos elaborados com farinha de batata-doce de polpa alaranjada e biscoitos com farinha de abóbora. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PROCESSAMENTO DE FRUTAS E HORTALIÇAS, 1., 2009, Rio de Janeiro. Alimentação, qualidade de vida e auto-sustentabilidade: anais... Rio de Janeiro: SBPFH, 2009. 1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Farinha de abóbora; Farinha de batata-doce de polpa alaranjada; Preferência do consumidor. |
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Thesagro: |
Biscoito; Saúde; Vitamina A. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 00922naa a2200253 a 4500
001 1696846
005 2010-04-19
008 2009 bl --- 0-- u #d
100 1 $aSICILIANO, I.
245 $aPreferência de biscoitos elaborados com farinha de batata-doce de polpa alaranjada e biscoitos com farinha de abóbora.
260 $c2009
650 $aBiscoito
650 $aSaúde
650 $aVitamina A
653 $aFarinha de abóbora
653 $aFarinha de batata-doce de polpa alaranjada
653 $aPreferência do consumidor
700 1 $aSILVA, E. M. M.
700 1 $aSILVA, J. B. C.
700 1 $aRAMOS, S. R. R.
700 1 $aDELIZA, R.
700 1 $aCARVALHO, J. L. V.
700 1 $aNUTTI, M.
773 $tIn: CONGRESSO BRASILEIRO DE PROCESSAMENTO DE FRUTAS E HORTALIÇAS, 1., 2009, Rio de Janeiro. Alimentação, qualidade de vida e auto-sustentabilidade: anais... Rio de Janeiro: SBPFH, 2009. 1 CD-ROM.
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Embrapa Agroindústria de Alimentos (CTAA) |
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