Data/hora: 03/06/2024 08:00:53

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Data corrente: 	30/08/2002	
Data da última atualização: 	26/01/2024	
Autoria: 	MAUDET, C.; TABERLET, P.	
Título: 	Detection of cows' milk in goats' cheeses inferred from mitochondrial DNA polymorphism.	
Ano de publicação: 	2001	
Fonte/Imprenta: 	Journal of Dairey Research, v. 68, n. 2, p. 229-235, May, 2001.	
DOI: 	https://doi.org/10.1017/S0022029901004794	
Idioma: 	Inglês	
Conteúdo: 	Abstract: A new polymerase chain reaction (PCR)-based method was developed to detect cows' milk in goat cheese. This method is based on mitochondrial DNA (mtDNA) control region sequence variations. DNA extractions from 150 mg of cheese were carried out using a spin column-based method. Subsequent PCR amplifications of DNA were performed with cow specific primers, demonstrating the ability to detect cows' milk in a variety of cheeses. This simple approach provides high quality DNA, and is shown to be very sensitive, with a detection limit of less than 0·1% of cows' milk. Analysis of an agarose gel digital image allows a rough estimation of the percentage of cows' milk used in adulteration.	
Palavras-Chave: 	Cow milk; Detecção; Deteccao em PCR; DNA Mitochondrial; DNA mitocondrial; Genética mitocondrial; Leite de Vaca; PCR.	
Thesagro: 	Bovino; Caprino; Citogenética Animal; DNA; Leite de Cabra; Polimorfismo; Queijo.	
Thesaurus Nal: 	Adulterated products; Cattle; Cheese milk; Cheeses; Detection; Food analysis; Food chemistry; Food contamination; Genetic polymorphism; Goat cheese; Goat milk; Goats; Milk proteins; Mitochondrial DNA; Polymerase chain reaction.	
Categoria do assunto: 	K Ciência Florestal e Produtos de Origem Vegetal
L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (22208-1) - ADD - AP - DD -  -  -  - DIS
CNPC - (14826-1) - ADD - AP - DD -  -  -  - DIS