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 | Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
06/12/2023 |
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Data da última atualização: |
06/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
IVAMOTO-SUZUKI, S. T.; CELEDÓN, J. M.; YUEN, M. M. S.; KITZBERGER, C. S. G.; DOMINGUES, D. S.; BOHLMANN, J.; PEREIRA, L. F. P. |
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Afiliação: |
SUZANA TIEMI IVAMOTO-SUZUKI, UNIVERSIDADE ESTADUAL DE LONDRINA; JOSÉ MIGUEL CELEDÓN, UNIVERSITY OF BRITISH COLUMBIA; MACAIRE M. S. YUEN, UNIVERSITY OF BRITISH COLUMBIA; CÍNTIA SORANE GOOD KITZBERGER, INSTITUTO DE DESENVOLVIMENTO RURAL DO PARANÁ; DOUGLAS SILVA DOMINGUES, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; JÖRG BOHLMANN, UNIVERSITY OF BRITISH COLUMBIA; LUIZ FILIPE PROTASIO PEREIRA, CNPCa. |
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Título: |
Functional characterization of ent-copalyl diphosphate synthase and kaurene synthase genes from coffea arábica L. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, v. 71, n. 42, p. 15863-15873, 2023. |
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Idioma: |
Inglês |
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Conteúdo: |
The biochemical profile of coffee beans translates directly into quality traits, nutraceutical and health promoting properties of the coffee beverage. Ent-kaurene is the ubiquitous precursor for gibberellin biosynthesis in plants, but it also serves as an intermediate in specialized (i.e., secondary) diterpenoid metabolism that leads to a diversity of more than 1,000 different metabolites. Nutraceutical effects on human health attributed to diterpenes include antioxidant, anticarcinogenic, and anti-inflammatory properties. Cafestol (CAF) and kahweol (KAH) are two diterpenes found exclusively in the Coffea genus. Our objective was to identify and functionally characterize genes involved in the central step of ent-kaurene production. We identified 17 putative terpene synthase genes in the transcriptome of Coffea arabica. Two ent-copalyl diphosphate synthase (CaCPS) and three kaurene synthase (CaKS) were selected and manually annotated. Transcript expression profiles of CaCPS1 and CaKS3 best matched the CAF and KAH metabolite profiles in different tissues. CaCPS1 and CaKS3 proteins were heterologously expressed and functionally characterized. CaCPS1 catalyzes the cyclization of geranylgeranyl diphosphate (GGPP) to ent-copalyl diphosphate (ent-CPP), which is converted to ent-kaurene by CaKS3. Knowledge about the central steps of diterpene formation in coffee provides a foundation for future characterization of the subsequent enzymes involved in CAF and KAH biosynthesis. |
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Thesaurus Nal: |
Bioinformatics; Biosynthesis; Caffeine; Coffee beans; Enzymes; Gene expression. |
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Categoria do assunto: |
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Marc: |
LEADER 02304naa a2200265 a 4500
001 2159212
005 2023-12-06
008 2023 bl uuuu u00u1 u #d
100 1 $aIVAMOTO-SUZUKI, S. T.
245 $aFunctional characterization of ent-copalyl diphosphate synthase and kaurene synthase genes from coffea arábica L.$h[electronic resource]
260 $c2023
520 $aThe biochemical profile of coffee beans translates directly into quality traits, nutraceutical and health promoting properties of the coffee beverage. Ent-kaurene is the ubiquitous precursor for gibberellin biosynthesis in plants, but it also serves as an intermediate in specialized (i.e., secondary) diterpenoid metabolism that leads to a diversity of more than 1,000 different metabolites. Nutraceutical effects on human health attributed to diterpenes include antioxidant, anticarcinogenic, and anti-inflammatory properties. Cafestol (CAF) and kahweol (KAH) are two diterpenes found exclusively in the Coffea genus. Our objective was to identify and functionally characterize genes involved in the central step of ent-kaurene production. We identified 17 putative terpene synthase genes in the transcriptome of Coffea arabica. Two ent-copalyl diphosphate synthase (CaCPS) and three kaurene synthase (CaKS) were selected and manually annotated. Transcript expression profiles of CaCPS1 and CaKS3 best matched the CAF and KAH metabolite profiles in different tissues. CaCPS1 and CaKS3 proteins were heterologously expressed and functionally characterized. CaCPS1 catalyzes the cyclization of geranylgeranyl diphosphate (GGPP) to ent-copalyl diphosphate (ent-CPP), which is converted to ent-kaurene by CaKS3. Knowledge about the central steps of diterpene formation in coffee provides a foundation for future characterization of the subsequent enzymes involved in CAF and KAH biosynthesis.
650 $aBioinformatics
650 $aBiosynthesis
650 $aCaffeine
650 $aCoffee beans
650 $aEnzymes
650 $aGene expression
700 1 $aCELEDÓN, J. M.
700 1 $aYUEN, M. M. S.
700 1 $aKITZBERGER, C. S. G.
700 1 $aDOMINGUES, D. S.
700 1 $aBOHLMANN, J.
700 1 $aPEREIRA, L. F. P.
773 $tJournal of Agricultural and Food Chemistry$gv. 71, n. 42, p. 15863-15873, 2023.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Algodão. Para informações adicionais entre em contato com cnpa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
28/01/2009 |
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Data da última atualização: |
28/01/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ALVES, M. F.; SILVA, U. C. da; BARROSO, P. A. V.; HOFFMANN, L. V. |
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Afiliação: |
Milena Ferreira Alves, estagiária; Uiara Cavalcante da Silva, estagiária; Paulo Augusto Vianna Barroso, Embrapa Algodão; Lúcia Vieira Hoffmann, Embrapa Algodão. |
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Título: |
Índice de ataque de rosada (pectinophora gossypiella) em Gossypium mustelinum em ambiente natural. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE BOTÂNICA, 59.; REUNIÃO NORDESTINA DE BOTÂNICA, 31.; CONGRESSO LATINOAMERICANO Y DEL CARIBE DE CACTÁCEAS Y OTRAS SUCULENTAS, 4.; CONGRESS OF INTERNATIONAL ORGANIZATION FOR SUCULENT PLANT STUDY, 30., 2008, Natal. Atualidades, desafios e perspectivas da botânica no Brasil: resumos. Natal: UFERSA: UFRN: SBB, 2008. |
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Idioma: |
Português |
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Palavras-Chave: |
Bacia do Rio Paraguaçu; Gossypium mustelinum; Pectinophora gossypiell. |
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Categoria do assunto: |
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Marc: |
LEADER 00862naa a2200181 a 4500
001 1277265
005 2009-01-28
008 2008 bl --- 0-- u #d
100 1 $aALVES, M. F.
245 $aÍndice de ataque de rosada (pectinophora gossypiella) em Gossypium mustelinum em ambiente natural.
260 $c2008
653 $aBacia do Rio Paraguaçu
653 $aGossypium mustelinum
653 $aPectinophora gossypiell
700 1 $aSILVA, U. C. da
700 1 $aBARROSO, P. A. V.
700 1 $aHOFFMANN, L. V.
773 $tIn: CONGRESSO NACIONAL DE BOTÂNICA, 59.; REUNIÃO NORDESTINA DE BOTÂNICA, 31.; CONGRESSO LATINOAMERICANO Y DEL CARIBE DE CACTÁCEAS Y OTRAS SUCULENTAS, 4.; CONGRESS OF INTERNATIONAL ORGANIZATION FOR SUCULENT PLANT STUDY, 30., 2008, Natal. Atualidades, desafios e perspectivas da botânica no Brasil: resumos. Natal: UFERSA: UFRN: SBB, 2008.
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