Data/hora: 07/06/2024 18:24:11

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 20/05/1993
Data da última atualização: | 20/05/1993
Autoria: | SU H.; LIN, C.
Afiliação: | Department of Plant Pathology & Entomology, National Taiwan University, Taipei, Taiwan, China.
Título: | Yolk media for cultivation of spiroplasmas.
Ano de publicação: | 1981
Fonte/Imprenta: | Tokyo, Japan: International Society of Citricultural, 1981.
Volume: | v.1
Páginas: | p.458-460
Idioma: | Inglês
Conteúdo: | Citrus stubborn spiroplasma (S. citri) and honeybee spiroplasma grew favorably in C-3G basic medium containing 5% yolk fluid or 10% amniotic fluid. S. citri, honeybee spiroplasma (HB-24), and flower spiroplasma (SR-3) grew favorably in yolk media formulated by replacing the horse serum constituent of commonly used serum media with 0.6-5.0% chiken egg yolk fluid. The serum constituent in serum media could be substituted by yolk, but yolk could not substitute for PPLO broth or sucrose. Yolk solution clarified by heating at 56 graus for 30 min, centrifuging at 10,000 rpm for 30 min, and membrane (0.45 micron) filtering, was slightly better for growth than unclarified yolk solution which hindered microscopic observation. S. citri grew better in a media with low yolk content (1.25-0.65%), while HB-24 and corn-stunt spiroplasmas grew better in 2.5-5.0% yolk medium formulated with phosphate buffer solution (0.01M) at pH 7.2-7.6. SR-3 grew equally well in any medium containing over 0.65% yolk. The yolk media were much cheaper than serum media.
Palavras-Chave: | Flower spiroplasma; Honeybee spiroplasma; Stubborn.
Thesaurus Nal: | Spiroplasma citri.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (13076-1) - ADD - PL - -- - 634.3040601 - I61p - 1992000203 - DIS