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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
15/06/2016 |
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Data da última atualização: |
15/06/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MORAES, D. M. C.; DUARTE, S. C.; BASTOS, T. S. A.; REZENDE, C. L. G.; LEANDRO, N. S. M.; CAFÉ, M. B.; STRINGHINI, J. H.; ANDRADE, M. A. |
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Afiliação: |
DUNYA MARA CARDOSO MORAES, UFG/Escola de Veterinária e Zootecnia; SABRINA CASTILHO DUARTE, CNPSA; UFG/Escola de Veterinária e Zootecnia; UFG/Escola de Veterinária e Zootecnia; UFG/Escola de Veterinária e Zootecnia; UFG/Escola de Veterinária e Zootecnia; JOSÉ HENRIQUE STRINGHINI, UFG/Escola de Veterinária e Zootecnia; MARIA AUXILIADORA ANDRADE, UFG/Escola de Veterinária e Zootecnia. |
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Título: |
Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Brazilian Journal of Poultry Science, v. 18, n.1, p. 117-124, 2016. |
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DOI: |
http://dx.doi.org/10.1590/18069061-2015-006 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Conventional bacteriology techniques and quantitative polymerasechain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional bacteriology and 10.0% (9/90) by qPCR. In the 81 samples of unwashed brown eggs, Salmonella spp. was detected in 6.1% of the eggs (5/81) by conventional bacteriology and 27.2% (22/81) by qPCR. In the 102 samples of brown washed eggs, 6.9% (7/102) where positive by conventional bacteriology and 35.3% (16/102) by qPCR. All samples detected as positive by conventional bacteriology were also positive by qPCR. Salmonella Agona represented 18.2% (4/22) of identified serovars, Salmonella enterica subs. enterica O: 4.5 18.2% (4/22), Salmonella Schwarzengrund 18.2% (4/22), Salmonella Cerro 13.6% (3/22), Salmonella Anatum 13.6% (3/22), Salmonella Enteritidis 9.1% (2/22), Salmonella Johannesburg 4.5% (1/22), and Salmonella Corvallis 4.5% (1/22). The qPCR method provided better detection of Salmonella spp. in commercial eggs than conventional bacteriology. The conventional egg washing and disinfection procedures are not efficient to eliminate Salmonella. MenosAbstract: Conventional bacteriology techniques and quantitative polymerasechain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional bacteriology and 10.0% (9/90) by qPCR. In the 81 samples of unwashed brown eggs, Salmonella spp. was detected in 6.1% of the eggs (5/81) by conventional bacteriology and 27.2% (22/81) by qPCR. In the 102 samples of brown washed eggs, 6.9% (7/102) where positive by conventional bacteriology and 35.3% (16/102) by qPCR. All samples detected as positive by conventional bacteriology were also positive by qPCR. Salmonella Agona represented 18.2% (4/22) of identified serovars, Salmonella enterica subs. enterica O: 4.5 18.2% (4/22), Salmonella Schwarzengrund 18.2% (4/22), Salmonella Cerro 13.6% (3/22), Salmonella Anatum 13.6% (3/22), Salmonella Enteritidis 9.1% (2/22), Salmonella Johannesburg 4.5% (1/22), and Salmonella Corvallis 4.... Mostrar Tudo |
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Palavras-Chave: |
QPCR. |
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Thesagro: |
Bacteriologia; Casca de ovo; Gema de ovo; Salmonella. |
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Thesaurus Nal: |
Egg albumen; Egg shell; Egg yolk; Quantitative polymerase chain reaction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02730naa a2200325 a 4500
001 2047116
005 2016-06-15
008 2016 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/18069061-2015-006$2DOI
100 1 $aMORAES, D. M. C.
245 $aDetection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures.$h[electronic resource]
260 $c2016
520 $aAbstract: Conventional bacteriology techniques and quantitative polymerasechain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional bacteriology and 10.0% (9/90) by qPCR. In the 81 samples of unwashed brown eggs, Salmonella spp. was detected in 6.1% of the eggs (5/81) by conventional bacteriology and 27.2% (22/81) by qPCR. In the 102 samples of brown washed eggs, 6.9% (7/102) where positive by conventional bacteriology and 35.3% (16/102) by qPCR. All samples detected as positive by conventional bacteriology were also positive by qPCR. Salmonella Agona represented 18.2% (4/22) of identified serovars, Salmonella enterica subs. enterica O: 4.5 18.2% (4/22), Salmonella Schwarzengrund 18.2% (4/22), Salmonella Cerro 13.6% (3/22), Salmonella Anatum 13.6% (3/22), Salmonella Enteritidis 9.1% (2/22), Salmonella Johannesburg 4.5% (1/22), and Salmonella Corvallis 4.5% (1/22). The qPCR method provided better detection of Salmonella spp. in commercial eggs than conventional bacteriology. The conventional egg washing and disinfection procedures are not efficient to eliminate Salmonella.
650 $aEgg albumen
650 $aEgg shell
650 $aEgg yolk
650 $aQuantitative polymerase chain reaction
650 $aBacteriologia
650 $aCasca de ovo
650 $aGema de ovo
650 $aSalmonella
653 $aQPCR
700 1 $aDUARTE, S. C.
700 1 $aBASTOS, T. S. A.
700 1 $aREZENDE, C. L. G.
700 1 $aLEANDRO, N. S. M.
700 1 $aCAFÉ, M. B.
700 1 $aSTRINGHINI, J. H.
700 1 $aANDRADE, M. A.
773 $tBrazilian Journal of Poultry Science$gv. 18, n.1, p. 117-124, 2016.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 6 | |
| 2. |  | MORAES, D. M. C.; ANDRADE, M. A.; JAYME, V. de S.; STRINGHINI, J. H.; DUARTE, S. C. Resistance profile and virulence of isolates of salmonella sp. from commercial layer houses. In: EUROPEAN SYMPOSIUM ON THE QUALITY POULTRY MEAT, 22.; EUROPEAN SYMPOSIUM ON THE QUALITY OF EGGS AND EGG PRODUCTS, 16, 2015, Nantes. Abstract ...Nantes: [s.n.], 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 3. | | PEDROSO, A. A.; STRINGHINI, J. H.; LEANDRO, N. S. M.; CAFÉ, M. B.; BARBOSA, C. E.; LIMA, F. G. de. Suplementos utilizados como hidratantes nas fases pré-alojamento e pós-alojamento para pintos recém eclodidos. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 7, p. 627-632, jul. 2005 Título em inglês: Supplements used as hydrating agents in pre- and post-lodging phases to newly-hatched chicks.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | LEITE, P. R. de S. da C.; LEANDRO, N. S. M.; STRINGHINI, J. H.; CAFÉ, M. B.; GOMES, N. A.; JARDIM FILHO, R. de M. Desempenho de frangos de corte e digestibilidade de rações com sorgo ou milheto e complexo enzimático. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 3, p. 280-286, mar. 2011. Título em inglês: Broiler performance and digestibility of sorghum or millet diets with enzymatic complexes.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 6. | | MORAES, D. M. C.; DUARTE, S. C.; BASTOS, T. S. A.; REZENDE, C. L. G.; LEANDRO, N. S. M.; CAFÉ, M. B.; STRINGHINI, J. H.; ANDRADE, M. A. Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures. Brazilian Journal of Poultry Science, v. 18, n.1, p. 117-124, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 6 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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