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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
18/01/2023 |
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Data da última atualização: |
26/10/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TRICHEZ, D.; STEINDORFF, A. S.; MORAIS JÚNIOR, W. G. de; VILELA, N.; BERGMANN, J. C.; FORMIGHIERI, E. F.; GONCALVES, S. B.; ALMEIDA, J. R. M. de. |
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Afiliação: |
DÉBORA TRICHEZ, CNPAE; ANDREI S. STEINDORFF, CNPAE/ US DOE JOINT GENOME INSTITUTE; WILSON G. DE MORAIS JÚNIOR, CNPAE/ MILHOUSE INTERNATIONAL PTY; NATHÁLIA VILELA, CNPAE; JESSICA CARVALHO BERGMANN; EDUARDO FERNANDES FORMIGHIERI, CNPAE; SILVIA BELEM GONCALVES, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
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Título: |
Identification of traits to improve co-assimilation of glucose and xylose by adaptive evolution of Spathaspora passalidarum and Scheffersomyces stipitis yeasts. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, n. 107, p. 1143?1157, Feb. 2023. |
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DOI: |
https://doi.org/10.1007/s00253-023-12362-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Lignocellulosic biomass is a renewable raw material for producing several high-value-added chemicals and fuels. In general, xylose and glucose are the major sugars in biomass hydrolysates, and their efficient utilization by microorganisms is critical for an economical production process. Yeasts capable of co-consuming mixed sugars might lead to higher yields and productivities in industrial fermentation processes. Herein, we performed adaptive evolution assays with two xylose-fermenting yeasts, Spathaspora passalidarum and Scheffersomyces stipitis, to obtain derived clones with improved capabilities of glucose and xylose co-consumption. Adapted strains were obtained after successive growth selection using xylose and the non-metabolized glucose analog 2-deoxy-D-glucose as a selective pressure. The co-fermentation capacity of evolved and parental strains was evaluated on xylose-glucose mixtures. Our results revealed an improved co-assimilation capability by the evolved strains; however, xylose and glucose consumption were observed at slower rates than the parental yeasts. Genome resequencing of the evolved strains revealed genes affected by non-synonymous variants that might be involved with the co-consumption phenotype, including the HXT2.4 gene that encodes a putative glucose transporter in Sp. passalidarum. Expression of this mutant HXT2.4 in Saccharomyces cerevisiae improved the cells? co-assimilation of glucose and xylose. Therefore, our results demonstrated the successful improvement of co-fermentation through evolutionary engineering and the identification of potential targets for further genetic engineering of different yeast strains. MenosLignocellulosic biomass is a renewable raw material for producing several high-value-added chemicals and fuels. In general, xylose and glucose are the major sugars in biomass hydrolysates, and their efficient utilization by microorganisms is critical for an economical production process. Yeasts capable of co-consuming mixed sugars might lead to higher yields and productivities in industrial fermentation processes. Herein, we performed adaptive evolution assays with two xylose-fermenting yeasts, Spathaspora passalidarum and Scheffersomyces stipitis, to obtain derived clones with improved capabilities of glucose and xylose co-consumption. Adapted strains were obtained after successive growth selection using xylose and the non-metabolized glucose analog 2-deoxy-D-glucose as a selective pressure. The co-fermentation capacity of evolved and parental strains was evaluated on xylose-glucose mixtures. Our results revealed an improved co-assimilation capability by the evolved strains; however, xylose and glucose consumption were observed at slower rates than the parental yeasts. Genome resequencing of the evolved strains revealed genes affected by non-synonymous variants that might be involved with the co-consumption phenotype, including the HXT2.4 gene that encodes a putative glucose transporter in Sp. passalidarum. Expression of this mutant HXT2.4 in Saccharomyces cerevisiae improved the cells? co-assimilation of glucose and xylose. Therefore, our results demonstrated the successfu... Mostrar Tudo |
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Thesaurus Nal: |
Fermentation; Glucose; Sugar products. |
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Categoria do assunto: |
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Marc: |
LEADER 02532naa a2200253 a 4500
001 2151042
005 2023-10-26
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00253-023-12362-1$2DOI
100 1 $aTRICHEZ, D.
245 $aIdentification of traits to improve co-assimilation of glucose and xylose by adaptive evolution of Spathaspora passalidarum and Scheffersomyces stipitis yeasts.$h[electronic resource]
260 $c2023
520 $aLignocellulosic biomass is a renewable raw material for producing several high-value-added chemicals and fuels. In general, xylose and glucose are the major sugars in biomass hydrolysates, and their efficient utilization by microorganisms is critical for an economical production process. Yeasts capable of co-consuming mixed sugars might lead to higher yields and productivities in industrial fermentation processes. Herein, we performed adaptive evolution assays with two xylose-fermenting yeasts, Spathaspora passalidarum and Scheffersomyces stipitis, to obtain derived clones with improved capabilities of glucose and xylose co-consumption. Adapted strains were obtained after successive growth selection using xylose and the non-metabolized glucose analog 2-deoxy-D-glucose as a selective pressure. The co-fermentation capacity of evolved and parental strains was evaluated on xylose-glucose mixtures. Our results revealed an improved co-assimilation capability by the evolved strains; however, xylose and glucose consumption were observed at slower rates than the parental yeasts. Genome resequencing of the evolved strains revealed genes affected by non-synonymous variants that might be involved with the co-consumption phenotype, including the HXT2.4 gene that encodes a putative glucose transporter in Sp. passalidarum. Expression of this mutant HXT2.4 in Saccharomyces cerevisiae improved the cells? co-assimilation of glucose and xylose. Therefore, our results demonstrated the successful improvement of co-fermentation through evolutionary engineering and the identification of potential targets for further genetic engineering of different yeast strains.
650 $aFermentation
650 $aGlucose
650 $aSugar products
700 1 $aSTEINDORFF, A. S.
700 1 $aMORAIS JÚNIOR, W. G. de
700 1 $aVILELA, N.
700 1 $aBERGMANN, J. C.
700 1 $aFORMIGHIERI, E. F.
700 1 $aGONCALVES, S. B.
700 1 $aALMEIDA, J. R. M. de
773 $tApplied Microbiology and Biotechnology$gn. 107, p. 1143?1157, Feb. 2023.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
17/05/2002 |
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Data da última atualização: |
16/08/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BARROS, N. N.; VASCONCELOS, V. R. de; ZAPATA, J. F. F.; ARAUJO, M. R. de; MAGALHÃES, K. A. |
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Afiliação: |
NELSON NOGUEIRA BARROS, CNPC; VÂNIA RODRIGUES DE VASCONCELOS, CNPC; JORGE FERNANDO FUENTES ZAPATA, Universidade Federal do Ceará; MARCELO RENATO DE ARAÚJO, CNPC; KLÍNGER ARAGÃO MAGALHÃES, Graduação - Universidade Estadul Vale do Acaraú. |
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Título: |
Efeito do genótipo e da alimentação sobre características de carcaça de borrego para abate. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 38., 2001, Piracicaba. Anais... Piracicaba: FEALQ, 2001. p. 1390-1391. |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho objetivou determinar o rendimento de corte de carcaca de borregos submetidos a dietas de creep feeding (suplementacao durante a fase de amamentacao). |
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Palavras-Chave: |
Abattoir byproducts; Borrego; Caracteristicas de carcaca; Genotypes; Raça Santa Inês; Raça Somalis Brasileira. |
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Thesagro: |
Abate; Carcaça; Genótipo; Ovino; Rendimento; Suplemento alimentar. |
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Thesaurus NAL: |
Brazil; Carcass characteristics; Carcass composition; Creep feeding; Lambs; Sheep; Yields. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/48390/1/RAC-Efeito-do-genotipo.pdf
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Marc: |
LEADER 01315nam a2200385 a 4500
001 1528373
005 2021-08-16
008 2001 bl uuuu u00u1 u #d
100 1 $aBARROS, N. N.
245 $aEfeito do genótipo e da alimentação sobre características de carcaça de borrego para abate.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 38., 2001, Piracicaba. Anais... Piracicaba: FEALQ, 2001. p. 1390-1391.$c1391
520 $aEste trabalho objetivou determinar o rendimento de corte de carcaca de borregos submetidos a dietas de creep feeding (suplementacao durante a fase de amamentacao).
650 $aBrazil
650 $aCarcass characteristics
650 $aCarcass composition
650 $aCreep feeding
650 $aLambs
650 $aSheep
650 $aYields
650 $aAbate
650 $aCarcaça
650 $aGenótipo
650 $aOvino
650 $aRendimento
650 $aSuplemento alimentar
653 $aAbattoir byproducts
653 $aBorrego
653 $aCaracteristicas de carcaca
653 $aGenotypes
653 $aRaça Santa Inês
653 $aRaça Somalis Brasileira
700 1 $aVASCONCELOS, V. R. de
700 1 $aZAPATA, J. F. F.
700 1 $aARAUJO, M. R. de
700 1 $aMAGALHÃES, K. A.
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