Data/hora: 17/06/2024 23:51:48

Biblioteca(s): | Embrapa Amazônia Ocidental.
Data corrente: | 24/06/2015
Data da última atualização: | 03/03/2016
Autoria: | MEHRABI, R.; GOHARI, A. M.; SILVA, G. F. da; STEINBERG, G.; KEMA, G. H. J.; WIT, P. J. G. M. de.
Afiliação: | Rahim Mehrabi, Cereal Research Department; Amir Mirzadi Gohari, Plant Research International B.V.; GILVAN FERREIRA DA SILVA, CPAA; Gero Steinberg, University of Exeter; Gert H. J. Kema, Plant Research International B.V.; Pierre J. G. M. de Wit, Wageningen University.
Título: | Flexible gateway constructs for functional analyses of genes in plant pathogenic fungi.
Ano de publicação: | 2015
Fonte/Imprenta: | Fungal Genetics and Biology, v. 79, p. 186-192, 2015.
DOI: | http://dx.doi.org/10.1016/j.fgb.2015.03.016
Idioma: | Inglês
Conteúdo: | Genetic manipulation of fungi requires quick, low-cost, efficient, high-throughput and molecular tools. In this paper, we report 22 entry constructs as new molecular tools based on the Gateway technology facilitating rapid construction of binary vectors that can be used for functional analysis of genes in fungi. The entry vectors for single, double or triple gene-deletion mutants were developed using hygromycin, geneticin and nourseothricin resistance genes as selection markers. Furthermore, entry vectors containing green fluorescent (GFP) or red fluorescent (RFP) in combination with hygromycin, geneticin or nour- seothricin selection markers were generated. The latter vectors provide the possibility of gene deletion and simultaneous labelling of the fungal transformants with GFP or RFP reporter genes. The applicability of a number of entry vectors was validated in Zymoseptoria tritici, an important fungal wheat pathogen
Palavras-Chave: | Gateway technology; Zymoseptoria tritici.
Thesaurus Nal: | gene deletion.
Categoria do assunto: | --
Registro original: | Embrapa Amazônia Ocidental (CPAA)
Exemplares: | CPAA - (30123-1) - UPC - AP - DD -  -  -  - DIS