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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
10/06/1999 |
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Data da última atualização: |
10/06/1999 |
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Autoria: |
PEDROSA, F. O.; TEIXEIRA, K. R. dos S.; MACHADO, I. M. P.; STEFFENS, M. B. R.; KLASSEN, G.; BENELLI, E. M.; MACHADO, H. B.; FUNAYAMA, S.; RIGO, L. U.; ISHIDA, M. L.; YATES, M. G.; SOUZA, E. M. |
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Título: |
Structural organization and regulation of the Nif genes of Herbaspirillum seropedicae. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Soil Biology and Biochemistry, Oxford, v. 29, n. 5/6, p. 843-846, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
The promoter regions of nifA and nifH genes of Herbaspirillum seropedicae have been isolated and investigated, and the nifA promoter has been characterized in detail. Both regions contain a -24/-12 type promoter and nifA-binding sites; the nifA promoter also has an NtrC-binding site. The nifA expression is activated by NtrC and repressed by ammonium ions but not by oxygen. When the NtrC-binding site was deleted nifA expression was NifA-dependent. The native NifA protein of H. seropedicae was unable to activate nifH expression in Escherichia coli whereas a truncated protein, lacking the N-terminal domain was able to activate nifH expression in the absence of oxygen and presence of iron, indicating that the N-terminal domain regulates NifA activity. The truncated protein also activates nifH in Azospirillum brasilense in the presence or absence of ammonium ions. This suggests that the N-terminal domain senses the nitrogen status of the cell. |
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Palavras-Chave: |
BNF; FBN; Fixação biologica de nitrogênio; Nitrogen fixing bacteria. |
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Thesagro: |
Gene; Proteína. |
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Thesaurus Nal: |
genes; proteins. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01903naa a2200349 a 4500
001 1621090
005 1999-06-10
008 1997 bl uuuu u00u1 u #d
100 1 $aPEDROSA, F. O.
245 $aStructural organization and regulation of the Nif genes of Herbaspirillum seropedicae.
260 $c1997
520 $aThe promoter regions of nifA and nifH genes of Herbaspirillum seropedicae have been isolated and investigated, and the nifA promoter has been characterized in detail. Both regions contain a -24/-12 type promoter and nifA-binding sites; the nifA promoter also has an NtrC-binding site. The nifA expression is activated by NtrC and repressed by ammonium ions but not by oxygen. When the NtrC-binding site was deleted nifA expression was NifA-dependent. The native NifA protein of H. seropedicae was unable to activate nifH expression in Escherichia coli whereas a truncated protein, lacking the N-terminal domain was able to activate nifH expression in the absence of oxygen and presence of iron, indicating that the N-terminal domain regulates NifA activity. The truncated protein also activates nifH in Azospirillum brasilense in the presence or absence of ammonium ions. This suggests that the N-terminal domain senses the nitrogen status of the cell.
650 $agenes
650 $aproteins
650 $aGene
650 $aProteína
653 $aBNF
653 $aFBN
653 $aFixação biologica de nitrogênio
653 $aNitrogen fixing bacteria
700 1 $aTEIXEIRA, K. R. dos S.
700 1 $aMACHADO, I. M. P.
700 1 $aSTEFFENS, M. B. R.
700 1 $aKLASSEN, G.
700 1 $aBENELLI, E. M.
700 1 $aMACHADO, H. B.
700 1 $aFUNAYAMA, S.
700 1 $aRIGO, L. U.
700 1 $aISHIDA, M. L.
700 1 $aYATES, M. G.
700 1 $aSOUZA, E. M.
773 $tSoil Biology and Biochemistry, Oxford$gv. 29, n. 5/6, p. 843-846, 1997.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| 1. |  | OLIVEIRA, M. do S. P. de; FERREIRA, S. F.; OLIVIERA, N. P. de; OLIVEIRA, E. M. de. Similaridade genética entre açaizeiros (Euterpe oleracea Mart.) procedentes de Breves, Pará, Brasil baseada em marcadores RAPD. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, 7., 2009, Pucón, Chile. Proceeding... Santiago de Chile: Ministério de Agricultura, Instituto de Investigaciones Agropecuarias, 2009. p. 277-278.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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