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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
08/02/2019 |
Data da última atualização: |
11/02/2019 |
Tipo da produção científica: |
Nota Técnica/Nota Científica |
Autoria: |
SANTOS, J. D. R.; ARASHIRO, E. K. N.; BALARO, M. F. A.; SOUZA-FABJAN, J. M. G.; PINTO, N. H. N.; SOUZA, C. V. de; LEITE, C. R.; FONSECA, J. F. da; BRANDÃO, F. Z. |
Afiliação: |
JULIANA DANTAS RODRIGUES SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; EDUARDO KENJI NUNES ARASHIRO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; MÁRIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; PEDRO HENRIQUE NICOLAU PINTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; CLARA VIEIRA DE SOUZA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; CECI RIBEIRO LEITE, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
Título: |
Cervical transposition test using Hegar dilator at oestrus as a tool to select ewes for transcervical embryo collection. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 54, n. 1, p. 126-128, Sept. 2019. |
DOI: |
10.1111/rda.13306 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study evaluated the cervical transposition method as a tool to select ewes for embryo collection by transcervical route. Adult Santa Inês ewes (n = 50) received Day 0 protocol for superovulation treatments. The cervix transposition test was performed both at oestrus and at the embryo collection time. The latter was preceded by hormonal cervical dilation. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 85.7%, 66.6%, 85.7%, 66.6% and 80.0%, respectively. The kappa index yielded a moderate score (? = 0.52). In conclusion, the high sensitivity and accuracy indicate that the cervical transposition test is a screening option to select ewes for embryo collection by transcervical route. |
Palavras-Chave: |
Cervical dilation; MOET; Raça Santa Inês. |
Thesagro: |
Eficiência Reprodutiva; Ovelha; Ovino; Reprodução Animal; Superovulação; Transferência de Embrião. |
Thesaurus Nal: |
Cervix; Embryo transfer; Ewes; Reproduction; Reproductive performance; Screening; Sheep; Superovulation. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02011naa a2200433 a 4500 001 2105729 005 2019-02-11 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1111/rda.13306$2DOI 100 1 $aSANTOS, J. D. R. 245 $aCervical transposition test using Hegar dilator at oestrus as a tool to select ewes for transcervical embryo collection.$h[electronic resource] 260 $c2019 520 $aAbstract: This study evaluated the cervical transposition method as a tool to select ewes for embryo collection by transcervical route. Adult Santa Inês ewes (n = 50) received Day 0 protocol for superovulation treatments. The cervix transposition test was performed both at oestrus and at the embryo collection time. The latter was preceded by hormonal cervical dilation. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 85.7%, 66.6%, 85.7%, 66.6% and 80.0%, respectively. The kappa index yielded a moderate score (? = 0.52). In conclusion, the high sensitivity and accuracy indicate that the cervical transposition test is a screening option to select ewes for embryo collection by transcervical route. 650 $aCervix 650 $aEmbryo transfer 650 $aEwes 650 $aReproduction 650 $aReproductive performance 650 $aScreening 650 $aSheep 650 $aSuperovulation 650 $aEficiência Reprodutiva 650 $aOvelha 650 $aOvino 650 $aReprodução Animal 650 $aSuperovulação 650 $aTransferência de Embrião 653 $aCervical dilation 653 $aMOET 653 $aRaça Santa Inês 700 1 $aARASHIRO, E. K. N. 700 1 $aBALARO, M. F. A. 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aPINTO, N. H. N. 700 1 $aSOUZA, C. V. de 700 1 $aLEITE, C. R. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDÃO, F. Z. 773 $tReproduction in Domestic Animals$gv. 54, n. 1, p. 126-128, Sept. 2019.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
21/11/2012 |
Data da última atualização: |
14/04/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 5 |
Autoria: |
D'AFONSECA, V.; SOARES, S. C.; ALI, A.; SANTOS, A. R.; PINTO, A. C.; MAGALHÃES, A. A. C.; FARIA, C. de J.; BARBOSA, E.; GUIMARÃES, L. C.; ESLABÃO, M.; ALMEIDA, S. S.; ABREU, V. A. C.; ZERLOTINI, A.; CARNEIRO, A. R.; CERDEIRA, L. T.; RAMOS, R. T. J.; HIRATA JÚNIOR, R.; MATTOS-GUARALDI, A. L.; TROST, E.; TAUCH, A.; SILVA, A.; SCHNEIDER, M. P.; MIYOSHI, A.; AZEVEDO, V. |
Afiliação: |
VÍVIAN D’AFONSECA, UFMG; SIOMAR C. SOARES, UFMG; AMJAD ALI, UFMG; ANDERSON R. SANTOS, UFMG; ANNE C. PINTO, UFMG; ARYANE A. C. MAGALHÃES, UFMG; CÁSSIO DE JESUS FARIA, UFMG; EUDES BARBOSA, UFMG; LUIS C. GUIMARÃES, UFMG; MARCUS ESLABÃO, UFPel; SINTIA S. ALMEIDA, UFMG; VINICIUS A. C. ABREU, UFMG; ADHEMAR ZERLOTINI NETO, CNPTIA; ADRIANA R. CARNEIRO, UFPA; LOUISE T. CERDEIRA, UFPA; ROMMEL T. J. RAMOS, UFPA; RAPHAEL HIRATA JÚNIOR, UERJ; ANA L. MATTOS-GUARALDI, UERJ; EVA TROST, Bielefeld University; ANDREAS TAUCH, Bielefeld University; ARTUR SILVA, UFPA; MARIA P. SCHNEIDER, UFPA; ANDERSON MIYOSHI, UFMG; VASCO AZEVEDO, UFMG. |
Título: |
Reannotation of the Corynebacterium diphtheriae NCTC13129 genome as a new approach to studying gene targets connected to virulence and pathogenicity in diphtheria. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Open Access Bioinformatics, v. 4, p. 1-13, 2012. |
Idioma: |
Inglês |
Conteúdo: |
Background: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already described in the literature. In addition, the large number of transposases and the presence of structural genes of bacteriophages make their genomic versatility evident. Contrasting with this reality, it also allowed the clarification of some aspects concerned with mechanisms used by C. diphtheriae to stop the invasion of the genome by bacteriophages, mediated by the clustered regularly interspaced short palindromic repeats region. Conclusion: The reannotation of the C. diphtheriae genome provided an improvement in annotation of the C. diphtheriae genome in several aspects, such as virulence characteristics and plasticity events. Moreover, the protocol used here can be extended to various other pathogens in order to improve the genomic information already on file in public databases and to minimize propagating errors. The reannotated archive and updated archive are available at: http://lgcm.icb.ufmg.br/pub/C_diphtheriae_reannotation.embl. MenosBackground: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already des... Mostrar Tudo |
Palavras-Chave: |
Bioinformática. |
Thesagro: |
Genoma; Patogenicidade. |
Thesaurus NAL: |
Bioinformatics; Corynebacterium diphtheriae; Genome; Pathogenicity islands. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/70533/1/OAB-25500-re-annotation-of-the-corynebacterium-diphtheriae-nctc13129-g-022412.pdf
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Marc: |
LEADER 03832naa a2200481 a 4500 001 1940203 005 2020-04-14 008 2012 bl uuuu u00u1 u #d 100 1 $aD'AFONSECA, V. 245 $aReannotation of the Corynebacterium diphtheriae NCTC13129 genome as a new approach to studying gene targets connected to virulence and pathogenicity in diphtheria.$h[electronic resource] 260 $c2012 520 $aBackground: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already described in the literature. In addition, the large number of transposases and the presence of structural genes of bacteriophages make their genomic versatility evident. Contrasting with this reality, it also allowed the clarification of some aspects concerned with mechanisms used by C. diphtheriae to stop the invasion of the genome by bacteriophages, mediated by the clustered regularly interspaced short palindromic repeats region. Conclusion: The reannotation of the C. diphtheriae genome provided an improvement in annotation of the C. diphtheriae genome in several aspects, such as virulence characteristics and plasticity events. Moreover, the protocol used here can be extended to various other pathogens in order to improve the genomic information already on file in public databases and to minimize propagating errors. The reannotated archive and updated archive are available at: http://lgcm.icb.ufmg.br/pub/C_diphtheriae_reannotation.embl. 650 $aBioinformatics 650 $aCorynebacterium diphtheriae 650 $aGenome 650 $aPathogenicity islands 650 $aGenoma 650 $aPatogenicidade 653 $aBioinformática 700 1 $aSOARES, S. C. 700 1 $aALI, A. 700 1 $aSANTOS, A. R. 700 1 $aPINTO, A. C. 700 1 $aMAGALHÃES, A. A. C. 700 1 $aFARIA, C. de J. 700 1 $aBARBOSA, E. 700 1 $aGUIMARÃES, L. C. 700 1 $aESLABÃO, M. 700 1 $aALMEIDA, S. S. 700 1 $aABREU, V. A. C. 700 1 $aZERLOTINI, A. 700 1 $aCARNEIRO, A. R. 700 1 $aCERDEIRA, L. T. 700 1 $aRAMOS, R. T. J. 700 1 $aHIRATA JÚNIOR, R. 700 1 $aMATTOS-GUARALDI, A. L. 700 1 $aTROST, E. 700 1 $aTAUCH, A. 700 1 $aSILVA, A. 700 1 $aSCHNEIDER, M. P. 700 1 $aMIYOSHI, A. 700 1 $aAZEVEDO, V. 773 $tOpen Access Bioinformatics$gv. 4, p. 1-13, 2012.
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