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Registro Completo |
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
29/12/2015 |
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Data da última atualização: |
29/12/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LIMA, C. C. F. de; FUKUSHIMA, G. S.; SANCHEZ, P. E. S.; IEYASU, A. C. F.; SOUZA, V. B. de; SOUZA, F. B. de; SILVA, W. M. |
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Afiliação: |
CARINE CAVALCANTI FARIA DE LIMA, Graduanda em Biomedicina - Universidade da Grande Dourados, Dourados, MS; GEISIENE SANT’ANNA FUKUSHIMA, Graduanda em Agronomia - Faculdade Anhanguera de Dourados, Dourados, MS.; PABLO EDUARDO SANTOS SANCHEZ, Graduando em Técnico em Química – SENAI, Dourados, MS; ANA CLÁUDIA FELIX IEYASU, Graduanda em Técnico em Química – SENAI, Dourados, MS; VINICIUS BETONI DE SOUZA, Graduando em Técnico Agroindústria - SENAI, Dourados, MS.; FERNANDA BARBOZA DE SOUZA, Graduanda em Química - Universidade Estadual de Mato Grosso do Sul, Dourados, MS; WILLIAM MARRA SILVA, CPAO. |
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Título: |
Comparação entre dois métodos analíticos para determinação de nitrogênio em tecido vegetal. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO À PESQUISA DA EMBRAPA, 2015, Dourados. Resumos... Brasília, DF: Embrapa, 2015. JIPE 2015. |
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Idioma: |
Português |
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Palavras-Chave: |
Método de dumas; Método de Kjeldahl; Nitrogênio total; Química limpa. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136253/1/8-Carine-comparacao.pdf
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Marc: |
LEADER 00762nam a2200217 a 4500
001 2032538
005 2015-12-29
008 2015 bl uuuu u00u1 u #d
100 1 $aLIMA, C. C. F. de
245 $aComparação entre dois métodos analíticos para determinação de nitrogênio em tecido vegetal.$h[electronic resource]
260 $aIn: JORNADA DE INICIAÇÃO À PESQUISA DA EMBRAPA, 2015, Dourados. Resumos... Brasília, DF: Embrapa, 2015. JIPE 2015.$c2015
653 $aMétodo de dumas
653 $aMétodo de Kjeldahl
653 $aNitrogênio total
653 $aQuímica limpa
700 1 $aFUKUSHIMA, G. S.
700 1 $aSANCHEZ, P. E. S.
700 1 $aIEYASU, A. C. F.
700 1 $aSOUZA, V. B. de
700 1 $aSOUZA, F. B. de
700 1 $aSILVA, W. M.
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Registro original: |
Embrapa Agropecuária Oeste (CPAO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Café; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/02/2011 |
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Data da última atualização: |
28/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
VIDAL, R. O.; MONDEGO, J. M. C.; POT, D.; AMBRÓSIO, A. B.; ANDRADE, A. C.; PEREIRA, L. F. P.; COLOMBO, C. A.; VIEIRA, L. G. E.; CARAZZOLLE, M. F.; PEREIRA, G. A. G. |
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Afiliação: |
RAMON OLIVEIRA VIDAL, UNICAMP/Instituto de Biologia; JORGE MAURÍCIO COSTA MONDEGO, Instituto Agronômico de Campinas; DAVID POT, Centre de Coopération Internationale en Recherche Agronomique pour le Développement; ALINNE BATISTA AMBRÓSIO, UNICAMP/Instituto de Biologia; ALAN CARVALHO ANDRADE, CENARGEN; LUIZ FILIPE PROTASIO PEREIRA, SAPC; CARLOS AUGUSTO COLOMBO, Instituto Agronômico de Campinas; LUIZ GONZAGA ESTEVES VIEIRA, Instituo Agronômico do Pará; MARCELO FALSARELLA CARAZZOLLE, UNICAMP/Instituto de Biologia; GONÇALO AMARANTE GUIMARÃES PEREIRA, UNICAMP/Instituto de Biologia. |
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Título: |
A high-throughput data mining of single nucleotide polymorphisms in Coffea species expresed sequence tags suggests differential homeologous gene expression in the allotetrapoloid Coffea arabica. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
PLANT PHYSIOLOGY, v. 154, p. 1053-1066. 2010. |
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Páginas: |
1053-1066 |
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Idioma: |
Inglês |
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Conteúdo: |
Polyploidization constitutes a common mode of evolution in flowering plants. This event provides the raw material for the divergence of function in homeologous genes, leading to phenotypic novelty that can contribute to the success of polyploids in nature or their selection for use in agriculture. Mounting evidence underlined the existence of homeologous expression biases in polyploid genomes; however, strategies to analyze such transcriptome regulation remained scarce. Important factors regarding homeologous expression biases remain to be explored, such as whether this phenomenon influences specific genes, how paralogs are affected by genome doubling, and what is the importance of the variability of homeologous expression bias to genotype differences. This study reports the expressed sequence tag assembly of the allopolyploid Coffea arabica and one of its direct ancestors, Coffea canephora. The assembly was used for the discovery of single nucleotide polymorphisms through the identification of high-quality discrepancies in overlapped expressed sequence tags and for gene expression information indirectly estimated by the transcript redundancy. Sequence diversity profiles were evaluated within C. arabica (Ca) and C. canephora (Cc) and used to deduce the transcript contribution of the Coffea eugenioides (Ce) ancestor. The assignment of the C. arabica haplotypes to the C. canephora (CaCc) or C. eugenioides (CaCe) ancestral genomes allowed us to analyze gene expression contributions of each subgenome in C. arabica. In silico data were validated by the quantitative polymerase chain reaction and allele-specific combination TaqMAMA-based method. The presence of differential expression of C. arabica homeologous genes and its implications in coffee gene expression, ontology, and physiology are discussed. MenosPolyploidization constitutes a common mode of evolution in flowering plants. This event provides the raw material for the divergence of function in homeologous genes, leading to phenotypic novelty that can contribute to the success of polyploids in nature or their selection for use in agriculture. Mounting evidence underlined the existence of homeologous expression biases in polyploid genomes; however, strategies to analyze such transcriptome regulation remained scarce. Important factors regarding homeologous expression biases remain to be explored, such as whether this phenomenon influences specific genes, how paralogs are affected by genome doubling, and what is the importance of the variability of homeologous expression bias to genotype differences. This study reports the expressed sequence tag assembly of the allopolyploid Coffea arabica and one of its direct ancestors, Coffea canephora. The assembly was used for the discovery of single nucleotide polymorphisms through the identification of high-quality discrepancies in overlapped expressed sequence tags and for gene expression information indirectly estimated by the transcript redundancy. Sequence diversity profiles were evaluated within C. arabica (Ca) and C. canephora (Cc) and used to deduce the transcript contribution of the Coffea eugenioides (Ce) ancestor. The assignment of the C. arabica haplotypes to the C. canephora (CaCc) or C. eugenioides (CaCe) ancestral genomes allowed us to analyze gene expression contribut... Mostrar Tudo |
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Thesagro: |
Coffea Arábica. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29342/1/A-high-throughput.pdf
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Marc: |
LEADER 02657naa a2200253 a 4500
001 1917245
005 2023-02-28
008 2010 bl uuuu u00u1 u #d
100 1 $aVIDAL, R. O.
245 $aA high-throughput data mining of single nucleotide polymorphisms in Coffea species expresed sequence tags suggests differential homeologous gene expression in the allotetrapoloid Coffea arabica.$h[electronic resource]
260 $c2010
300 $a1053-1066
520 $aPolyploidization constitutes a common mode of evolution in flowering plants. This event provides the raw material for the divergence of function in homeologous genes, leading to phenotypic novelty that can contribute to the success of polyploids in nature or their selection for use in agriculture. Mounting evidence underlined the existence of homeologous expression biases in polyploid genomes; however, strategies to analyze such transcriptome regulation remained scarce. Important factors regarding homeologous expression biases remain to be explored, such as whether this phenomenon influences specific genes, how paralogs are affected by genome doubling, and what is the importance of the variability of homeologous expression bias to genotype differences. This study reports the expressed sequence tag assembly of the allopolyploid Coffea arabica and one of its direct ancestors, Coffea canephora. The assembly was used for the discovery of single nucleotide polymorphisms through the identification of high-quality discrepancies in overlapped expressed sequence tags and for gene expression information indirectly estimated by the transcript redundancy. Sequence diversity profiles were evaluated within C. arabica (Ca) and C. canephora (Cc) and used to deduce the transcript contribution of the Coffea eugenioides (Ce) ancestor. The assignment of the C. arabica haplotypes to the C. canephora (CaCc) or C. eugenioides (CaCe) ancestral genomes allowed us to analyze gene expression contributions of each subgenome in C. arabica. In silico data were validated by the quantitative polymerase chain reaction and allele-specific combination TaqMAMA-based method. The presence of differential expression of C. arabica homeologous genes and its implications in coffee gene expression, ontology, and physiology are discussed.
650 $aCoffea Arábica
700 1 $aMONDEGO, J. M. C.
700 1 $aPOT, D.
700 1 $aAMBRÓSIO, A. B.
700 1 $aANDRADE, A. C.
700 1 $aPEREIRA, L. F. P.
700 1 $aCOLOMBO, C. A.
700 1 $aVIEIRA, L. G. E.
700 1 $aCARAZZOLLE, M. F.
700 1 $aPEREIRA, G. A. G.
773 $tPLANT PHYSIOLOGY$gv. 154, p. 1053-1066. 2010.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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