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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
16/06/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
CASTRO, B. G.; SOUZA, M. M. S.; REGUA-MANGIA, A. H.; BITTENCOURT, A. J. |
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Afiliação: |
BRUNO G. CASTRO, UFMT; MILIANE M. S. SOUZA, UFRRJ; ADRIANA H. REGUA-MANGIA, FIOCRUZ; AVELINO J. BITTENCOURT, UFRRJ. |
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Título: |
Genetic relationship between Escherichia coli strains isolated from dairy mastitis and from the stable fly Stomoxys calcitrans. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 6, p. 479-484, junho. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
The stable fly Stomoxys calcitrans (Linnaeus, 1758) has been described as a potential spreader of infectious agents to cattle herds. Among the agents transmitted by this fly, Escherichia coli has attracted attention due to its potential to cause gastrointestinal disorders as well as environmental mastitis in dairy cows. Therefore, the aim of this study was to isolate and to assess the genetic diversity and the clonal relatedness among E. coli isolates from the milk of dairy mastitis and from stable flies anatomical sites by the Random Amplification of Polymorphic DNA (RAPD-PCR) technique. The molecular typing revealed a high degree of genetic polymorphism suggesting that these microorganisms have a non-clonal origin. Identical electrophoretic profiles were observed between E. coli isolates from different flies, different mammary quarters of the same cow and from cows on a single farm. These results reveal the circulation of the same bacterial lineages and suggest the role of the stable fly in bacterial dispersion. Considering the high pathogenic potential of this bacterial species, our findings alert to a more effective health surveillance. |
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Palavras-Chave: |
Amplificação rondômica do DNA; Clonal species; Moscas dos estábulos; Random amplification of polymorphic DNA; RAPDA; Stable fly. |
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Thesagro: |
Clone; Stomoxys Calcitrans. |
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Thesaurus Nal: |
Milk. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/144465/1/Genetic-relationship.pdf
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Marc: |
LEADER 02001naa a2200265 a 4500
001 2047177
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aCASTRO, B. G.
245 $aGenetic relationship between Escherichia coli strains isolated from dairy mastitis and from the stable fly Stomoxys calcitrans.
260 $c2016
520 $aThe stable fly Stomoxys calcitrans (Linnaeus, 1758) has been described as a potential spreader of infectious agents to cattle herds. Among the agents transmitted by this fly, Escherichia coli has attracted attention due to its potential to cause gastrointestinal disorders as well as environmental mastitis in dairy cows. Therefore, the aim of this study was to isolate and to assess the genetic diversity and the clonal relatedness among E. coli isolates from the milk of dairy mastitis and from stable flies anatomical sites by the Random Amplification of Polymorphic DNA (RAPD-PCR) technique. The molecular typing revealed a high degree of genetic polymorphism suggesting that these microorganisms have a non-clonal origin. Identical electrophoretic profiles were observed between E. coli isolates from different flies, different mammary quarters of the same cow and from cows on a single farm. These results reveal the circulation of the same bacterial lineages and suggest the role of the stable fly in bacterial dispersion. Considering the high pathogenic potential of this bacterial species, our findings alert to a more effective health surveillance.
650 $aMilk
650 $aClone
650 $aStomoxys Calcitrans
653 $aAmplificação rondômica do DNA
653 $aClonal species
653 $aMoscas dos estábulos
653 $aRandom amplification of polymorphic DNA
653 $aRAPDA
653 $aStable fly
700 1 $aSOUZA, M. M. S.
700 1 $aREGUA-MANGIA, A. H.
700 1 $aBITTENCOURT, A. J.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 6, p. 479-484, junho. 2016.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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| Registros recuperados : 5 | |
| 2. |  | BRONZATO, G. F.; OLIVA, M. S.; ALVIN, M. G.; PRIBUL, B. R.; RODRIGUES, D. P.; COELHO, S. M. O.; COELHO, I. S.; SOUZA, M. M. S. MALDI-TOF MS as a tool for the identification of Vibrio alginolyticus from Perna perna mussels (Linnaeus, 1758). Pesquisa Veterinária Brasileira, Brasília, DF, v. 38, n 8, p. 1511-1517, agosto 2018 Título em português: MALDI-TOF MS como ferramenta na identificação de Vibrio alginolyticus isolados de mexilhões Perna perna (Linnaeus, 1758).| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | FERREIRA, P. F. A.; XAVIER, J. F.; BERTHOLOTO, D. M.; MELO, D. A. de; CORREIA, T. R.; COELHO, S. de M. de O.; SOUZA, M. M. S. de; LEAL, M. A. de A.; ARAUJO, E. da S. Effect of composting on the microbiological and parasitic load in animal production wastes in Brazil. International Journal of Recycling of Organic Waste in Agriculture, v. 10, n. 3, p. 265-373-, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 4. | | MONTEIRO, H. H. M. S.; OLIVEIRA, C. C. M. S.; COELHO, S. M. O.; SOUZA, M. M. S.; NETO, A. M. L.; RIBEIRO, B. S.; PIRES, P. P.; SANAVRIA, A. Microbiota bacteriana presente em lesões causadas pelas larvas de Cochliomyia hominivorax coquerel, 1858 (Calliphoridae) em bezerros naturalmente infestados da Embrapa - MS (CNPGC). Revista Brasileira de Parasitologia Veterinária, São Paulo, v.13, p. 342, 2004. Suplemento 1, ref. 530. Edição anais do XIII Congresso Brasileiro de Parasitologia Veterinária e I Simpósio Latino-Americano de Rickettsioses, Ouro Preto, MG, set. 2004. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 5. | | FERREIRA, P. F. A.; XAVIER, J. F.; BERTHOLOTO, D. M.; MELO, D. A. de; CORREIRA, T. R.; COELHO, S. de MATTOS de O.; SOUZA, M. M. S. de; LEAL, M. A. de A.; ARAUJO, E. da S.; COELHO, I. da S. Effect of composting on the microbiological and parasitic load in animal production wastes in Brazil. International Journal of Recycling of Organic Waste, v. 10, n.3, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 5 | |
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