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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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Data corrente: |
09/07/2003 |
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Data da última atualização: |
11/07/2005 |
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Autoria: |
SIHLER, W.; OLIVEIRA, M. S.; CARNEIRO, M.; SOUZA, M. L. de. |
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Título: |
Expressao do gene rolA em sistema Baculovirus. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Brasilia: Embrapa Recursos Geneticos e Biotecnologia, 2002. |
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Páginas: |
26 p. |
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Série: |
(Embrapa Recursos Geneticos e Biotecnologia. Boletim de Pesquisa e Desenvolvimento, 38).
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Idioma: |
Português |
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Palavras-Chave: |
gene rolA; Sistema baculovirus; Sistema de expressao. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CENARGEN/23673/1/bp038.pdf
https://www.cenargen.embrapa.br/publica/trabalhos/bp038.pdf
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Marc: |
LEADER 00606nam a2200193 a 4500
001 1184685
005 2005-07-11
008 2002 bl uuuu u0uu1 u #d
100 1 $aSIHLER, W.
245 $aExpressao do gene rolA em sistema Baculovirus.
260 $aBrasilia: Embrapa Recursos Geneticos e Biotecnologia$c2002
300 $a26 p.
490 $a(Embrapa Recursos Geneticos e Biotecnologia. Boletim de Pesquisa e Desenvolvimento, 38).
653 $agene rolA
653 $aSistema baculovirus
653 $aSistema de expressao
700 1 $aOLIVEIRA, M. S.
700 1 $aCARNEIRO, M.
700 1 $aSOUZA, M. L. de
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
26/07/2012 |
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Data da última atualização: |
22/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SUDANO, M. J.; PASCHOAL, D. M.; RASCADO, T. da S.; CROCOMO, L. F.; MAGALHÃES, L. C. O.; MARTINS JÚNIOR, A.; MACHADO, R.; LANDIM-ALVARENGA, F. da C. |
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Afiliação: |
MATEUS JOSÉ SUDANO, POS GRADUAÇÃO UNESP/BOTUCATU; DANIELA MARTINS PASCHOAL, POS GRADUAÇÃO UNESP/BOTUCATU; TATIANA DA SILVA RASCADO, POS GRADUAÇÃO UNESP/BOTUCATU; LETICIA FERRARI CROCOMO, POS GRADUAÇÃO UNESP/BOTUCATU; LUIZ CARLOS O. MAGALHÃES, POS GRADUAÇÃO UNESP/BOTUCATU; ALÍCIO MARTINS JÚNIOR, POS GRADUAÇÃO UNESP/ARAÇATUBA; RUI MACHADO, CPPSE; FERNANDO DA CRUZ LANDIM ALVARENGA, POS GRADUAÇÃO UNESP/BOTUCATU. |
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Título: |
Crucial surviving aspects for vitrified in vitro-produced bovine embryos. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Zygote, v. 20, p. 1-8, 2012. |
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DOI: |
10.1017/S0967199412000196 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of the present study was to correlate some parameters (cleavage, blastocyst production, quality degree score, total cell number, fresh apoptosis and lipid content) with embryo survival after cryopreservation. A total of 1727 in vitro-produced bovine blastocysts were used to establish the parameters (mean ± standard error of the mean (SEM)) for cleavage (85.6 ± 0.8), blastocyst production (39.9 ± 1.4), quality degree score (1.6 ± 0.1), total cell number (140.1 ± 2.9), fresh apoptosis (20.8 ± 1.1) and lipid content (21.3 ± 0.8 droplets). On the same way 1316 blastocysts were vitrified for the determination of post-cryopreservation embryo survival (49.4 ± 1.9). Fresh apoptosis rate and total lipid droplets value were correlated (P < 0.05) with embryo survival after cryopreservation (r = 0.91 and r = 0.59; respectively). However, cleavage, blastocyst production, quality degree score and total cell number were not correlated (P > 0.05) with embryo cryotolerance (r = 0.23, r = 0.38, r = 0.22 and r = 0.28; respectively). Therefore, the increased lipid content was moderately correlated with apoptosis in vitrified blastocysts. On the other hand, increased apoptosis in fresh blastocysts was strongly correlated with apoptosis in vitrified blastocysts, which indicated that the apoptosis rate in fresh embryos was a better parameter than the lipid content to predict post-vitrification embryo survival. |
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Palavras-Chave: |
Cryotolerance; Embryo quality. |
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Thesaurus NAL: |
apoptosis; lipid content. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02189naa a2200265 a 4500
001 1929526
005 2023-02-22
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1017/S0967199412000196$2DOI
100 1 $aSUDANO, M. J.
245 $aCrucial surviving aspects for vitrified in vitro-produced bovine embryos.$h[electronic resource]
260 $c2012
520 $aThe objective of the present study was to correlate some parameters (cleavage, blastocyst production, quality degree score, total cell number, fresh apoptosis and lipid content) with embryo survival after cryopreservation. A total of 1727 in vitro-produced bovine blastocysts were used to establish the parameters (mean ± standard error of the mean (SEM)) for cleavage (85.6 ± 0.8), blastocyst production (39.9 ± 1.4), quality degree score (1.6 ± 0.1), total cell number (140.1 ± 2.9), fresh apoptosis (20.8 ± 1.1) and lipid content (21.3 ± 0.8 droplets). On the same way 1316 blastocysts were vitrified for the determination of post-cryopreservation embryo survival (49.4 ± 1.9). Fresh apoptosis rate and total lipid droplets value were correlated (P < 0.05) with embryo survival after cryopreservation (r = 0.91 and r = 0.59; respectively). However, cleavage, blastocyst production, quality degree score and total cell number were not correlated (P > 0.05) with embryo cryotolerance (r = 0.23, r = 0.38, r = 0.22 and r = 0.28; respectively). Therefore, the increased lipid content was moderately correlated with apoptosis in vitrified blastocysts. On the other hand, increased apoptosis in fresh blastocysts was strongly correlated with apoptosis in vitrified blastocysts, which indicated that the apoptosis rate in fresh embryos was a better parameter than the lipid content to predict post-vitrification embryo survival.
650 $aapoptosis
650 $alipid content
653 $aCryotolerance
653 $aEmbryo quality
700 1 $aPASCHOAL, D. M.
700 1 $aRASCADO, T. da S.
700 1 $aCROCOMO, L. F.
700 1 $aMAGALHÃES, L. C. O.
700 1 $aMARTINS JÚNIOR, A.
700 1 $aMACHADO, R.
700 1 $aLANDIM-ALVARENGA, F. da C.
773 $tZygote$gv. 20, p. 1-8, 2012.
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