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Registro Completo |
Biblioteca(s): |
Embrapa Acre; Embrapa Gado de Corte; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
19/12/2023 |
Data da última atualização: |
28/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SIHLER, W.; SANCHES, M. M.; FAZOLIN, M.; ESTRELA, J. L. V.; SOUZA, M. L. de. |
Afiliação: |
WILLIAM SIHLER, Cenargen; MARCIO MARTINELLO SANCHES, CNPGC; MURILO FAZOLIN, CPAF-AC; JOELMA LIMA VIDAL ESTRELA; MARLINDA LOBO DE SOUZA, Cenargen. |
Título: |
Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Technology in Agronomy, v. 4, e001, 2024. |
DOI: |
https://doi.org/10.48130/tia-0023-0019 |
Idioma: |
Inglês |
Conteúdo: |
The hornworm (Erinnyis ello) is an important pest of cassava and rubber tree with high capacity of migration and can be controlled with biological control agents such as baculoviruses. Baculoviruses are rod-shaped enveloped viruses with circular double-stranded DNA genomes infecting insects of the order Lepidoptera. Prior to large-scale use of certain baculovirus, it is essential to assess its genetic and morphological information. This work aimed to characterize the baculovirus isolate occurring in Erinnyis ello populations at small farms in Cruzeiro do Sul, Acre State, Brazil and to develop parameters for field management using this isolate. Viral particles were purified from infected larvae through ultracentrifugation in sucrose gradient. After treatment and negative staining with uranyl acetate 2%, viral particles were visualized using transmission electron microscope. The virus with ovicylindrical occlusion body presenting only one virion per envelope and size less than 0.5 µm was identified as a member of the genus Betabaculovirus. Viral DNA was extracted through phenol/chloroform cycles, digested with different restriction enzymes and separated with agarose gel electrophoresis. The restriction enzyme patterns obtained with the enzymes Bam HI and Hind III revealed seven and three DNA fragments, respectively; cleavage with Pst I, generated eight molar fragments and three submolar fragments and Eco RI digestion resulted in 21 fragments, with some submolar bands. Comparison of the DNA restriction pattern from ErelGV isolated in Acre with that described for ErelGV isolated from SC State showed high similarity. This isolate named as ErelGV-Acre presented potential for control of E. ello populations. MenosThe hornworm (Erinnyis ello) is an important pest of cassava and rubber tree with high capacity of migration and can be controlled with biological control agents such as baculoviruses. Baculoviruses are rod-shaped enveloped viruses with circular double-stranded DNA genomes infecting insects of the order Lepidoptera. Prior to large-scale use of certain baculovirus, it is essential to assess its genetic and morphological information. This work aimed to characterize the baculovirus isolate occurring in Erinnyis ello populations at small farms in Cruzeiro do Sul, Acre State, Brazil and to develop parameters for field management using this isolate. Viral particles were purified from infected larvae through ultracentrifugation in sucrose gradient. After treatment and negative staining with uranyl acetate 2%, viral particles were visualized using transmission electron microscope. The virus with ovicylindrical occlusion body presenting only one virion per envelope and size less than 0.5 µm was identified as a member of the genus Betabaculovirus. Viral DNA was extracted through phenol/chloroform cycles, digested with different restriction enzymes and separated with agarose gel electrophoresis. The restriction enzyme patterns obtained with the enzymes Bam HI and Hind III revealed seven and three DNA fragments, respectively; cleavage with Pst I, generated eight molar fragments and three submolar fragments and Eco RI digestion resulted in 21 fragments, with some submolar bands. Comparis... Mostrar Tudo |
Palavras-Chave: |
Acre; Amazonia Occidental; Amazônia Ocidental; Árbol de goma; Caterpillar; Comunidades rurales; Control biológico; Cruzeiro do Sul (AC); Cultivo a pequeña escala; Hornworm; Plagas de plantas; Rubber tree; Western Amazon; x; Yuca. |
Thesagro: |
Baculovirus; Controle Biológico; Erinnyis Ello; Hevea Brasiliensis; Lagarta; Mandioca; Manihot Esculenta; Pequeno Produtor; Praga de Planta; Propriedade Rural; Seringueira. |
Thesaurus Nal: |
Baculoviridae; Biological control; Cassava; DsDNA viruses; Insect larvae; Lepidoptera; Pest management; Plant pests; Rural communities; Small-scale farming. |
Categoria do assunto: |
-- O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1162096/1/tia-0023-0019.pdf
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Marc: |
LEADER 03389naa a2200613 a 4500 001 2162337 005 2024-02-28 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.48130/tia-0023-0019$2DOI 100 1 $aSIHLER, W. 245 $aErinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil.$h[electronic resource] 260 $c2024 520 $aThe hornworm (Erinnyis ello) is an important pest of cassava and rubber tree with high capacity of migration and can be controlled with biological control agents such as baculoviruses. Baculoviruses are rod-shaped enveloped viruses with circular double-stranded DNA genomes infecting insects of the order Lepidoptera. Prior to large-scale use of certain baculovirus, it is essential to assess its genetic and morphological information. This work aimed to characterize the baculovirus isolate occurring in Erinnyis ello populations at small farms in Cruzeiro do Sul, Acre State, Brazil and to develop parameters for field management using this isolate. Viral particles were purified from infected larvae through ultracentrifugation in sucrose gradient. After treatment and negative staining with uranyl acetate 2%, viral particles were visualized using transmission electron microscope. The virus with ovicylindrical occlusion body presenting only one virion per envelope and size less than 0.5 µm was identified as a member of the genus Betabaculovirus. Viral DNA was extracted through phenol/chloroform cycles, digested with different restriction enzymes and separated with agarose gel electrophoresis. The restriction enzyme patterns obtained with the enzymes Bam HI and Hind III revealed seven and three DNA fragments, respectively; cleavage with Pst I, generated eight molar fragments and three submolar fragments and Eco RI digestion resulted in 21 fragments, with some submolar bands. Comparison of the DNA restriction pattern from ErelGV isolated in Acre with that described for ErelGV isolated from SC State showed high similarity. This isolate named as ErelGV-Acre presented potential for control of E. ello populations. 650 $aBaculoviridae 650 $aBiological control 650 $aCassava 650 $aDsDNA viruses 650 $aInsect larvae 650 $aLepidoptera 650 $aPest management 650 $aPlant pests 650 $aRural communities 650 $aSmall-scale farming 650 $aBaculovirus 650 $aControle Biológico 650 $aErinnyis Ello 650 $aHevea Brasiliensis 650 $aLagarta 650 $aMandioca 650 $aManihot Esculenta 650 $aPequeno Produtor 650 $aPraga de Planta 650 $aPropriedade Rural 650 $aSeringueira 653 $aAcre 653 $aAmazonia Occidental 653 $aAmazônia Ocidental 653 $aÁrbol de goma 653 $aCaterpillar 653 $aComunidades rurales 653 $aControl biológico 653 $aCruzeiro do Sul (AC) 653 $aCultivo a pequeña escala 653 $aHornworm 653 $aPlagas de plantas 653 $aRubber tree 653 $aWestern Amazon 653 $ax 653 $aYuca 700 1 $aSANCHES, M. M. 700 1 $aFAZOLIN, M. 700 1 $aESTRELA, J. L. V. 700 1 $aSOUZA, M. L. de 773 $tTechnology in Agronomy$gv. 4, e001, 2024.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registros recuperados : 115 | |
21. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FERREIRA, B. C.; CASTRO, M. E. B. de; PINEDO, F. J. R.; SOUZA, M. L. de. Construction of a recombinant Anticarsia Gemmatalis MNPV with interruption of the PIF-1 gene (PER OS INFECTIVITY FACTOR). Virus Reviews & Research, São Paulo, v. 14, Supl. 1, p. 201, nov. 2009. Edição dos resumos do 20. National Meeting of Virology. Brasília, DF, nov. 2009.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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24. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CASTRO, M. E. B. de; RIBEIRO, Z. M. de A.; SIHLER, W.; SOUZA, M. L. de. Coleção de vírus de invertebrados para uso em controle biológico. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. CD-ROMTipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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34. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FERREIRA, B. C.; RIBEIRO, B. M.; CASTRO, M. E. B. de; SOUZA, M. L. de. Predição de regiões transmembranas das proteínas PIF de Anticarsia gemmatalis MNPV. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 084. p. 123Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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35. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SIHLER, W.; SOUZA, M. L. de; VALICENTE, F. H.; FALCAO, R.; SANCHES, M. M. In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines. Pesquisa Agropecuária Brasileira, Brasília, v. 53, n. 1, p. 1-9, 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Agroenergia; Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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36. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SIHLER, W.; SOUZA, M. L. de; VALICENTE, F. H.; FALCAO, R.; SANCHES, M. M. In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines. Pesquisa Agropecuária Brasileira, Brasília, v. 53, n. 1, p. 1-9, 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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37. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FERREIRA, B. C.; MELO, F. L.; RIBEIRO, B. M.; SOUZA, M. L. de. Sequenciamento do genoma de dois clones do baculovírus Anticarsia gemmatalis MNPV com variação de virulência. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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