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Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
12/02/2009 |
Data da última atualização: |
26/02/2009 |
Autoria: |
RESENDE, F. V.; SOUZA L. S. de; OLIVEIRA, P. S. R. de; GUALBERTO, R. |
Afiliação: |
Francisco Vilela Resende, Embrapa Hortaliças; Luciano Soares de Souza, Paulo Sérgio Rabello de Oliveira, Ronan Gualberto, Universidade de Marília. |
Título: |
De morta essa cobertura não tem nada. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Revista Campo & Negócios HF, Uberlândia, v. 3, n. 37 , p. 20-21, jun. 2008 |
Idioma: |
Português |
Palavras-Chave: |
Cobertura; Cultura; Morta. |
Thesagro: |
Cenoura; Daucus Carota; Germinação; Produtividade; Semente; Solo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00651naa a2200253 a 4500 001 1781089 005 2009-02-26 008 2008 bl uuuu u00u1 u #d 100 1 $aRESENDE, F. V. 245 $aDe morta essa cobertura não tem nada. 260 $c2008 650 $aCenoura 650 $aDaucus Carota 650 $aGerminação 650 $aProdutividade 650 $aSemente 650 $aSolo 653 $aCobertura 653 $aCultura 653 $aMorta 700 1 $aSOUZA L. S. de 700 1 $aOLIVEIRA, P. S. R. de 700 1 $aGUALBERTO, R. 773 $tRevista Campo & Negócios HF, Uberlândia$gv. 3, n. 37 , p. 20-21, jun. 2008
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Embrapa Hortaliças (CNPH) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
Data corrente: |
24/05/2012 |
Data da última atualização: |
22/02/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
COTTA, S. R; MOTA, F. F. da; TUPINAMBÁ, G.; ISHIDA, K.; ROZENTAL, S.; SILVA, D. O. e; SILVA, A. J. R. da; BIZZO, H. R.; ALVIANO, D. S.; ALVIANO, C. S.; SELDIN, L. |
Afiliação: |
SIMONE RAPOSO COTTA, UFRJ; FABIO FARIA DA MOTA, UFRJ; GLEISER TUPINAMBÁ, UFRJ; KELLY ISHIDA, UFRJ; SONIA ROZENTAL, UFRJ; DAVI OLIVEIRA E SILVA, UFRJ; ANTONIO JORGE RIBEIRO DA SILVA, UFRJ; HUMBERTO RIBEIRO BIZZO, CTAA; DANIELA SALES ALVIANO, UFRJ; CELUTA SALES ALVIANO, UFRJ; LUCY SELDIN, UFRJ. |
Título: |
Antimicrobial activity of Paenibacillus kribbensis POC 115 against the dermatophyte Trichophyton rubrum. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 28, n. 3, p. 953-962, 2012. |
DOI: |
10.1007/s11274-011-0893-1 |
Idioma: |
Inglês |
Conteúdo: |
In a search for an antifungal substance with activity against the dermatophyte fungus Trichophyton rubrum, strain POC 115 was chosen among different Paenibacillus strains for its phenotypic and genetic characterization and for preliminary characterization of its antimicrobial substance. Strain POC 115 was identified as belonging to Paenibacillus kribbensis. Physico-chemical characterization of the antimicrobial substance showed that it was not stable during heat and organic solvents treatments, but its activity was preserved at a wide range of pH and after treatment with pronase E, trypsin and DNase I. The crude concentrated supernatant of POC 115 culture was partially purified and the fraction presenting antimicrobial activity was further analyzed by UPLC/Mass Spectrometry. Two peaks were observed at 2.02 (mass 1,207 D) and 2.71 (mass 1,014 D) min in the mass chromatogram. The antimicrobial substance produced by POC 115 was correlated to iturin family compounds based on a set of primers designed for the amplification of PKS operon in the POC 115 genome. As happens with the mode of action of the antibiotics of the iturin group, the AMS produced by POC 115 caused the disruption of cytoplasmic membrane of T. rubrum and the subsequent withdraw of the intracellular material. This is the first report on the production of antimicrobial substances in P. kribbensis, and it may be of great relevance as an alternative or supplementary substance to antifungal drugs currently used against dermatophytes. MenosIn a search for an antifungal substance with activity against the dermatophyte fungus Trichophyton rubrum, strain POC 115 was chosen among different Paenibacillus strains for its phenotypic and genetic characterization and for preliminary characterization of its antimicrobial substance. Strain POC 115 was identified as belonging to Paenibacillus kribbensis. Physico-chemical characterization of the antimicrobial substance showed that it was not stable during heat and organic solvents treatments, but its activity was preserved at a wide range of pH and after treatment with pronase E, trypsin and DNase I. The crude concentrated supernatant of POC 115 culture was partially purified and the fraction presenting antimicrobial activity was further analyzed by UPLC/Mass Spectrometry. Two peaks were observed at 2.02 (mass 1,207 D) and 2.71 (mass 1,014 D) min in the mass chromatogram. The antimicrobial substance produced by POC 115 was correlated to iturin family compounds based on a set of primers designed for the amplification of PKS operon in the POC 115 genome. As happens with the mode of action of the antibiotics of the iturin group, the AMS produced by POC 115 caused the disruption of cytoplasmic membrane of T. rubrum and the subsequent withdraw of the intracellular material. This is the first report on the production of antimicrobial substances in P. kribbensis, and it may be of great relevance as an alternative or supplementary substance to antifungal drugs currently used again... Mostrar Tudo |
Palavras-Chave: |
Dermatófitos patogênicos; Paenibacillus kribbensis; Substância antimicrobiana. |
Thesaurus NAL: |
Trichophyton rubrum. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02433naa a2200301 a 4500 001 1925383 005 2016-02-22 008 2012 bl uuuu u00u1 u #d 024 7 $a10.1007/s11274-011-0893-1$2DOI 100 1 $aCOTTA, S. R 245 $aAntimicrobial activity of Paenibacillus kribbensis POC 115 against the dermatophyte Trichophyton rubrum. 260 $c2012 520 $aIn a search for an antifungal substance with activity against the dermatophyte fungus Trichophyton rubrum, strain POC 115 was chosen among different Paenibacillus strains for its phenotypic and genetic characterization and for preliminary characterization of its antimicrobial substance. Strain POC 115 was identified as belonging to Paenibacillus kribbensis. Physico-chemical characterization of the antimicrobial substance showed that it was not stable during heat and organic solvents treatments, but its activity was preserved at a wide range of pH and after treatment with pronase E, trypsin and DNase I. The crude concentrated supernatant of POC 115 culture was partially purified and the fraction presenting antimicrobial activity was further analyzed by UPLC/Mass Spectrometry. Two peaks were observed at 2.02 (mass 1,207 D) and 2.71 (mass 1,014 D) min in the mass chromatogram. The antimicrobial substance produced by POC 115 was correlated to iturin family compounds based on a set of primers designed for the amplification of PKS operon in the POC 115 genome. As happens with the mode of action of the antibiotics of the iturin group, the AMS produced by POC 115 caused the disruption of cytoplasmic membrane of T. rubrum and the subsequent withdraw of the intracellular material. This is the first report on the production of antimicrobial substances in P. kribbensis, and it may be of great relevance as an alternative or supplementary substance to antifungal drugs currently used against dermatophytes. 650 $aTrichophyton rubrum 653 $aDermatófitos patogênicos 653 $aPaenibacillus kribbensis 653 $aSubstância antimicrobiana 700 1 $aMOTA, F. F. da 700 1 $aTUPINAMBÁ, G. 700 1 $aISHIDA, K. 700 1 $aROZENTAL, S. 700 1 $aSILVA, D. O. e 700 1 $aSILVA, A. J. R. da 700 1 $aBIZZO, H. R. 700 1 $aALVIANO, D. S. 700 1 $aALVIANO, C. S. 700 1 $aSELDIN, L. 773 $tWorld Journal of Microbiology and Biotechnology$gv. 28, n. 3, p. 953-962, 2012.
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