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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
17/12/2018 |
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Data da última atualização: |
17/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, J. S.; CHIARI, L.; RESENDE, R. M. S.; VILELA, M. de M.; SALGADO, L. R. |
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Afiliação: |
Juliana Santos Souza, Universidade Anhanguera - Uniderp/Campus Agrárias; LUCIMARA CHIARI, CNPGC; ROSANGELA MARIA SIMEAO, CNPGC; MARIANE DE MENDONCA VILELA, CNPGC; Leonardo Rippel Salgado, CNPGC. |
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Título: |
Development, Validation and Characterization of Genic Microsatellite Markers in Urochloa Species. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
American Journal of Plant Science, v. 18, n. 3, p. 314-319, July/Sept. 2018. |
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DOI: |
DOI: 10.4236/ajps.2018.92023 |
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Idioma: |
Inglês |
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Conteúdo: |
Urochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens ?Basilisk? roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha , U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple orrespondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are the first set of expressed sequence tagged molecular markers for Urochloa species. MenosUrochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens ?Basilisk? roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha , U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple orrespondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are th... Mostrar Tudo |
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Palavras-Chave: |
Signalgrass; Simple Sequence Repeat; Transferability. |
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Thesagro: |
Brachiaria. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188572/1/8-Development-validation-and-characterization.pdf
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Marc: |
LEADER 02299naa a2200229 a 4500
001 2101685
005 2018-12-17
008 2018 bl uuuu u00u1 u #d
024 7 $aDOI: 10.4236/ajps.2018.92023$2DOI
100 1 $aSOUZA, J. S.
245 $aDevelopment, Validation and Characterization of Genic Microsatellite Markers in Urochloa Species.$h[electronic resource]
260 $c2018
520 $aUrochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens ?Basilisk? roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha , U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple orrespondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are the first set of expressed sequence tagged molecular markers for Urochloa species.
650 $aBrachiaria
653 $aSignalgrass
653 $aSimple Sequence Repeat
653 $aTransferability
700 1 $aCHIARI, L.
700 1 $aRESENDE, R. M. S.
700 1 $aVILELA, M. de M.
700 1 $aSALGADO, L. R.
773 $tAmerican Journal of Plant Science$gv. 18, n. 3, p. 314-319, July/Sept. 2018.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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| Registros recuperados : 73 | |
| 2. |  | SOUZA, J. S.; CHIARI, L.; VILELA, M. de M.; VALLE, C. B. do. Validação de marcadores microssatélites em Urochloa decumbens e avaliação da transferibilidade para outras espécies deste gênero. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 10., 2014, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2014. p. 56-57. 2 p. (Embrapa Gado de Corte. Documentos, 208). Comissão organizadora: Grácia Maria Soares Rosinha, Alexandra Rocha de Oliveir, Rodrigo Carvalho Alva.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | BONFADA, M. L.; LAMEGO, F. P.; SOUZA, J. S.; RITA, G. R.; SILVA, G. M. da. Identificação de plantas indesejadas em sementeiro de cornichão. In: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PECUÁRIA SUL, 8., 2018, Bagé. Resumos... Bagé: Embrapa Pecuária Sul, 2018. p. 27. (Embrapa Pecuária Sul. Eventos técnicos & científicos, 1). Fernando Flores Cardoso, Daniel Portella Montardo, José Carlos Ferrugem Moraes, Marcos Flávio Silva Borba, Sandro da Silva Camargo, editores técnicos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 5. | | SOUZA, J. S.; CHIARI, L.; RESENDE, R. M. S.; VILELA, M. de M.; SALGADO, L. R. Development, Validation and Characterization of Genic Microsatellite Markers in Urochloa Species. American Journal of Plant Science, v. 18, n. 3, p. 314-319, July/Sept. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 9. | | SOUZA, J. S.; LAMEGO, F. P.; BONFADA, M. L.; PEREZ, N. B. Manejo de capim-annoni com enxada química. In: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PECUÁRIA SUL, 8., 2018, Bagé. Resumos... Bagé: Embrapa Pecuária Sul, 2018. p. 29. (Embrapa Pecuária Sul. Eventos técnicos & científicos, 1). Fernando Flores Cardoso, Daniel Portella Montardo, José Carlos Ferrugem Moraes, Marcos Flávio Silva Borba, Sandro da Silva Camargo, editores técnicos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 10. | | BONFADA, M. L.; SOUZA, J. S.; RITA, G. R.; SILVA, G. M. da; LAMEGO, F. P. Manejo de Rumex crispus com enxada química. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 26.; ENCONTRO DE PÓS-GRADUAÇÃO UFPEL, 19.; SEMANA INTEGRADA DE ENSINO, PESQUISA E EXTENSÃO, 3., 2016, Pelotas. Anais... Pelotas: UFPel, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 16. | | SOUZA, J. S.; RIZZATTI, I. M.; SANTIAGO, E. R. M.; MARAGON, C. S.; SOUSA, R. de C. P. de. Avaliação da fertilidade dos solos de Várzea de Sacaí, Caracaraí, Roraima - Brasil. In: SEMANA NACIONAL DE CIÊNCIA E TECNOLOGIA NO ESTADO DE RORAIMA, SNCT-RR , 7., 2012. Boa Vista. Resumos... Boa Vista, RR: UERR, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 18. | | PEREIRA, W.; PEDULO, R. O.; PEREIRA, A.; SOUZA, J. S.; SCHULTZ, N.; URQUIAGA, S.; REIS, V. M. Extraction of N in sugarcane under increased N-fertilizer use and inoculation with fi ve diazotrophs In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16.; LATINAMERICAN WORKSHOP OF PGPR, 4.; RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Embrapa, 2018. p. 101 Session 6, Poster VI.77| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 19. | | SILVA, G. M. da; LAMEGO, F. P.; TRENTIN, G.; RITA, G. R.; SOUZA, J. S.; BONFADA, M. L. Corte pré-florescimento na produção de sementes de trevo branco (Trifolium repens L.). Informativo Abrates, v. 27, n. 2, p. 300, ago. 2017. Edição dos anais do 20º Congresso Brasileiro de Sementes, Foz do Iguaçu.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| Registros recuperados : 73 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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