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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Trigo. |
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Data corrente: |
06/05/1997 |
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Data da última atualização: |
25/11/2019 |
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Autoria: |
SOUZA, I. R. P. de. |
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Afiliação: |
EMBRAPA/CNPMS. |
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Título: |
Gibberellic acid and dwarfism effects on peroxidase activity and secretion of anionic isoenzymes into the cell wall of expanding maize (Zea mays L.) leaf blades. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Logan: Utah State University, 1997. |
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Páginas: |
89p. |
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Idioma: |
Inglês |
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Notas: |
Doctor Sc. Thesis. |
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Conteúdo: |
Many studies have related changes in plant growth with peroxidase activity, but few of these have correlated changes in growth with changes in a specific isoenzyme, although many isoenzymes have been shown to be organ-specific with developmental expression. We used the developmental gradient presented by the maize (Zea mays L.) leaf blade to study the spatial distribution of growth and peroxidase activity. All experimentation used the second expanding true leaf blade in which the ligule ahd not been displaced from the leaf base by growth of the leaf sheath. Quantitative and qualitative analyses of total soluble (soluble plus ionically bound to the cell wall) and apoplastic peroxidase activity were conducted along the leaf blade of the B73 maize inbred line. For apoplastic fluid extraction, a vacuum-infiltration procedure was used followed by low-speed centrifugation. No cytoplasmic contamination occurred because no glucuose-6-phosphate dehydrogenase activity could be detected in apoplastic extracts. Total soluble peroxidase activity presented was high near the leaf base in the region of cell division, and relatively low through the rest of the elongation zone. In contrast, a transient peak in apoplastic peroxidase activity occurred in the middle of the elongation zone, ans poceded a arpid decrease in cell elongation rate. Zymograms of total and apoplastic extraction showed that two anionic peroxidase isoenzymes, pI 5.6 and 5.7 began to increase in activity where epidermal cell elongation stopped. (...) MenosMany studies have related changes in plant growth with peroxidase activity, but few of these have correlated changes in growth with changes in a specific isoenzyme, although many isoenzymes have been shown to be organ-specific with developmental expression. We used the developmental gradient presented by the maize (Zea mays L.) leaf blade to study the spatial distribution of growth and peroxidase activity. All experimentation used the second expanding true leaf blade in which the ligule ahd not been displaced from the leaf base by growth of the leaf sheath. Quantitative and qualitative analyses of total soluble (soluble plus ionically bound to the cell wall) and apoplastic peroxidase activity were conducted along the leaf blade of the B73 maize inbred line. For apoplastic fluid extraction, a vacuum-infiltration procedure was used followed by low-speed centrifugation. No cytoplasmic contamination occurred because no glucuose-6-phosphate dehydrogenase activity could be detected in apoplastic extracts. Total soluble peroxidase activity presented was high near the leaf base in the region of cell division, and relatively low through the rest of the elongation zone. In contrast, a transient peak in apoplastic peroxidase activity occurred in the middle of the elongation zone, ans poceded a arpid decrease in cell elongation rate. Zymograms of total and apoplastic extraction showed that two anionic peroxidase isoenzymes, pI 5.6 and 5.7 began to increase in activity where epidermal ce... Mostrar Tudo |
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Palavras-Chave: |
Aluminium; Growth; Leaf; Maize; Tolerance; Tolerancia. |
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Thesagro: |
Ácido Giberélico; Alumínio; Biologia Molecular; Crescimento; Eletroforese; Fisiologia; Folha; Isoenzima; Milho; Regulador de Crescimento; Zea Mays. |
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Thesaurus Nal: |
molecular biology; plant physiology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02514nam a2200361 a 4500
001 1474415
005 2019-11-25
008 1997 bl uuuu m 00u1 u #d
100 1 $aSOUZA, I. R. P. de
245 $aGibberellic acid and dwarfism effects on peroxidase activity and secretion of anionic isoenzymes into the cell wall of expanding maize (Zea mays L.) leaf blades.
260 $aLogan: Utah State University$c1997
300 $a89p.
500 $aDoctor Sc. Thesis.
520 $aMany studies have related changes in plant growth with peroxidase activity, but few of these have correlated changes in growth with changes in a specific isoenzyme, although many isoenzymes have been shown to be organ-specific with developmental expression. We used the developmental gradient presented by the maize (Zea mays L.) leaf blade to study the spatial distribution of growth and peroxidase activity. All experimentation used the second expanding true leaf blade in which the ligule ahd not been displaced from the leaf base by growth of the leaf sheath. Quantitative and qualitative analyses of total soluble (soluble plus ionically bound to the cell wall) and apoplastic peroxidase activity were conducted along the leaf blade of the B73 maize inbred line. For apoplastic fluid extraction, a vacuum-infiltration procedure was used followed by low-speed centrifugation. No cytoplasmic contamination occurred because no glucuose-6-phosphate dehydrogenase activity could be detected in apoplastic extracts. Total soluble peroxidase activity presented was high near the leaf base in the region of cell division, and relatively low through the rest of the elongation zone. In contrast, a transient peak in apoplastic peroxidase activity occurred in the middle of the elongation zone, ans poceded a arpid decrease in cell elongation rate. Zymograms of total and apoplastic extraction showed that two anionic peroxidase isoenzymes, pI 5.6 and 5.7 began to increase in activity where epidermal cell elongation stopped. (...)
650 $amolecular biology
650 $aplant physiology
650 $aÁcido Giberélico
650 $aAlumínio
650 $aBiologia Molecular
650 $aCrescimento
650 $aEletroforese
650 $aFisiologia
650 $aFolha
650 $aIsoenzima
650 $aMilho
650 $aRegulador de Crescimento
650 $aZea Mays
653 $aAluminium
653 $aGrowth
653 $aLeaf
653 $aMaize
653 $aTolerance
653 $aTolerancia
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Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
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| Registros recuperados : 515 | |
| 22. |  | MARUCCI, R. C.; MENDES, S. M.; SOUZA, I. L. Maize. In: SOUZA, B.; VÁZQUEZ, L. L.; MARUCCI, R. C. (Ed.). Natural enemies of insect pests in neotropical agroecosystems: biological control and functional biodiversity. Switzerland: Springer Nature, 2019. p. 329-339.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 32. | | SOUZA, I. N. G.; LAMEIRA, O. A.; SILVA, E. R. M.; COSTA, K. J. A. Avaliação fenológica da quina (Quassia Amara L.) simaroubaceae. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 21., 2017, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 34. | | COSTA, K. J. A.; LAMEIRA, O. A.; SOUZA, I. N. G.; MAVÃO, L. dos S. Aspectos fenológicos do açoita-cavalo - Luehea speciosa Willd. (Malvaceae). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 21., 2017, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 35. | | SILVA, E. R. M.; LAMEIRA, O. A.; SOUZA, I. N. G.; CAMPELO, M. F. Aspectos fenológicos de Alternanthera dentata (Moench) Stuchlik. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 21., 2017, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 36. | | MAGALHAES, P. C.; OLIVEIRA, E. de; GOMIDE, R. L.; VASCONCELOS, C. A.; SOUZA, I. R. P. Aspectos fisiológicos de plantas de milho infectadas por molicutes sob diferentes níveis de água no solo. Revista Brasileira de Fisiologia Vegetal, Lavras, v. 13, n. 3, p. 293-301, 2001. VASCONCELLOS, C.A.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 38. | | PORTAL, R. K. V. P.; LAMEIRA, O. A.; SOUZA, I. N. G.; COSTA, A. da S. Aspecto fenológico da espécie Phyla betulifolia (Kunth) Greene. (Verbenaceae). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 21., 2017, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 515 | |
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