Data/hora: 04/06/2024 06:32:47

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 18/07/2005
Data da última atualização: | 18/07/2005
Autoria: | DE SOUZA, F. A.; DECLERCK, S.; SMIT, E.; KOWALCHUK, G. A.
Título: | Morphological, ontogenetic and molecular characterization of Scutellospora reticulata (Glomeromycota).
Ano de publicação: | 2005
Fonte/Imprenta: | Mycological Research, New York, v. 109, n. 6, p. 697-706, jun. 2005.
Idioma: | Inglês
Conteúdo: | The arbuscular mycorrhizal (AM) fungus Scutellospora reticulata (CNPAB11) was characterized using morphological, ontogenetic and molecular aproaches. Spore ontogenesis was using Ri T-DNA transformed carrots and obeservations were compared with those published for eight other, pot-cultured, Scutellospora species. The sporogenesis of S. reticulata exhibited an unreoprted parttern of outherspore wall differentiation. In addition, Denaturing Gradient Gel Electrophoresis (DGGE),targeting the V9 region of the SSU nrDNA, was used to differentiate S. reticulata from 16 other Scutellospora species and species and results were confirmed by sequencing analysis. Phylogenetic analyses, using nearly full length SSU nrDNA sequences, grouped S reticulata in a cluster together with S cerradensis and S. heterogama, species that share similar spore wall organization and posses ornamented external walls. PCR-DGGE and sequence analysis revealed intragenomic SSU nrDNA oplymorphism in four out of six Scutellospora species, and demostrated that SSU nrDNA intragenomic polymorphism could de used as a marker to differentiate several closely related Scutellospora species.
Thesagro: | Micologia; Micorriza Vesicular Arbuscular.
Thesaurus Nal: | mycology; vesicular arbuscular mycorrhizae.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (32657-1) - UPC - SP - -- -  -  - 0022861 - DIS