Data/hora: 10/06/2024 14:17:51

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 01/03/2004
Data da última atualização: | 14/05/2004
Autoria: | SOUZA, F. A. de; DECLERCK, S.
Título: | Mycelium development and architecture, and spore production of Scutellospora reticulata in monoxenic culture with Ri T-DNA transformed carrot roots.
Ano de publicação: | 2003
Fonte/Imprenta: | Mycologia, Bronx, v. 95, n. 6, p. 1004-1012, 2003.
Idioma: | Inglês
Conteúdo: | Mycelium development and architecture and spore production were studied in Scutellospora reticulata from single-spore isolates grown with Ri T- DNA transformed carrot root-organ culture in monoxenic system. Culture establishment, anastomosis occurrence and auxiliary cell development also were examined. Seventy percent of the pregerminated disinfected spores colonized the transformed carrot roots. After 8 mo, the average spore production was 56 (24-130) per 30 cm3 of medium. Of the spores produced, 75% germinated and produced new generations in monoxenic culture. The mycelium net- work was formed by thick light-brown hyphae, which exhibit two major architecture patterns related to either root colonization or resource exploitation, and lower-order hyphae, bearing auxiliary cells, branched absorbing structures (BAS) , hyphal swellings (HS) and forming anastomoses. BAS were formed abundantly in extramatrical mycelium and frequently had HS resembling vesicles, a feature not previously re- ported in the Gigasporaceae, to the best of our knowledge. Few anastomosis were observed within the mycelium and most often corresponded to a healing mechanism that form hypha bridges to reconnect broken hyphae or overcoming obstructed areas within a hypha. Numerous auxiliary cells were produced during culture development and their role was inferred.
Palavras-Chave: | Root; Scutellospora reticulata; Vesicular arbuscular mycorrhizal.
Thesagro: | Cenoura; Micorriza Vesicular Arbuscular; Raiz.
Thesaurus Nal: | carrots; roots; vesicular arbuscular mycorrhizae.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)

Exemplares:

CNPAB - (29290-1) - UPC - SP - -- -  -  - 22703 - DIS
CNPAB - (29869-1) - UMT - SP - -- -  -  - 22705 - DIS