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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
10/07/2018 |
Data da última atualização: |
18/09/2018 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
SOUZA, F. V. D.; SOUZA, E. H. de; SILVA, R. L. da. |
Afiliação: |
FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; EVERTON HILO DE SOUZA; RONILZE LEITE DA SILVA. |
Título: |
Cryopreservation of pollen grains of pineapple and other bromeliads. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: LOYOLA-VARGAS, V.r M; Ochoa-Alejo, N. (ed.). Plant Cell Culture Protocols, Methods in Molecular Biology. New York, NY: Humana Press, 2018. |
Páginas: |
279-288 |
Descrição Física: |
v.1815. Cap. 19. |
DOI: |
0.1007/978-1-4939-8594-4_19 |
Idioma: |
Inglês |
Conteúdo: |
Cryopreservation of pollen grains is an efficient technique to overcome asynchronous flowering and to support actions for genetic improvement and conservation of important alleles. It can be used both by germplasm curators and plant breeders. In the case of Bromeliaceae, a family with wide diversity but also high vulnerability, the form of conservation can be crucial to prevent the increasing problem of genetic erosion. This chapter describes a method of cryopreservation of pollen grains of different Bromeliaceae species, including pineapple, after dehydration with silica and subsequent immersion in liquid nitrogen. The efficiency of this protocol has been demonstrated by the high pollen viability percentage and produc-tion of seeds after in vivo pollination with cryopreserved grains. The protocol can be used for cryopreserv-ing pollen of many species of bromeliads and is easy to perform. |
Thesagro: |
Bromeliaceae; Conservação; Criopreservação; Melhoramento Vegetal. |
Thesaurus Nal: |
Breeding and Genetic Improvement; Conservation plants; Cryopreservation. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01763naa a2200253 a 4500 001 2093161 005 2018-09-18 008 2018 bl uuuu u00u1 u #d 024 7 $a0.1007/978-1-4939-8594-4_19$2DOI 100 1 $aSOUZA, F. V. D. 245 $aCryopreservation of pollen grains of pineapple and other bromeliads.$h[electronic resource] 260 $c2018 300 $a279-288$cv.1815. Cap. 19. 520 $aCryopreservation of pollen grains is an efficient technique to overcome asynchronous flowering and to support actions for genetic improvement and conservation of important alleles. It can be used both by germplasm curators and plant breeders. In the case of Bromeliaceae, a family with wide diversity but also high vulnerability, the form of conservation can be crucial to prevent the increasing problem of genetic erosion. This chapter describes a method of cryopreservation of pollen grains of different Bromeliaceae species, including pineapple, after dehydration with silica and subsequent immersion in liquid nitrogen. The efficiency of this protocol has been demonstrated by the high pollen viability percentage and produc-tion of seeds after in vivo pollination with cryopreserved grains. The protocol can be used for cryopreserv-ing pollen of many species of bromeliads and is easy to perform. 650 $aBreeding and Genetic Improvement 650 $aConservation plants 650 $aCryopreservation 650 $aBromeliaceae 650 $aConservação 650 $aCriopreservação 650 $aMelhoramento Vegetal 700 1 $aSOUZA, E. H. de 700 1 $aSILVA, R. L. da 773 $tIn: LOYOLA-VARGAS, V.r M; Ochoa-Alejo, N. (ed.). Plant Cell Culture Protocols, Methods in Molecular Biology. New York, NY: Humana Press, 2018.
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16. | | SILVA, M. de J. da; SOUZA, E. H. de; SOUZA, F. V. D. Produção de mudas de abacaxi ornamental para validação de cultivo comercial. In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 17.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 4.; 2009, Aracaju. Ciência, inovação e sustentabilidade: [anais...]. Aracaju: Embrapa Tabuleiros Costeiros; Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM. (Embrapa Tabuleiros Costeiros. Documentos, 150). Organizadores: Ana da Silva Lédo; Fernanda Vidigal D. Souza; Vivian Loges; Everton Hilo de Souza; Ana Cecília R. de Castro.Tipo: Resumo em Anais de Congresso |
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